Comparison of biological characteristics between menstrual blood mesenchymal stem cells and human umbilical cord mesenchymal stem cells

doi:10.3969/j.issn.1007-3205.2023.12.002

Abstract

Abstract: Objective To compare the biological characteristics of menstrual blood mesenchymal stem cells (MenSCs) with human umbilical cord mesenchymal stem cells (hUCMSCs).
Methods Mesenchymal stem cells (MSCs) were isolated from menstrual blood and umbilical cord. The surface markers and cell proliferation of two kinds of stem cells were compared by flow cytometry, immunocytochemistry and CCK8 assay. Experimental groups were divided into control group, MenSCs-CM group, and hUCMSCs-CM group. The effects of two kinds of stem cells on the proliferation, migration and angiogenesis on human umbilical vein endothelial cells (HUVECs) were detected by CCK8 assay, scratch assay and angiogenesis assay.
Results Both kinds of stem cells adhered to the wall and were fusiform. There was high expression of CD90, CD44, CD73, CD105, low expression of CD14, CD45, CD34, and no expression of HLA-DR. Both MenSCs and hUCMSCs expressed SOX2 and VEGFA, but MenSCs had highly expressed OCT4. The results showed that proliferation, migration and angiogenesis of HUVECs in both the MenSCs-CM group and hUCMSCs-CM group were higher than those in the control group (P＜0.05). The MenSCs-CM group was superior to the hUCMSCs-CM group with respect to proliferation and angiogenesis of HUVECs (P＜0.05).
Conclusion Both kinds of cells have the properties of mesenchymal stem cells, but MenSCs also have the properties of embryonic stem cells. Both MenSCs and hUCMSCs can promote the proliferation, migration and angiogenesis of HUVECs, but the effect of MenSCs is better than hUCMSCs.

Key words: mesenchymal stem cells, endothelial cells, proliferation, angiogenesis

SUN Cong, XUE Guo-yu, ZHOU Gui-yuan, LI Jing-ze, CHEN Wei, SHAO Su-xia. Comparison of biological characteristics between menstrual blood mesenchymal stem cells and human umbilical cord mesenchymal stem cells [J]. Journal of Hebei Medical University, 2023, 44(12): 1370-1378.

[1]	FAN Long-kun, LIU Chun-hui, ZHAO Yu-jia, MA Chao. Study on the mechanism of BMSC-exos inhibiting hypertrophic scar through TGF-β1/smad2/3 signal pathway [J]. Journal of Hebei Medical University, 2023, 44(8): 876-882,899.
[2]	FAN Yu-tong, BAI Zhao-rong, ZHANG Yao-guo, LI Peng-fei, GAO Jun-ling, ZHOU Hong-xia. Effect of adipose derived mesenchymal stem cells on autophagy in rats with intracerebral hemorrhage and its mechanism of action [J]. Journal of Hebei Medical University, 2023, 44(5): 502-511.
[3]	WANG Xu, XU Jing, HUA Fang, WANG Yu-jing, DU Zhi-xiang. lncRNA SNHG12 promotes mirgation, invasion and suppresses apoptosis of cervical cancer SiHa cells [J]. Journal of Hebei Medical University, 2023, 44(5): 547-552.
[4]	ZHANG Jia-hui, MA Hong-yun, QIN Juan. The effect of silencing miR-26b viaPI3K/Akt pathway on proliferation and apoptosis of placental trophoblastic cells in patients with pregnancy-induced hypertension [J]. Journal of Hebei Medical University, 2021, 42(9): 1063-1067.
[5]	ZHANG Ning, QIN Xi-jing, ZHU Yu-lin, LI Pei, HUANG Xiang-hua. Effect of CM-Dil labeling on bone marrow stromal stem cells and its tracking in rat model of vaginal reconstruction [J]. Journal of Hebei Medical University, 2021, 42(8): 872-875,封三.
[6]	LI Gang, XIAO Han, TANG Jun-jian, WANG Feng, YOU Qing-jun. Experimental study on isolation and culture of canine vascular endothelial cells by collagenase digestion scrape and repeat digestion techniques [J]. Journal of Hebei Medical University, 2021, 42(1): 7-11.
[7]	CHI Wei-wei1, NIU Ying-hao1, DONG Li-ping1, ZHANG Ji-hua2, WU Guang-li1, SONG Dong-mei1*. Changes of endothelial progenitor cells in peripheral blood of patients with obstructive sleep apnea hypopnea syndrome [J]. Journal of Hebei Medical University, 2020, 41(9): 1025-1028.
[8]	ZHAO Juan, LI Long*. Down regulation of MCT1 expression inhibits proliferation and promotes apoptosis of Hela cells#br# [J]. Journal of Hebei Medical University, 2020, 41(5): 570-573,578.
[9]	XU Yan-nan, MENG Li, ZHU Yan, YAN Jing, WANG Yan-ling, ZHOU Chen-ming. Effect of dihydroartemisinin on proliferation and apoptosis of human gastric cancer cell line BGC-823 and its mechanisms [J]. Journal of Hebei Medical University, 2020, 41(11): 1245-1250.
[10]	PANG Ya-xiang1, HAN Hua2, YIN Yue1, MA Guo-hua3, NIU Hong-lin4*. Overexpression of miR-140-5p promotes apoptosis and inhibits proliferation of prostate cancer cells#br# [J]. Journal of Hebei Medical University, 2020, 41(1): 7-11.
[11]	ZUO Hongling1, YAN Xiaonan2, LIU Subin3, LI Xiuwen1, WANG Huilan1, XU Chunlin1, DU Hui1*. The effects of 17βestradiol on proliferation of ovarian clear cell carcinoma ES2 cell line and the possible mechanism [J]. Journal of Hebei Medical University, 2019, 40(9): 1029-1033.
[12]	ZHANG Yanan1， CHEN Wei1， ZHANG Jinping1， QI Lili2， ZHANG Lei1， ZHAO Chunfang1*. Study on differentiation from bone marrow mesenchymal stem cells overexpressing GATA4 into cardiomyocytes [J]. Journal of Hebei Medical University, 2019, 40(8): 877-881,901.
[13]	NIE Hongfeng1， ZHANG Ping2， HE Xuexia1， CAO Qing1， HOU Yanqing1， MENG Lei1. miR19a3p regulates the proliferation of gastric cancer cells via targeting PTEN#br# [J]. Journal of Hebei Medical University, 2019, 40(5): 538-542,546.
[14]	LIU Sha, WU Ming, DI Kun, JIA Jing, WU Qiaona, LIU Zengjuan*. Human umbilical cord mesenchymal stem cells conditioned medium protected against amyloid β induced apoptosis in cultured cortical neuron of rat partly through inhibiting mitochondrial apoptotic pathway [J]. Journal of Hebei Medical University, 2019, 40(4): 390-395,410.
[15]	ZHANG Yanan1， XIE Yanyan2， YIN Mingjie2， ZHANG Xiaoyi2， CHEN Wei1， ZHANG Lei1*. GATA4 gene promote 5azacytidine induced differentiation of bone marrow mesenchymal stem cells into cardiomyocytes#br# [J]. Journal of Hebei Medical University, 2019, 40(4): 385-389.

Comparison of biological characteristics between menstrual blood mesenchymal stem cells and human umbilical cord mesenchymal stem cells

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments