Journal of Hebei Medical University ›› 2022, Vol. 43 ›› Issue (2): 130-134.doi: 10.3969/j.issn.1007-3205.2022.02.002

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Screening of differential metabolites in plasma of mice due to lethal acute poisoning with chlorpromazine hydrochloride

  

  1. 1.Grade 2017, College of Forensic Medicine, Hebei Medical University, Hebei Province, Shijiazhuang 
    050017, China; 2.College of Forensic Medicine, Hebei Medical University, Hebei Key Laboratory of 
    Forensic Medicine, Collaborative Innovation Center of Forensic Medical Molecular Identification, 
    Hebei Province, Shijiazhuang 050017, China; 3.School of Chemical and Pharmaceutical 
    Engineering, Hebei University of Science and Technology, Shijiazhuang 050018, China
  • Online:2022-02-25 Published:2022-03-03

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Abstract: Objective To analyze and screen out differential metabolites related to lethal acute poisoning with chlorpromazine hydrochloride by non-targeted metabolomics. 
Methods Twenty-four ICR mice were randomly selected and divided into saline control group(n=12) and chlorpromazine hydrochloride test group(n=12). Plasma samples of the two groups were detected by ultra-high performance liquid chromatography-high resolution mass spectrometry(UPLC-HRMS). The differential metabolites related to lethal acute poisoning with chlorpromazine hydrochloride between two groups were screened by orthogonal partial least squares-discriminant analysis(OPLS-DA), t-test and fold change statistical methods and compared with the database, thereby identifying statistically significant differential metabolites. 
Results The results of OPLS-DA analysis of plasma samples showed significant differences between the two groups. A total of 29 differential metabolites containing 11 categories such as fatty acyl groups and carboxylic acids and their derivatives were identified. 
Conclusion The metabolomics technology based on UPLC-HRMS can screen differential metabolites related to lethal acute poisoning with chlorpromazine hydrochloride, which may provide a reference for future forensic determination of the cause of death from chlorpromazine hydrochloride poisoning.


Key words: chlorpromazine, poisoning, metabolomics