Journal of Hebei Medical University ›› 2022, Vol. 43 ›› Issue (2): 135-139,192.doi: 10.3969/j.issn.1007-3205.2022.02.003

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The effect of macrophages depletion by clodronate liposome on homing of hematopoietic stem/progenitor cells

  

  1. 1.Core Research Laboratory, the Second Affiliated Hospital of Xi′an Jiaotong University, Shaanxi 
    Province, Xi′an 710004, China; 2.Department of Rheumatism and Immunology, the Second Affiliated 
    Hospital of Xi′an Jiaotong University, Shaanxi Province, Xi′an 710004, China; 3. Tianjin Medical 
    University Cancer Institute and Hospital, National Clinical Research Center for Cancer, 
    Tianjin 300060, China
  • Online:2022-02-25 Published:2022-03-03

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Abstract: Objective To explore the effect of macrophage depletion on the homing process of hematopoietic stem/progenitor cells(HSPC). 
Methods Mouse HSPCs were obtained by immunomagnetic bead sorting, and labeled with carboxyfluorescein diacetate succinimide ester(CSFE). A mouse model of macrophage depletion was established by clodronate liposomes(Clod), and the efficiency of HPSC homing was detected by flow cytometry.The mRNA and protein expression levels of CXCL12, SCF and vascular adhesion molecule 1(VCAM1) molecules in bone marrow niche after macrophage depletion were detected by real-time RT polymerase chain reaction(qRT-PCR) and enzyme linked immuno sorbent assay(ELISA) assay. 
Results The number of CD11b+F4/80+ macrophages in the bone marrow of mice was significantly decreased at the 24 and 48 h after the injection of Clod(P＜0.05). After transplantation of mouse HSPC, the homing efficiency of HSPC in the mice in the Clod group was significantly reduced, as compared with the control group(P＜0.05). In the meantime, the mRNA and protein expressions of CXCL12 and SCF in the bone marrow niche of the mice in the Clod group were significantly lower than those in the control group(P＜0.05), while there was no significant change in VCAM1 expression between Clod and control group. 
Conclusion Macrophage depletion can reduce the homing efficiency of HSPC, which may be related to the reduction of CXCL12 and SCF levels in the bone marrow niche after macrophage depletion.


Key words: myeloid progenitor cells； macrophage, clodronate liposomes