Journal of Hebei Medical University ›› 2022, Vol. 43 ›› Issue (6): 627-631.doi: 10.3969/j.issn.1007-3205.2022.06.002

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Effects of down-regulation of miR-206 on fracture healing of rat rib fracture model via PI3K/Akt signaling pathway

  

  1. 1.Department of Anesthesiology, the Second Hospital of Zhangjiakou, Hebei Province, 
    Zhangjiakou 075000, China； 2.Department of Orthopedics, the Second Hospital of 
    Zhangjiakou， Hebei Province, Zhangjiakou 075000, China
  • Online:2022-06-25 Published:2022-06-28

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Abstract: Objective To observe the effects of miR-206 and PI3K/Akt in rib fracture healing in rats. 
Methods Thirty-two SPF SD rats were used for construction of rib fracture model, and then randomly divided into miR-206 group(n=16) and control group(n=16). Eight rats were sacrificed in each group at 14 d and 28 d. Micro-CT was performed to observe fracture healing. The expression levels of PI3K and Akt were detected by qPCR, and the protein levels of PI3K and Akt were detected by Western blot. 
Results The rats were basically in good condition after the operation, and all the rats were successfully modeled. The micro-CT three-dimensional reconstruction analysis showed that the total callus volume, bone volume, and bone volume/ tissue volume(BV/TV) of rats in each group gradually increased over time. At 14 d and 28 d, there were significant differences in callus formation-related indexes between the miR-206 group and the control group(P＜0.05). The results of qPCR and Western blot showed that miR-206 inhibitor injection could significantly increase the expression and protein levels of PI3K and Akt at 14 d and 28 d, and the differences were statistically significant(P＜0.05). 
Conclusion  Down-regulation of miR-206 can activate the PI3K/Akt pathway to promote the growth and ossification of the callus at the rib fracture site, thereby promoting fracture healing.


Key words: rib fractures, miR-206, PI3K, Akt