Loading...

Table of Content

    25 June 2022, Volume 43 Issue 6
    Effects of down-regulation of miR-206 on fracture healing of rat rib fracture model via PI3K/Akt signaling pathway
    WANG Qiang, GAO Shu-jun, ZHAO Peng-fei, LIU Yan
    2022, 43(6):  627-631.  doi:10.3969/j.issn.1007-3205.2022.06.002
    Asbtract ( 317 )   PDF (529KB) ( 96 )  
    Related Articles | Metrics
    Objective To observe the effects of miR-206 and PI3K/Akt in rib fracture healing in rats. 
    Methods Thirty-two SPF SD rats were used for construction of rib fracture model, and then randomly divided into miR-206 group(n=16) and control group(n=16). Eight rats were sacrificed in each group at 14 d and 28 d. Micro-CT was performed to observe fracture healing. The expression levels of PI3K and Akt were detected by qPCR, and the protein levels of PI3K and Akt were detected by Western blot. 
    Results The rats were basically in good condition after the operation, and all the rats were successfully modeled. The micro-CT three-dimensional reconstruction analysis showed that the total callus volume, bone volume, and bone volume/ tissue volume(BV/TV) of rats in each group gradually increased over time. At 14 d and 28 d, there were significant differences in callus formation-related indexes between the miR-206 group and the control group(P<0.05). The results of qPCR and Western blot showed that miR-206 inhibitor injection could significantly increase the expression and protein levels of PI3K and Akt at 14 d and 28 d, and the differences were statistically significant(P<0.05). 
    Conclusion  Down-regulation of miR-206 can activate the PI3K/Akt pathway to promote the growth and ossification of the callus at the rib fracture site, thereby promoting fracture healing.

    Effect of medical education platform on neurological function and quality of life of patients with ischemic stroke
    WANG Juan, ZHANG Hai-jiao, GAO Yuan-yuan, TANG Li-mei, TIAN Hui-jie, TIAN Su-zhai
    2022, 43(6):  632-636,661.  doi:10.3969/j.issn.1007-3205.2022.06.003
    Asbtract ( 267 )   PDF (620KB) ( 57 )  
    Related Articles | Metrics
    Objective To explore the effect of medical education platform based on WeChat on neurological function and quality of life of patients with ischemic stroke. 
    Methods Using convenient sampling method, 430 discharged patients with ischemic stroke in Department of Neurology were selected and numbered by envelope method. The odd number was the control group and the even number was the observation group, with 215 patients in each group. The control group was given conventional health education, while the observation group was given medical education platform based on WeChat. The improved Rankin scale(mRS) and stroke-specific quality of life scale were used to compare the improvement of quality of life, neurological function and limb function of patients before discharge, at 3 months and 6 months after discharge in the two groups. 
    Results At 3 months and 6 months after intervention, the mRS scores of patients in both groups were lower than those before intervention, and the differences of interaction between groups, time points, and time points between groups were statistically significant(P<0.05). At 3 months and 6 month safter intervention, the total scores of all areas of quality of life in the two groups were increased compared with those before intervention, and the total scores of quality of life were increased; there were statistical differences in interaction between groups, time points, and time points between groups(P<0.05). 
    Conclusion The application of medical education platform based on WeChat in the transitional care of patients with ischemic stroke is helpful to reduce their mRS scores, improve the prognosis of limb function and neurological function, and improve the quality of life of patients. 

    Impact of cardiac valve calcification on mortality in patients undergoing maintenance hemodialysis
    GUAN Ren-ping, YIN Li-yong, LIU Wei-ping, LI Tao, GUO Yong-li, ZHANG Yan-fen
    2022, 43(6):  637-640,675.  doi:10.3969/j.issn.1007-3205.2022.06.004
    Asbtract ( 384 )   PDF (620KB) ( 80 )  
    Related Articles | Metrics
    Objective To analyze the impact of clinical condition, examination and laboratory results,especially cardiac valve calcification, on mortality of patients undergoing maintenance hemodialysis in our center in the past three years. 
    Methods A total of 443 patients undergoing hemodialysis in our hospital were analyzed. The first recorded data and clinical findings of dead and alive patients were compared. 
    Results Among all patients, 298 patients survived and 145 died. Dead patients had an older mean age, 60% of dead patients had coronary heart disease, and 53.8% had cardiac valve calcification. The duration of hemodialysis was shorter in dead patients. Alkaline phosphatase,albumin,urea clearance index(Kt/V)were lower, while C-reactive protein,full-length parathyroid hormone and the potassium level before hemodialysis were higher in dead patients.Logistic regression analysis showed that age, coronary artery disease(CAD)and multiple cardiac valve calcification(≥2) were independent risk factors for mortality. Potassium level before hemodialysis, full-length parathyroid hormone and higher Kt/V were conductive to survival. 
    Conclusion Age, CAD and multiple cardiac valve calcification are risk factors for mortality of patients undergoing maintenance hemodialysis.

    Level and correlation of CysC, IL-2 and C3 in patients with chronic glomerular nephropathy
    YAO Peng-yun, GONG Ya-ping, GAO Jia-qi
    2022, 43(6):  641-645,650.  doi:10.3969/j.issn.1007-3205.2022.06.005
    Asbtract ( 406 )   PDF (634KB) ( 107 )  
    Related Articles | Metrics
    Objective To analyze the level and correlation of Cystatin C(CysC), interleukin-2(IL-2) and C3 in patients with chronic glomerular nephropathy and to provide reference for the clinical diagnosis of this disease. 
    Methods A total of 138 patients with chronic glomerular nephropathy were selected as the research group, and 80 healthy physical examinees in our hospital as the control group. Serum CysC, IL-2 and C3 levels were detected, and their expression differences in the two groups and in patients with different severities of disease were analyzed. The correlation among the three was analyzed. 
    Results Compared with the control group, CysC levels were higher, while IL-2 and C3 levels were lower in the research group(P<0.05). The ROC curve showed that combined detection of CysC, IL-2 and C3 had a higher diagnostic value for chronic glomerular nephropathy than CysC, IL-2 and C3 alone(P<0.05). Univariate analysis showed that the incidence of chronic glomerular nephropathy was not related to the patient's gender, age, serum uric acid, and comorbidities, with no significant difference(P>0.05), but was related to smoking history, drinking history, obesity, CysC, IL-2 and C3 levels(P<0.05). Correlation analysis showed that CysC was positively correlated with renal function damage(eGFR)(r=0.404, P<0.001); IL-2 was negatively correlated with eGFR(r=-0.375, P<0.001); C3 was negatively correlated with eGFR(r=-0.504, P<0.001). Chronic glomerular nephropathy served as dependent variables, and significant variables of univariate analysis as independent variables were incorporated into the biclassification multi factor Logistic regression model, which showed that CysC, IL-2, and C3 were influencing factors of the occurrence of chronic glomerular nephropathy(P<0.05). CysC was negatively correlated with IL-2(r=-0.250, P=0.003); CysC was negatively correlated with C3(r=-0.373, P=0.001); IL-2 was positively correlated with C3(r=0.232, P=0.006). 
    Conclusion Serum CysC levels are increased, while IL-2 and C3 levels are decreased in patients with chronic glomerular nephropathy, which is related to eGFR of the patients. The disease can be diagnosed and evaluated early based on their level.

    The effect of miR-188-5p on the improvement of insulin-resistant Hep G2 cells
    XU Jin-e, ZHANG Zheng
    2022, 43(6):  646-650.  doi:10.3969/j.issn.1007-3205.2022.06.006
    Asbtract ( 411 )   PDF (830KB) ( 69 )  
    Related Articles | Metrics
    Objective To investigate the effect of miR-188-5p on insulin resistance of Hep G2 cells. 
    Methods Hep G2 cells were induced and treated with high concentration of glucose(30 mmol/L) for 24 h, to establish insulin resistance model. The experiment was divided into 6 groups: control group, model group, miR-188-5p mimic group, mimic-NC group, miR-188-5p inhibitor group, and inhibitor-NC group. The expression of miR-188-5p in Hep G2 cells was detected by qPCR, and the proliferation rate of Hep G2 cells was measured by MTT assay. The contents of glucose and glycogen in Hep G2 cells were detected by biochemical experiments, and the expression of glucose transporter type 4(GLUT4) was observed by immunofluorescence staining. 
    Results Compared with the control group, miR-188-5p expression, cell proliferation rate, glucose uptake and glycogen content in the model group were significantly reduced(P<0.05), and GLUT4 protein expression was reduced. Compared with the model group, the expression of miR-188-5p, cell proliferation rate, glucose uptake and glycogen content of miR-188-5p mimic group were significantly increased(P<0.05), and the expression of GLUT4 protein was also increased, as opposed to miR-188-5p inhibitor group. 
    Conclusion The expression of miR-188-5p is decreased in insulin-resistant Hep G2 cells. Up-regulation of miR-188-5p expression can promote cell proliferation and alleviate glucose metabolism disorders.

    The expression of STC1, TWEAK, and miR-183 in COPD patients and their correlation
    JIANG Li-ping, LI Wen-zhen, WEI Jiang-mei
    2022, 43(6):  651-656,670.  doi:10.3969/j.issn.1007-3205.2022.06.007
    Asbtract ( 377 )   PDF (588KB) ( 104 )  
    Related Articles | Metrics
    Objective To investigate the expression of stanniocalcin 1(STC1), tumor necrosis factor-like weak inducer of apoptosis(TWEAK), and microRNA(miRNA)-183 in patients with chronic obstructive pulmonary disease(COPD) and their correlation. 
    Methods In total, 114 COPD patients were selected as the COPD group, and divided into severe(n=23), moderate(n=48) and mild(n=43) groups according to the degree of airflow restriction, and divided into death(n=22) and survival(n=92) groups according to the prognosis. Another 63 healthy people undergoing physical examination during the same period were selected as the control group.The lung function [forced expiratory volume in one second(FEV1), FEV1/forced vital capacity(FVC)]of the subjects was examined, and the levels of serum STC1, TWEAK, miR-183, malondialdehyde(MDA), superoxide dismutase(SOD), interleukin(IL) 6 and IL-8 were determined. Pearson correlation analysis was used to analyze the correlation between serum STC1, TWEAK, miR-183 levels and lung function and serum MDA, SOD, IL-6, and IL-8 levels in COPD group. The ROC curve was used to analyze the predictive value of serum STC1, TWEAK, and miR-183 levels in the poor prognosis of COPD patients. 
    Results Compared with the control group, FEV1, FEV1/FVC and serum STC1, SOD levels in the COPD group were significantly reduced, while the levels of TWEAK, miR-183, MDA, IL-6, and IL-8 were significantly increased(P<0.05).Serum STC1 and SOD levels in the mild, moderate and severe groups decreased sequentially, while the levels of TWEAK, miR-183, MDA, IL-6, and IL-8 increased sequentially(P<0.05). Serum STC1 levels in the COPD group were positively correlated with FEV1, FEV1/FVC, and SOD, and negatively correlated with MDA, IL-6, and IL-8(P<0.05). TWEAK and miR-183 levels were negatively correlated with FEV1, FEV1/FVC, and SOD, and positively correlated with MDA, IL-6, and IL-8(P<0.05). The area under ROC curve (AUC) of STC1+TWEAK+miR-183(AUC=0.919, 95%CI: 0.853-0.862) in predicting poor prognosis of COPD patients was significantly greater than that of STC1(AUC=0.796, 95%CI: 0.710-0.866), TWEAK(AUC=0.788, 95%CI: 0.702-0.859), and miR-183(AUC=0.798, 95%CI: 0.713-0.868) alone(P<0.05).
    Conclusion The serum STC1 level of patients with COPD decreases, and the levels of TWEAK and miR-183 increase. It may be involved in the progression of COPD through oxidative stress and inflammation, which can be used as predictors of poor prognosis for patients.

    Evaluation of the effect of enhanced recovery after surgery concept on prevention of complications after total hip arthroplasty
    LI Shu-qiang, DING Kai, LI Sai, WANG Ya-dong, CHEN Wei, LI Chun-liu
    2022, 43(6):  657-661.  doi:10.3969/j.issn.1007-3205.2022.06.008
    Asbtract ( 456 )   PDF (402KB) ( 185 )  
    Related Articles | Metrics
    Objective To study the effect of enhanced recovery after surgery(ERAS) method on the prevention of complication saftertotal hip arthroplasty(THA). 
    Methods Patients who underwent THA from the Third Hosipital of Hebei Medical Univerisity were selected as research subjects. According to the principle of random control, the patients were divided into research group and control group. The research group was given ERAS nursing program, while the control group was given conventional nursing. The visual analogue scale(VAS) pain scores, postoperative complications(deep venous thrombosis, urinary tract infection, falling pneumonia, pressure sores, constipation) and Harris hip function scorewere recorded. The length of hospitalstay, average hospitalization expenses and patient satisfaction were compared. 
    Results VAS scores of the two groups decreased over time, and the difference of interaction between groups, time points and time points between groups were statistically significant(P<0.05). The incidence of complications in the research group was lower than that in the control group, while Harris score was higher than that in the control group, with significant difference(P<0.05). The length of hospital stay and hospitalization expenses in the research group were less than those in the control group, while the patient satisfaction was higher than that in the control group, suggesting significant difference(P<0.05). 
    Conclusion ERAS can reduce the incidence of postoperative complications, promote recovery of patients after THA, and improve the satisfaction of patients and their families. 


    Analysis of influencing factors of postoperative pregnancy outcome in patients with moderate to severe ovarian endometriosis treated with ultrasound-guided puncture combined with leuprolide
    LIU Xiang-ju, HAN Jie, GOU Ming-yue, ZHAO Yan-hong, YANG Lu
    2022, 43(6):  662-665,710.  doi:10.3969/j.issn.1007-3205.2022.06.009
    Asbtract ( 378 )   PDF (413KB) ( 162 )  
    Related Articles | Metrics
    Objective To study the influencing factors of postoperative pregnancy outcome in patients with moderate to severe ovarian endometriosis treated by ultrasound-guided puncture combined with leuprolide. 
    Methods In total, 120 patients with severe ovarian endometriosis admitted to our hospital were enrolled in this study. All patients underwent ultrasound-guided puncture, and 62 patients in the leuprorelin group were supplemented with leuprorelin after operation. The patients were followed up and their pregnancy rate was recorded. The age, duration of infertility, diameter of cyst, single/multiple cyst, endometriosisfertility index(EFI) and preoperative CA125, serum anti-Müllerian hormone(AMH) level, bilateral/unilateral ovarian involvement of patients with successful pregnancy and pregnancy failure were recorded. 
    Results At the end of follow-up, all patients were visited, and the follow-up rate was 100%. The pregnancy rate of leuprorelin group was higher than that of non-leuprorelin group(P<0.05). The recurrence rate of leuprorelin group was lower than that of non-leuprorelin group(P<0.05). Binary Logistic regression analysis showed that the use of GnRHa after operation, the level of preoperative AMH, bilateral ovarian involvement and the presence of multiple cysts were the independent influencing factors of pregnancy success(P<0.05). 
    Conclusion Ultrasound-guided puncture combined with leuprorelin is effective in the treatment of moderate to severe ovarian endometriosis. The use of GnRHa, preoperative AMH level, bilateral ovarian involvement, and the presence of multiple cysts are the influential factors that affect the success of pregnancy after surgery. 

    Study on the relationship between serum 25-hydroxyvitamin D levels and food allergy in infants
    ZHAO Hui, REN Yan-yan, ZHANG Hui-feng
    2022, 43(6):  666-670.  doi:10.3969/j.issn.1007-3205.2022.06.010
    Asbtract ( 537 )   PDF (384KB) ( 116 )  
    Related Articles | Metrics
    Objective To investigate the relationship between serum 25-hydroxyvitamin D levels and food allergy in infants, and to analyze the risk factors for food allergy. 
    Methods A total of 40 infants aged ≤3 years with a diagnosis of food allergy were enrolled as the research group, while 40 age-matched healthy children were selected as the control group. Serum 25-hydroxyvitamin D levels were compared between two groups, and the potential risk factors for food allergy were analyzed. 
    Results There was no significant difference in the level of serum 25-hydroxyvitamin D between two groups(P>0.05). The proportion of children with vitamin D deficiency and insufficiency was higher in the food allergy group(P<0.05). There was no significant difference in serum 25-hydroxyvitamin D level between children with IgE-mediated food allergy and those with non-IgE-mediated food allergy(P>0.05). Univariate Logistic regression analysis showed that family history of allergic diseases, eczema history and low vitamin D level might be the risk factors for food allergy(P<0.05). Multivariate Logistic regression analysis showed that eczema history was an independent risk factor for food allergy(P<0.05). 
    Conclusion Serum vitamin D deficiency and insufficiency is more common in children with food allergy, but there is no significant difference in serum 25-hydroxyvitamin D levels between children with food allergy and healthy children. Infants with a history of eczema are at higher risk of developing food allergy. 

    Logistic regression analysis of influencing factors and prevention and treatment strategies for PICC-related thrombosis during induction chemotherapy in children with acute leukemia
    HE Cai-hong, LIU Ai-ming
    2022, 43(6):  671-675.  doi:10.3969/j.issn.1007-3205.2022.06.011
    Asbtract ( 458 )   PDF (407KB) ( 181 )  
    Related Articles | Metrics
    Objective To analyze the influencing factors of peripherally inserted central catheter (PICC)-related thrombosis during induction chemotherapy in children with acute leukemia by Logistic regression analysis. 
    Methods A total of 184 children with acute leukemia were retrospective selected. According to the presence or absence of thrombosis at the catheter site during induction chemotherapy period(at 15 d after catheter placement), they were divided into thrombus group(n=38) and the non-thrombosis group(n=146). The clinical data of the two groups were collected, and univariate and multivariate Logistic regression analyses were used to analyze the influencing factors of PICC-related thrombosis during induction chemotherapy in children with acute leukemia. The receiver operating characteristic(ROC) curve was used to evaluate the predictive value of multivariate Logistic regression model in PICC-related thrombosis in children with acute leukemia during induction chemotherapy. 
    Results There was no significant difference between two groups in age, sex, disease type, disease risk, vein catheterization, as well as white blood cell(WBC), platelet(PLT), D-dimer(D-D), prothrombin time(PT), and activated partial thromboplastin time(APTT) at the time of catheterization, and WBC, PLT, PT and APTT at 15 d after catheterization(P>0.05). There were significant differences in catheter placement, catheter-related infection, use of hemostatic drugs and D-D level at 15 d after catheterization between two groups(P<0.05). Multivariate Logistic regression analysis showed that right-sided catheterization, catheter-related infection, use of hemostatic drugs, and high D-D level at 15 d after catheterization were independent risk factors for PICC-related thrombosis in children with acute leukemia during induction chemotherapy(P<0.05). ROC curve analysis showed that the area under the ROC curve(AUC) of multivariate Logistic regression model in predicting PICC-related thrombosis in children with acute leukemia during induction chemotherapy was 0.917, 95%CI was 0.866 to 0.954, sensitivity was 84.21%, and specificity was 84.44%. 
    Conclusion There are a number of independent risk factors for PICC-related thrombosis during induction chemotherapy in children with acute leukemia. Multivariate Logistic regression model has a reliable predictive value, which is conductive to reduction of the risk of PICC-related thrombosis.

    Risk factors of secondary CNSI in pediatric intensive care unit in department of neurosurgery
    ZHANG Zheng-xia, ZHANG Zheng-hong, LUO Ping, Long Dan, Xin Yan-mei
    2022, 43(6):  676-681.  doi:10.3969/j.issn.1007-3205.2022.06.012
    Asbtract ( 403 )   PDF (433KB) ( 99 )  
    Related Articles | Metrics
    Objective To analyze the situation of secondary central nervous system infection(CNSI) in Pediatric Intensive Care Unit(PICU) in Department of Neurosurgery, and to study the risk factors for secondary infection.
    Methods The clinical data of 825 children in PICU were analyzed. The children were divided into CNSI group and non-CNSI group according to their CNSI. Univariate analysis was performed for the differences in indicators between two groups, and multivariate Logistic analysis was performed for the risk factors of secondary CNSI in children in PICU in Department of Neurosurgery. 
    Results The incidence of CNSI in children in PICU in Department of Neurosurgery was 3.03%. There were significant differences in age, concomitant diabetes mellitus(DM), Glasgow Coma Scale(GCS) score, leucopenia, length of hospital stay between the CNSI group and the non-CNSI group(P<0.05). There were statistically significant differences in the duration of operation, subtentorial craniotomy, typeⅠ surgical incision between the CNSI group and the non-CNSI group(P<0.05). There were significant differences in invasive operation, indwelling site and indwelling time between the CNSI group and the non-CNSI group(P<0.05). Significant differences were found in systemic antibiotics and staphylococcus aureus infection between the CNSI group and the non-CNSI group(P<0.05).The independent risk factors for CNSI in patients in PICU included age<8 years, concomitant DM, GCS score<8 points, leukopenia, length of hospital stay≥10 d, or duration ofoperation≥4 h, subtentorial craniotomy, invasive operation, indwelling drainage tube outside the ventricle,drainage tube indwelling time≥7 d, systemic application of antibiotics, or staphylococcus aureus infection. 
    Conclusion There are various risk factors for CNSI in children in PICU in Department of Neurosurgery. Therefore, optimized surgical plan, personalized treatment, reduced duration of operation and length of hospital stay, standardized invasive operation,regular infection prevention of indwelling tube, and rational use of antibiotics can effectively prevent the occurrence of CNSI.

    Changes in OCTA before and after intravitreal injection of anti-VEGF drugs in eyes with wet age-related macular degeneration and its correlation with visual acuity
    XU Yu-neng, SHEN Yi-fei, RONG Fei
    2022, 43(6):  682-687.  doi:10.3969/j.issn.1007-3205.2022.06.013
    Asbtract ( 347 )   PDF (2730KB) ( 81 )  
    Related Articles | Metrics
    Objective To explore the changes in optical coherence tomography angiography(OCTA) of eyes with wet age-related macular degeneration(wAMD) before and after intravitreal injection of anti-vascular endothelial growth factor(VEGF) drugs and its correlation with visual acuity. 
    Methods The data of 72 wAMD patients(72 eyes) who received intravitreal injection of anti-VEGF drugs were retrospectively analyzed, and the changes of cross sectional area(CSA), cross flow area(CFA), and central macular thickness(CMT) before and after treatment were compared. The standard logarithmic visual acuity chart was used to check the patient's best corrected visual acuity(BCVA) before and after treatment, which was converted into LogMAR visual acuity chart values. Pearson correlation analysis was used to analyze the relationship between difference of CSA, CFA, CMT and difference of BCVA(LogMAR) before and after treatment with anti-VEGF drugs in wAMD eyes, and the therapeutic effect of patients was calculated. Logistic regression analysis was used to analyze the factors influencing the ineffectiveness of anti-VEGF drug treatment in wAMD eyes. 
    Results CSA, CFA and CMT after anti-VEGF drugs in wAMD eyes were lower than those before treatment(P<0.05). BCVA(LogMAR) after anti-VEGF drug treatment in wAMD eyes was lower than that before treatment(P<0.05). According to Pearson correlation analysis, difference of CSA, CFA, CMT before and after anti-VEGF drug treatment in wAMD eyes were positively correlated with difference of BCVA(LogMAR)(P<0.05). Logistic multivariate regression analysis showed that preoperative intraocular pressure, pretreatment CSA, CFA, and CMT were related factors that affected the ineffectiveness of anti-VEGF drug treatment in wAMD eyes(P<0.05). 
    Conclusion Difference of CSA, CFA, CMT before and after treatment with anti-VEGF drugs in wAMD eyes are positively correlated with difference of BCVA(LogMAR) before and after treatment, and CSA, CFA, and CMT are all related factors that affect the therapeutic effect of anti-VEGF drugs in wAMD eyes.
    Therapeutic effect of low-temperature plasma adenoidectomy combined with bilateral tonsil regimen in the treatment of pediatric snoring and its influencing factors
    LI Wen, HAO Zhong-ping, WANG Cheng, XIE Wei-pin, TIAN Chun-hui, GU Huai-yu
    2022, 43(6):  688-692,715.  doi:10.3969/j.issn.1007-3205.2022.06.014
    Asbtract ( 420 )   PDF (445KB) ( 123 )  
    Related Articles | Metrics
    Objective To explore the effect of low-temperature plasma adenoidectomy combined with bilateral tonsil regimen in the treatment of pediatric snoring and its influencing factors. 
    Methods In total, 110 children with snoring were selected as the research subjects. According to the random number table method, they were divided into the control group and the observation group, with 55 cases in each group. The cure rate and total effective rate were observed, and the immunoglobulin A(IgA), immunoglobulin G(IgG), and immunoglobulin M(IgM) were compared before surgery, and at 1 and 6 months after surgery. The apnea hypopnea index(AHI), minimum blood oxygen saturation[LSa(O2)] and pediatric sleep questionnaire(PSQ) scores before and at 7 d after surgery were assessed. 
    Results The total effective rate and cure rate of the observation group were higher than those of the control group(P<0.05). The duration of operation, intraoperative blood loss, duration of postoperative pain and symptom relief time in the observation group were shorter or less than those in the control group(P<0.05). The levels of IgA, IgG, and IgM in both groups increased with time, which were higher in the observation group than in the control group at 1 month and 6 months after surgery(P<0.05). There were statistically significant differences in the interaction between groups, time points and time points between groups with respect to IgA, IgG and IgM(P<0.05). Simple effect LSD-t pairwise comparison showed that there was no significant difference in IgA, IgG, IgM, AHI, LSa(O2) and PSQ scores between two groups before surgery(P>0.05); at 1 month and 6 months after surgery, IgA, IgG, and IgM in the observation group were higher than those in the control group(P<0.05). At 7 d after surgery, the AHI of the observation group was lower than that of the control group, while the LSa(O2) and PSQ scores were higher than those of the control group(P<0.05). There was no difference in gender, age, course of disease, weight, preoperative IgA, preoperative IgG, preoperative IgM, preoperative AHI, preoperative LSa(O2), and preoperative PSQ scores between the cured/markedly effective group and the effective/ineffective group(P>0.05), while there was significant significance in comparison of surgical methods(P<0.05). Logistic regression analysis showed that adenoidectomy with the power cutting system combined with conventional bilateral tonsillectomy was an independent risk factor affecting the therapeutic effect of pediatric snoring(P<0.05). 
    Conclusion Low-temperature plasma adenoidectomy combined with bilateral tonsillectomy is effective in the treatment of pediatric snoring. It not only has a little impact on the immune function, but also improves the ventilation function of the patient.

    Effect of trazodone hydrochloride combined with BiPAP ventilation on serum PPAR-γ, YKL-40 and vWF levels in patients with obstructive sleep apnea hypopnea syndrome
    GUO Li-na, ZHU Xi-min, GU Shu-he
    2022, 43(6):  693-697.  doi:10.3969/j.issn.1007-3205.2022.06.015
    Asbtract ( 426 )   PDF (415KB) ( 89 )  
    Related Articles | Metrics
    Objective To observe the effect of trazodone hydrochloride combined with noninvasive bi-level positive airway pressure(BiPAP) ventilation on serum peroxisome proliferator activated receptor-γ(PPAR-γ), chitinase-like protein(YKL-40) and von Willebrand factor(vWF) levels in patients with obstructive sleep apnea hypopnea syndrome(OSAHS). 
    Methods In total, 162 patients with OSAHS were selected and randomly divided into the control group and the research group, with 81 cases in each group. The control group was given BiPAP ventilation treatment, and the research group was given trazodone hydrochloride combined with BiPAP ventilation treatment. Both groups were treated for 40 d. The efficacy, Pittsburgh sleep quality scale(PSQI) score, Calgary quality of life index(SAQLI) for OSAHS patients, apnea hypopnea index(AHI), lowest oxygen saturation(LSaO2), mean oxygen saturation(MSaO2) and serum PPAR-γ, YKL-40, vWF and the incidence of adverse reactions were compared between two groups. 
    Results After treatment, the total effective rate of the research group was significantly better than that of the control group(P<0.05). After treatment, the PSQI score of the research group was lower than that of the control group, while the SAQLI score was higher than that of the control group(P<0.05). After treatment, AHI in the research group was lower than that in the control group, while LSaO2 and MSaO2 in the research group were higher than those in the control group(P<0.05). After treatment, the serum PPAR-γ, YKL-40 and vWF in the research group were lower than those in the control group(P<0.05). There was no difference in the total incidence of adverse reactions between two groups(P>0.05). 
    Conclusion Trazodone hydrochloride combined with BiPAP ventilation in the treatment of OSAHS patients can help ameliorate the symptoms, enhance the quality of life by improving the sleep quality of patients, and reduce the serum PPAR-γ, YKL-40and vWF levels.

    Effect of tooth-whitening products on hardness of demineralized and stained teeth after orthodontic treatment
    LU Lin, GUO Zhi-juan, QIE Hui, LI Chen-xi, ZHANG Fan, SHAN Li-huan
    2022, 43(6):  698-701.  doi:10.3969/j.issn.1007-3205.2022.06.016
    Asbtract ( 463 )   PDF (356KB) ( 69 )  
    Related Articles | Metrics
    Objective To observe the effect of tooth-whitening products on the hardness of demineralized and stained teeth after orthodontic treatment. 
    Methods In total,120 premolars extracted due to orthodontic treatment were used to establish early demineralization models. They were randomly divided into four groups(n=30): Opalescence tooth-whitening products group(group A), beyond tooth-whitening products group(group B), duraphat group(group C), and blank control group(group D). The changes in color scale and enamel microhardness were estimated. 
    Results ①Colorimetric palette: The color scale values of each group gradually decreased and then increased with time, and the difference in color scale values in time points between groups was statistically significant(P<0.05). ②Surface microhardness value(SMV): The change of SMV value of group A showed a trend of initial increasing and then decreasing with time, and SMV values in the other three groups increased initially with time and then tended to be stable. Comparison between groups showed that except the absence of statistical differences between group A and group C, the difference between the remaining groups was statistically significant(P<0.05). 
    Conclusion Opalescence Boost teeth-whitening products and beyond teeth-whitening products can remineralize the demineralized teeth.Opalescence Boost teeth-whitening products have a slightly higher degree of enamel mineralization than Beyond teeth-whitening products.

    CBCT study on the improvement of upper airway in adolescents with skeletal class Ⅱ mandibular retrusion treated with modified muscle activator
    FENG Yan, ZHANG Liang, LIU Hao, TANG Na
    2022, 43(6):  702-705,726.  doi:10.3969/j.issn.1007-3205.2022.06.017
    Asbtract ( 519 )   PDF (772KB) ( 53 )  
    Related Articles | Metrics
    Objective To analyze the clinical effect of modified muscle activator in improving up per airway in adolescents with skeletal Class II mandibular retrusion. 
    Methods A total of 60 adolescents with skeletal class Ⅱ mandibular retrusion admitted to our hospital were selected as the research subjects, and randomly divided into research group(n=30) and control group(n=30) by random number table method. The control group was treated with TWIN-BLOCK(TB), and the research group was treated with modified muscle activator. The changes in upper airway in both groups were compared. 
    Results After treatment, SHP, STE and SMIN in the research group were greater than those before treatment, while ST and SMIN in the control group were greater than those before treatment(P<0.05). LHP, LSP, LTE, APHP, APSP and APTE in the research group were greater than those before treatment(P<0.05), and LSP in the research group was greater than that in the control group(P<0.05). AHI in the research group was lower than that before treatment, while lowest oxygen saturation(LSaO2) was higher than that before treatment; AHI was lower than that in the control group, while LSaO2 was higher than that in the control group(P<0.05). 
    Conclusion The volume of upper airway in adolescents with skeletal Class Ⅱ mandibular retrusion treated with modified muscle activator is significantly increased, the inner diameter and cross-sectional area are enlarged, and the ventilation condition of upper airway as well as indicatorsof OSAS is improved. CBCT was used for clinical analysis, so as to provide data support for making the optimal surgical plan, ameliorating the problems of patients′ upper airway, evaluating the morphology and mechanism of upper airway, and making personalized treatment plan.

    Predictive value of carotid plaque vulnerability assessed by contrast-enhanced ultrasound combined with three-dimensional ultrasound in acute ischemic stroke
    ZHANG Li-wei, CHEN Xue, WANG Yi-cheng, GAO Xin-chun
    2022, 43(6):  706-710.  doi:10.3969/j.issn.1007-3205.2022.06.018
    Asbtract ( 398 )   PDF (537KB) ( 57 )  
    Related Articles | Metrics
    Objective To investigate the predictive value of carotid plaque vulnerability assessed by contrast-enhanced ultrasound combined with three-dimensional ultrasound in acute ischemic stroke(AIS). 
    Methods A total of 261 outpatients or inpatients with carotid plaque thickness ≥2.0 mm detected after routine ultrasound examinations in our hospital were selected. According to the clinical diagnosis of AIS, all patients were divided into AIS group(n=126) and non-AIS group(n=135). Preferred carotid plaques were examined by contrast-enhanced ultrasound and three-dimensional ultrasound. The contrast-enhanced ultrasound quantitative parameter enhanced intensity(EI) and three-dimensional ultrasound quantitative parameters of carotid plaque thickness(PT), plaque volume(PV), percent area stenosis(PAS) and gray-scale median(GSM) were obtained. The above parameters were compared between two groups. The receiver operating characteristic(ROC) curve was used to analyze the predictive value of the parameters alone or in combination with statistical significance in AIS between two groups, and the cut-off value was determined. 
    Results The EI value of carotid plaque in AIS group was higher than that in non-AIS group, and the GSM value was lower than that in non-AIS group(all P<0.05); There was no significant difference in PT, PV and PAS of carotid plaque between two groups(all P>0.05). Taking EI of 11.6 as cut-off value for predicting AIS, the area under curve was 0.730, and the sensitivity and specificity were 76.19% and 62.22%, respectively; Taking GSM of 33.5 as cut-off value for predicting AIS, the area under curve was 0.752, and the sensitivity and specificity were 78.57% and 62.22%, respectively; The area under curve of combined detection in predicting AIS was 0.840, and the sensitivity and specificity were 85.71% and 71.11%, respectively. 
    Conclusion The combined detection of EI and GSM can accurately evaluate the vulnerability of carotid plaque and has a good predictive value for AIS.
    The effect of ultrasound-guided laser and radiofrequency ablation on serum tumor markers in the treatment of small liver cancer and the evaluation by contrast-enhanced ultrasound
    WANG Hai-xia, LIU Jing-ping, XU Juan
    2022, 43(6):  711-715.  doi:10.3969/j.issn.1007-3205.2022.06.019
    Asbtract ( 451 )   PDF (644KB) ( 41 )  
    Related Articles | Metrics
    Objective To explore the effect of ultrasound-guided laser and radiofrequency ablation on serum tumor markers in the treatment of small liver cancer and the evaluation by contrast-enhanced ultrasound. 
    Methods Seventy-six patients with small liver cancer were selected as the research subjects, and divided into the observation group(ultrasound-guided laser ablation treatment, n=38) and the control group(ultrasound-guided radiofrequency ablation therapy, n=38). Complete ablation rate(CAR)at 1 month after treatment was compared, and alpha-fetoprotein(AFP), abnormal prothrombinⅡ(PIVKA-Ⅱ), and glypican 3(CPC3)before treatment and at 4 weeks after treatment were evaluated.The complications(local pain, intraabdominal bleeding, biliary bleeding, bile leakage, abdominal infection), and 2-year survival rate, 2-year progression-free survival(PFS) rate and survival time were observed. 
    Results The 1-month CAR of the observation group was higher than that of the control group(P<0.05). Before treatment, there was no statistically significant difference between two groups with respect to AFP, PIVKA-Ⅱ, and CPC3(P>0.05); After treatment, the AFP, PIVKA-Ⅱ, and CPC3 of the observation group were lower than those of the control group(P<0.05). The incidence of local pain, intraabdominal bleeding, and biliary bleeding in the observation group was lower than that in the control group(P<0.05). The incidence of bile leakage and abdominal infection in the observation group was lower than that in the control group, but the difference was not statistically significant(P>0.05).The 2-year survival rate, 2-year PFS rate and survival time of the observation group were higher than those of the control group(P<0.05). 
    Conclusion Ultrasound-guided laser ablation and radiofrequency ablation are both effective methods for the treatment of small liver cancer. However, ultrasound-guided laser ablation is more effective, which can reduce the expression of serum tumor markers and increase CAR.

    Application value of combined detection of BRD4, SSBP1 and PCDH17 in the diagnosis of early gastric cancer
    YIN Xiao-xia, LI Yong, BAI Rui, LIU Bo, SUN Gai-xia
    2022, 43(6):  716-720.  doi:10.3969/j.issn.1007-3205.2022.06.020
    Asbtract ( 420 )   PDF (431KB) ( 130 )  
    Related Articles | Metrics
    Objective To explore the combined detection of bromodomain protein 4(BRD4), mitochondrial single-stranded DNA binding protein(SSBP1) and proto-cadherin 17(PCDH17) in the diagnosis of early gastric cancer. 
    Methods We collected 50 cases of gastric cancer tissues, 50 cases of precancerous tissues, and 50 cases of normal gastric mucosa tissues. The expression of BRD4, SSBP1 and PCDH17 proteins in tissue specimens was determined by immunohistochemical staining, followed by analysis in different tissue samples. The three groups of blood samples were collected to detect related serum indicators in the three groups. Receiver operating characteristic(ROC) curve was used to analyze the value of BRD4, SSBP1 and PCDH17 in the diagnosis of gastric cancer. 
    Results BRD4 and SSBP1 were highly expressed in gastric cancer tissues, while PCDH17 was poorly expressed. Comparison of the positive rates of BRD4, SSBP1 and PCDH17 in the three groups of tissue samples was statistically significant(P<0.05). The serum carbohydrate antigen(CA199), pepsinogen(PG)Ⅰ, PGⅡ, gastrin-17(G-17) levels and positive rate of helicobacter pylori(HP) in the three groups were significantly different(P<0.05). The serum CA199, PGⅠ, G-17 levels and HP positive rate of patients in gastric cancer tissue group were significantly higher than those in precancerous tissue group and normal gastric mucosal tissue group, while PGⅡ level was lower than that that in precancerous tissue group and normal gastric mucosal tissue group(P<0.05). The positive rates of BRD4, SSBP1 and PCDH17 in gastric cancer tissues of patients with clinical stage Ⅲ to Ⅳ and lymph node metastasis were significantly different(P<0.05). The sensitivity and specificity of combined detection of BRD4+SSBP1+PCDH17 were higher than an indicator alone. 
    Conclusion Combined detection of BRD4, SSBP1 and PCDH17 is conductive to the differential diagnosis of gastric cancer.

    Effect of chlorogenic acid, c-di-GMP and human bactericidal peptide LL-37 on drug resistance of Pseudomonas aeruginosa biofilm
    ZHANG Yan, ZHAO Shu-zhen, HE Jing, ZHANG Ting
    2022, 43(6):  721-726.  doi:10.3969/j.issn.1007-3205.2022.06.021
    Asbtract ( 440 )   PDF (1207KB) ( 147 )  
    Related Articles | Metrics
    Objective To investigate the role of chlorogenic acid, c-di-GMP and human bactericidal peptide LL-37 in biofilm formation(BF) of Pseudomonas aeruginosa(PA) and drug resistance. 
    Methods Wild-type pseudomonas aeruginosa strain(PAO1) was selected to carry out the study, and the minimum inhibitory concentration(MIC) of chlorogenic acid, c-di-GMP and human germicide LL-37 on PA was determined by broth dilution method. Crystal violet quantitative method was used to detect the scavenging effects of chlorogenic acid, c-di-GMP and human germicidal peptide LL-37 in PA-BF formation, and PA motion test was used to detect the effects of chlorogenic acid, c-di-GMP and human germicidal peptide LL-37 on PA motion ability. The effects of chlorogenic acid, c-di-GMP and human bactericidal peptide LL-37 on the structure and survival of PA-BF were observed under fluorescence microscope. 
    Results The value of MIC of human germicidal peptide LL-37, chlorogenic acid and c-di-GMP was 64 mg/L, 256 mg/L, and 32 mg/L, respectively. Compared with the c-divGMP group, the value of OD590nm human germicidal peptide LL-37 group, chlorogenic acid group and blank group significantly decreased, which was significantly lower in human bactericidal peptide LL-37 group and chlorogenic acid group(P<0.05). Compared with the negative control group, the PA diameter in exercise testing of the human germicidal peptide LL-37 group, the chlorogenic acid group and the c-di-GMP group significantly decreased, which was significantly lower in human germicidal peptide LL-37 group and the chlorogenic acid group, as compared with the c-di-GMP group(P<0.05).The survival of PA in blank group and c-divGMP group was better than that in human germicidal peptide LL-37 group and chlorogenic acid group. The fluorescence in blank group and c-di-GMP group was mainly green, while the fluorescence in human germicidal peptide LLv37 group and chlorogenic acid group was mainly red and orange.Compared with c-di-GMP group, the drug resistance rate of human germicidal peptide LL-37 group, negative control group and chlorogenic acid group significantly decreased, and compared with the negative control group, the drug resistance rate of human germicidal peptide LL-37 group and chlorogenic acid group significantly decreased(P<0.05). 
    Conclusion Both chlorogenic acid and human bactericidal peptide LL-37 can clear PA-BF to a certain extent, and c-di-GMP can regulate the BF formation of PA by inhibiting cell motor ability. Chlorogenic acid, human bactericidal peptide LL-37 and c-di-GMP can be used as a new direction for subsequent prevention and control of PA-BF.