Journal of Hebei Medical University ›› 2022, Vol. 43 ›› Issue (11): 1261-1266,1276.doi: 10.3969/j.issn.1007-3205.2022.11.005

Previous Articles     Next Articles

Expression and correlation of miR-21, Caveolin-1 and Gal-9 in patients with viral meningitis

  

  1. 1.The Second Department of Neurology, the First Hospital of Handan City, Hebei Province, Handan 
    056002, China; 2.The Third Department of Neurology, the Central Hospital of Handan City, 
    Hebei Province, Handan 056001, China; 3.Department of Medical Administration, 
    the First Hospital of Handan City, Hebei Province, Handan 056002, China
  • Online:2022-11-25 Published:2022-12-28

Abstract: Objective To investigate the characteristics of the expression level of micro ribonucleic acid-21(miR-21), Caveolin-1 and galectin-9(Gal-9) in cerebrospinal fluid of patients with viral encephalitis(VM), and to analyze the relationship between miR-21, Caveolin-1, Gal-9 and VM recurrence. 
Methods A total of 132 patients with VM(VM group) and 136 patients without neurological injury confirmed by examination(control group) were selected. VM patients were divided into severe group(n=58) and mild group(n=74) according to their condition. All patients were followed up for 3 months after discharge and divided into recurrence group(n=26) and non-recurrence group(n=106) according to recurrence of VM. The levels of serum miR-21, Caveolin-1, Gal-9 and inflammatory factors were detected before and after treatment, and the relationship between miR-21, Caveolin-1, Gal-9 and VM severity, inflammatory factors and recurrence was analyzed. Receiver operating characteristic(ROC) curve was used to analyze the value of miR-21, Caveolin-1 and Gal-9 in predicting VM recurrence. 
Results The expression of miR-21 and the levels of Caveolin-1, Gal-9, tumor necrosis factor-α(TNF-α) and interleukin-6(IL-6) in VM group were higher than those in control group(P<0.05). The expression of miR-21 and the levels of Caveolin-1, Gal-9, TNF-α and IL-6 in cerebrospinal fluid of VM patients in severe group were higher than those in mild group(P<0.05).The levels of miR-21, Caveolin-1 and Gal-9 were positively correlated with TNF-α and IL-6(P<0.05). Before and after treatment, the expression of miR-21 and the levels of Caveolin-1, Gsl-9, TNF-α and IL-6 in cerebrospinal fluid of the recurrence group were higher than those of the non-recurrence group. After treatment, the expression of miR-21 and the levels of Caveolin-1, Gal-9, TNF-α and IL-6 in cerebrospinal fluid of patients in recurrence group and non-recurrence group were lower than those before treatment(P<0.05). After treatment, high levels of TNF-α, miR-21, Caveolin-1 and Gal-9 were risk factors for VM recurrence(P<0.05). The area under the ROC curve(AUC) of combined detection of miR-21, Caveolin-1 and Gal-9 in predicting VM recurrence was 0.858, which was higher than that of single index(P<0.05). 
Conclusion The expression levels of miR-21, Caveolin-1 and Gal-9 in the cerebrospinal fluid of VM patients are significantly increased, and the high expression of miR-21, Caveolin-1 and Gal-9 is closely related to the severity and recurrence of VM after treatment.


Key words: encephalitis, viral, micro ribonucleic acid-21, caveolin-1, galectin-9