Journal of Hebei Medical University ›› 2022, Vol. 43 ›› Issue (11): 1255-1260.doi: 10.3969/j.issn.1007-3205.2022.11.004

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The effect of SPHK1 gene on malignant behavior of epithelial ovarian cancer cells and its related mechanism

  

  1. 1.Department of Obstetrics and Gynaecology, the Third Hospital of Hebei Medical University, 
    Shijiazhuang 050051, China; 2.The 7th Department of Obstetrics, Maternal and Child Health 
    Hospital, Hebei Province, Shijiazhuang 050006, China; 3.Department of Gynaecology, 
    the Eighth People′s Hospital of Hebei Province, Shijiazhuang 050024,  China

  • Online:2022-11-25 Published:2022-12-28

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Abstract: Objective To explore the effect of sphingosine kinase 1（SPHK1） on the biological behavior changes of ovarian cancer cells and its related mechanisms. Provide new evidence for the early diagnosis, prognosis, drug resistance treatment, and targeted therapy of epithelial ovarian cancer. 
Methods After transfecting SKOV3 cells with small interfering RNA (siRNA) to interfere with the expression of SPHK1 gene, we detected the effect of the changes of SPHK1 expression on cell proliferation, cell cloning and sensitivity to cisplatin, and also on the expression of stem cell markers NANOG, and the drug resistance-related gene MDR1. 
Results Decreased expression of SPHK1 gene caused a decrease in the proliferation of ovarian cancer cells, a decrease in clonogenicity, and an increase in sensitivity to cisplatin. The decreased expression of SPHK1 affected the expression of NANOG and MDR1 in ovarian cancer cells. 
Conclusion SPHK1 may influence the proportion of tumor stem cells through the NANOG, thereby affecting the malignant behavior of epithelial ovarian cancer. 


Key words: ovarian neoplasms, sphingosine, neoplastic stem cells