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Table of Content

    25 October 2021, Volume 42 Issue 10
    Observation on the effect of Sacubitril/valsartan in the treatment of patients with heart failure after acute myocardial infarction
    ZHANG Yang-chuan, FU Jin-juan, CAI Lin
    2021, 42(10):  1123-1127.  doi:10.3969/j.issn.1007-3205.2021.10.002
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    Objective To observe the effect of Sacubitril/valsartan, in comparison to benazepril, in the treatment of patients with heart failure after acute myocardial infarction(AMI), so as to improve the prognosis. 
    Methods A total of 86 patients with heart failure after AMI undergoing percutaneous coronary intervention(PCI) were selected. According to different treatment methods, they were divided into control group(benazepril) and observation group(Sacubitril/valsartan), with 43 cases in each group. At 3 months after operation, echocardiographic data[left ventricular end-systolic volume(LVESV), left ventricular end-diastolic volume(LVEDV), left ventricular ejection fraction(LVEF), stroke volume(SV)], serum inflammatory factors[high-sensitivity C-reactive protein(hs-CRP), interleukin-6(IL-6)], N-terminal pro-B-type natriuretic peptide(NT-proBNP), 6-min walking test, adverse reactions, prognosis and outcome were compared. 
    Results After treatment, LVESV and LVEDD in the two groups were lower than those before treatment, and LVEF and SV were higher than those before treatment. However, LVESV and LVEDD in the observation group were lower than those in the control group, and LVEF and SV were higher than those in the control group, with statistically significant differences(P<0.05). The levels of hs-CRP, IL-6 and NT-proBNP in the two groups were lower than those before treatment, which were lower in the observation group than in the control group, with statistically significant differences(P<0.05). There was no statistically significant difference in the incidence of adverse reactions, such as hypotension, hyperkalemia and renal insufficiency between the two groups(P>0.05). There was no statistically significant difference in recurrent myocardial infarction, ventricular tachycardia/frequent ventricular premature beats and sudden cardiac death between two groups(P>0.05). The re-hospitalization rate of patients with heart failure in the observation group was lower than that in the control group, and the 6-min walking test in the observation group was longer than that in the control group, with statistically significant differences(P<0.05). 
    Conclusion Compared with benazepril, Sacubitril/valsartan can improve left ventricular function and left ventricular remodeling in patients with heart failure after myocardial infarction, increase its activity tolerance, and reduce the rate of re-hospitalization for heart failure, but will not increase the incidence of adverse reactions and adverse cardiovascular events. 

    Construction and analysis of the competitive endogenous RNA network in amnestic mild cognitive impairment
    ZHANG Wei, HONG Bo, LIU An, YUAN Meng-ya, WANG Jing-hua, WANG Tao
    2021, 42(10):  1128-1132,1148.  doi:10.3969/j.issn.1007-3205.2021.10.003
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    Objective To investigate the correlation of non-coding RNA(ncRNA) dysfunction in plasma of patients with Alzheimer′s disease(AD) at the stage of amnestic mild cognitive impairment(aMCI) and the pathophysiology of AD and to explore the potential biomarkers and mechanisms underlying AD. 
    Methods Flve subjects with aMCI who met the golden standard for diagnosis and five normal controls(NC) were enrolled. Microarray analysis technology was used to analyze the expression profiles of long noncoding RNA(lncRNA), microRNA(miRNA) and messenger RNA(mRNA) in plasma of aMCI and NC. R software was used to screen out differentially expressed lncRNA, miRNA and mRNA, and PicTar 2005, TargetScan 5.1 and miRanda v5 database were used to detect the relationship between RNAs, to establish a competitive endogenous RNA(ceRNA) network. Gene Ontology(GO) functional enrichment and Kyoto Encyclopedia of Genes and Genomes(KEGG) pathway enrichment analysis were conducted for the differentially expressed mRNAs. 
    Results A total of 287 lncRNAs, 46 miRNAs and 208 mRNAs were differentially expressed in the peripheral plasma between aMCI and NC(P<0.05), in which 3 lncRNAs, 5 miRNAs and 7 mRNAs participated in the construction of ceRNA regulatory network. The results of GO and KEGG showed that differentially expressed mRNAs were mainly accumulated in biological processes such as RNA metabolism, cytoplasmic stress granules, transcriptional regulatory factor activities, herpes simplex infection pathway, oxytocin signaling pathway, and folic acid synthesis pathway. 
    Conclusion Different ncRNAs are involved in the pathogenesis of AD. The constructed ceRNA network helps to improve the understanding of the molecular biology of AD, and ncRNAs may become biomarkers for AD diagnosis and new targets for therapeutic intervention.

    Expression of FEER, NEU%, IL-6, RBC-C3bR in patients with Ⅲ and Ⅳ degree bedsore infection secondary to cerebral infarction and their value in predicting wound healing
    BAO Li-gai, DONG Qian
    2021, 42(10):  1133-1138.  doi:10.3969/j.issn.1007-3205.2021.10.004
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    Objective To investigate the expression of forming enhancement rosette rate(FEER), neutrophil %(NEU%), interleukin-6(IL-6), and erythrocyte C3b receptor wreath rate(RBC-C3bR) in patients with Ⅲ and Ⅳ degree bedsore infection secondary to cerebral infarction and their predictive value in wound healing. 
    Methods A total of 43 patients with Ⅲ degree bedsore infection secondary to cerebral infarction(Ⅲ degree group), 43 patients with Ⅳ degree bedsore infection secondary to cerebral infarction(Ⅳ degree group) and 43 healthy people(control group) were selected from Liangxiang Hospital of Fangshan District in Beijing. The general data, FEER, NEU%, IL-6, and RBC-C3bR levels of each group were compared, and Spearman correlation analysis was used to analyze the relationship between FEER, NEU%, IL-6, RBC-C3bR and the degree of bedsore. The FEER, NEU%, IL-6, RBC-C3bR levels and the Pressure Ulcer Scale for Healing(PUSH) scores of patients with healed and unhealed wound before treatment, at 10 d and 20 d after treatment were compared. Pearson analysis was used to analyze the correlation between FEER, NEU%, IL-6, RBC-C3bR and PUSH score, and receiver operating characteristic curve(ROC) and area under ROC(AUC) were used to analyze the value of each index in predicting wound healing. 
    Results FEER and RBC-C3bR were the lowest in Ⅳ degree group, followed by Ⅲ degree group and control group, while NEU% and IL-6 were the highest in Ⅳ degree group, followed by Ⅲ degree group and control group, suggesting significant difference between groups(P<0.05). FEER and RBC-C3bR were negatively correlated with the degree of bedsore, and NEU% and IL-6 were positively correlated with the degree of bedsore(P<0.05). At 10 d after treatment, the FEER and RBC-C3bR of the patients with healed wound showed an increasing trend, and the NEU% and IL-6 showed a decreasing trend; there were statistically significant differences between the indicators at 20 d after treatment and those at 10 d after treatment(P<0.05). The FEER and RBC-C3bR of patients with unhealed wounds at 10 d after treatment were higher than those before treatment, and NEU% and IL-6 were lower than those before treatment(P<0.05). However, there was no statistically significant difference between the indexes at 20 d after treatment and those at 10 d after treatment(P>0.05). The FEER and RBC-C3bR of patients with healed wound were higher than those of patients with unhealed wound at 10 d and 20 d after treatment, and the NEU% and IL-6 were lower than those of the unhealed(P<0.05). FEER and RBC-C3bR at 10 d and 20 d after treatment were negatively correlated with PUSH score, while NEU% and IL-6 were positively correlated with PUSH score(P<0.05), and the correlation at 10 d after treatment was stronger than that at 20 d after treatment. The AUC for predicting wound healing was the largest in IL-6(0.823), followed by NEU%(0.819), FEER(0.714) and RBC-C3bR(0.709), and the AUC of the combination of indicators was 0.896(P<0.05). 
    Conclusion The expressions of FEER and RBC-C3bR in patients with Ⅲ and Ⅳ degree bedsore infection secondary to cerebral infarction decreased significantly, and the expressions of NEU% and IL-6 increased significantly before treatment. After treatment, the increase of FEER, RBC-C3bR and the decrease of NEU% and IL-6 can indicate that the wound tends to heal. The detection of the level of each index at 10 d after treatment is expected to become a biomarker for predicting wound healing. 

    Analysis of the risk assessment value of miR-145, ABCD2 score combined with arterial ultrasound for the progression of cerebral infarction after TIA attack
    YU Lan, ZANG Han-qing, WEI Nai-lian
    2021, 42(10):  1139-1143.  doi:10.3969/j.issn.1007-3205.2021.10.005
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    Objective To explore the value of microRNA-145(miR-145), ABCD2 scores(age, blood pressure, clinical features, duration of symptoms, diabetes) combined with arterial ultrasound in the risk assessment of patients with transient ischemic attack(TIA) progressing to cerebral infarction(CI). 
    Methods A total of 132 TIA patients were selected from Department of Neurology of our hospital. According to presence or absence of progression to CI, patients were divided into non-TIA progression to CI group(non-progression group, n=76) and TIA progression to CI group(progression group, n=56). In addition, the two groups were all evaluated by miR-145, ABCD2 score and miR-145+abcd2+ artery ultrasound. ABCD2 score of the two groups was compared; the miR-145 was detected by PT-PCR, and fibrinogen(FIB), hypersensitive C-reactive protein(hs-CRP), and low-density lipoprootein cholesterol(LDL-C) were compared by enzyme-linked immunoabsorbent assay. The detection rate, missed diagnosis, misdiagnosis rate and diagnostic efficiency of the three methods were compared. 
    Results Compared with non-progression group, the ABCD2 score increased and the expression of miR-145 decreased in progression group(P<0.05); FIB and LDL-C levels decreased and hs-CRP levels increased(P<0.05). Compared with the detection of miR-145 and ABCD2 score, the detection rate of miR-145+ABCD2 score + arterial ultrasound increased in patients with TIA progressing to CI(P<0.05). Compared with the detection rate of miR-145 and ABCD2 score, the misdiagnosis rate and missed diagnosis rate of the miR-145+ABCD2 score+arterial ultrasound test decreased(P<0.05). The sensitivity, specificity and accuracy of the miR-145+ABCD2 score+arterial ultrasound test increased compared with the detection of the miR-145 and ABCD2 scores(P<0.05). 
    Conclusion The use of miR-145, ABCD2 score combined with arterial ultrasound to detect patients with CI after TIA attack has a high detection rate and sensitivity, and has a high value for risk assessment of CI after TIA attack, which is worthy of clinical popularization. 

    Clinical application of regional citrate anticoagulation in continuous renal replacement therapy in critically ill patients
    JIAO Qing-hai, ZHENG Li-huan, ZHANG Xue-mei, WANG Zheng, DU Fei, CHEN Liang
    2021, 42(10):  1144-1148.  doi:10.3969/j.issn.1007-3205.2021.10.006
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    Objective To investigate the efficacy and safety of regional citrate anticoagulation(RCA) in continuous venous hemofiltration(CVVH) in critically ill patients. 
    Methods A retrospective analysis of the general data of 668 critically ill patients who received CVVH treatment was performed.They were divided into the RCA group and unfractionated heparin(UFH) group based on anticoagulation methods. The acute physiology and chronic health(APACHEⅡ) score, causes of treatment with continuous renal replacement therapy(CRRT), filter life, reasons for suspension of hemofiltration, and complications between two groups were compared in both groups. 
    Results The filter life of RCA group was longer than that of UFH group(P<0.001). The proportion of filter life<24 h in UFH group was higher than that in RCA group, and the proportion of filter life ≥48 h in UFH group was lower than that in RCA group, with significant difference(P<0.05). There was no significant difference between two groups when the filter life was 24-48 h(P>0.05). The causes of suspension of hemofiltration in two groups were analyzed. The coagulation ratio of filter in RCA group was lower than that in UFH group, the proportion of getting off the machine at the end of treatment was higher than that in UFH group, and the proportion of replacing filter in RCA group was higher than that in UFH group after treatment for more than 72 h(P<0.05). There were no serious bleeding events in both groups. After treatment, there was no significant difference in serum total Ca2+, arterial blood pH, free Ca2+, Na+, HCO3- between two groups(P>0.05). 
    Conclusion Compared with UFH anticoagulation, RCA in critically ill patients undergoing CVVH can significantly prolong the filter life, reduce the application of supplies, and have no obvious metabolic complications or bleeding risk. It is expected to be the first-line anticoagulation method for CVVH treatment in ICU patients.

    Clinical efficacy of ulinastatin in the treatment of paraquat poisoning and its influence on inflammatory cytokines and T-lymphocyte subsets
    LIU Jia-yan, WANG Xin, LI Kang-ran, KONG Xiang-yu, QIN Li, CAO Zhi-jiao
    2021, 42(10):  1149-1154.  doi:10.3969/j.issn.1007-3205.2021.10.007
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    Objective To investigate the changes of interleukin-8(IL-8), tumor necrosis factor-α(TNF-α), matrix metalloproteinase-9(MMP-9) and T-lymphocyte subsets in plasma of patients with paraquat poisoning and the effect of ulinastatin on the above indicators, in order to study the curative effect of ulinastatin on paraquat poisoning and provide a new method for the treatment of paraquat. 
    Methods A total of 80 patients meeting the criteria of acute paraquat poisoning were selected and divided into two groups: poisoning group(n=40) and treatment group(n=40). The patients in the poisoning group received conventional treatment, while the other group received ulinastatin in addition to the basic treatment. The clinical indicators of the two groups were observed at 14 d after treatment, and the blood samples were collected at 7 d and 14 d after treatment. The levels of IL-8, TNF-α and MMP-9 in plasma were detected. The levels of CD3+, CD4+, CD8+ and the ratio of CD4+/CD8+ in peripheral blood were detected by SAP enzyme labeling method. 
    Results The incidence of acute respiratory distress syndrome(ARDS), survival rate, duration of ICU stay and survival time of dead patients in the treatment group were better than those in the poisoning group(P<0.05). Compared with the poisoning group, the levels of IL-8, TNF-α, MMP-9 and CD8+ in the treatment group were significantly decreased(P<0.05), while CD3+, CD4+, and CD4+/CD8+ were significantly increased, suggesting significant difference(P<0.05). 
    Conclusion Ulinastatin can improve the clinical efficacy of patients wiht paraquat poisoning, and its underlying mechanism may be related to its inhibition of the release of inflammatory factors and regulation of immune function of T-lymphocyte subsets, with obvious effect. Therefore, it is worthy of clinical promotion.

    Effect of perioperative systematic management under the concept of ERAS on postoperative recovery and short-term prognosis of patients with intracranial meningioma
    YANG Hui-ting, ZHAO Bin-fang, WANG Liang, CAO Ya-ni
    2021, 42(10):  1155-1160.  doi:10.3969/j.issn.1007-3205.2021.10.008
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    Objective To investigate the impact of perioperative systematic management under the concept of enhanced recovery after surgery(ERAS) on postoperative recovery and short-term prognosis of patients with intracranial meningioma. 
    Methods The clinical data of 113 patients with intracranial meningioma treated in our hospital were collected for a retrospective cohort study. According to different nursing programs, they were divided into the control group(n=56) and the observation group(n=57). The control group was treated with routine nursing and the observation group was supplementd with perioperative systemic management under the concept of ERAS. The postoperative recovery(duration of ICU stay, length of hospitalization, the first time of off-bed activity, time of first defecation), recent prognosis, incidence of complications and nursing satisfaction in the two groups were calculated. Post-traumatic stress disorder self-rating scale(PTSD-SS) and medical coping style questionnaires(MCMQ) before and after nursing were compared. 
    Results The duration of ICU stay, length of hospitalization, the first time of off-bed activity and first defecation time were shorter than those in the control group(P<0.05). The scores of the five dimensions of the PTSD-SS scale in the observation group after intervention were lower than those of the control group(P<0.05). The avoidance and yield scores of the observation group after intervention were lower than those of the control group, and the face score was higher than that of the control group(P<0.05). At 1 month, 2 months, and 3 months after operation, the KPS score of the observation group was higher than that of the control group(P<0.05). The incidence of complication in the observation group was lower than that of the control group, while the nursing satisfaction was higher than that of the control group(P<0.05). 
    Conclusion Perioperative systemic management under the concept of ERAS can be applied to patients with intracranial meningioma, which can promote postoperative recovery, reduce post-traumatic stress disorder, improve patients′ coping style and prognosis, and play a positive role in reducing the incidence of complications and improving nursing satisfaction of the patients. 

    Effect of different dermal substitute materials combined with vacuum sealing drainage technique on repair of tendon-exposed wound in diabetic patients
    NIU Da-wei, GUO Peng-fei, HAN Zhi-xin
    2021, 42(10):  1161-1165.  doi:10.3969/j.issn.1007-3205.2021.10.009
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    Objective To investigate the effect of dermal substitute materials combined with vacuum sealing drainage(VSD) technique on the repair of tendon-exposed wound in patients with diabetes mellitus(DM). 
    Methods The clinical data of 8 DM patients with tendon-exposed wound that were treated with acellular dermal matrix(ADM) in People′s Hospital of Anyang City were collected and they were enrolled as ADM group. The clinical data of 12 DM patients with tendon-exposed wound treated with artificial dermis(AD) in the First Affiliated Hospital of Zhengzhou University for the treatment of tendon-exposed wound were collected and they were enrolled as AD group. After wound debridement, the exposed tendon was covered with AD or ADM, and the second stage of autologous blade thickness skin graft was performed, combined with the application of closed negative pressure drainage technology. The times of skin grafting, the interval of transplantation, the survival of skin graft at 7 d after skin grafting, hospitalization cost, the incidence of postoperative complications and the recurrence of ulceration in the operation area at 3 months of follow-up were observed. 
    Results There was no significant difference in the number of skin grafting, survival rate of skin graft, postoperative complications and postoperative follow-up between two groups(P>0.05). The average transplantation interval in ADM group was less than that in AD group, and the average hospitalization cost was higher than that in AD group(P<0.05). 
    Conclusion Dermal substitute materials combined with vacuum sealing drainage technique is an effective avenue to repair the wounds of tendon-exposed wound. ADM and AD have their own advantages and disadvantages, which should be selected according to the actual condition.

    Early repair effect of double-layer artificial dermis grafting on deep burn wounds of joints
    HE Jing, XU Gang, JIANG Hai, LIU Yu, LIU Dong-mei
    2021, 42(10):  1166-1170.  doi:10.3969/j.issn.1007-3205.2021.10.010
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    Objective To explore the effect of early application of double-layer artificial dermis in the treatment of deep burn wounds of joints. 
    Methods A total of 40 patients with severe and deep burn wounds of joints were selected as research subjects, and randomly divided into the observation group(n=20) and the control group(n=20) according to different treatment methods. In the observation group, the deep burn wounds of joints were treated with escharectomy or tangential excision at 3-5 d after injury, and then the wound were covered with Lando double-layer artificial dermis. After good vascularization of the artificial dermis, the silica gel layer was removed and the autologous split-thickness skin was grafted. In the control group, escharectomy or tangential excision was performed on deep burn wounds of joints at 3-5 d after injury, and then the thin split-thickness autograft was performed. The survival rate of skin graft at 7 d after implantation and the healing rate of wound surface at 21 d after implantation were recorded. After wound healing, the rehabilitation training was guided, the data on the incidence of joint blister, pain and pruritus were collected, and the quality of life of patients who suffered joint scar hyperplasia and burn was evaluated.  
    Results The survival rate of skin grafts in the observation group was higher than that in the control group at dressing change at 7 d after skin graft surgery. The wound healing rate in the observation group was higher than that in the control group at 21 d after surgery. The incidence of blisters, pain, and pruritus in the observation group was lower than that in the control group, with statistical significance(P<0.05). After 12 months of follow-up, according to the Vancouver Scar Scale score, the observation group had better scar hyperplasia than the control group. According to the BSHS-B score, the observation group had better quality of life after burn than the control group, with statistical significance(P<0.05). 
    Conclusion Double-layer artificial dermis can be safely applied to the early treatment of deep burn wounds of joints in patients, which has a high survival rate of skin grafts, low incidence of adverse wound events, insignificant scar hyperplasia at joints, and high quality of life for burn patients. 

    Preventive effect of LTH combined with round ligament suspension on postoperative PFD in patients with uterine leiomyoma
    XIU Chen, LI Juan, WANG Zhuo, NIU Cui-li, CHEN Xiao-lin, LIU Xing-qiao
    2021, 42(10):  1171-1174,1184.  doi:10.3969/j.issn.1007-3205.2021.10.011
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    Objective To analyze the preventive effect of laparoscopic total hysterectomy(LTH) combined with round ligament suspension on postoperative pelvic floor dysfunction(PFD) in patients with uterine leiomyoma. 
    Methods A total of 112 patients with uterine leiomyoma admitted to our hospital were divided into observation group(n=56) and control group(n=56) according to the random number table method. Patients in both groups were treated with LTH, and the observation group was combined with round ligament suspension. The PFD was compared in the two groups after different treatment. According to presence of PFD, the patients in the two groups were divided into the occurrence group and the non-occurrence group. The clinical records of the two groups were compared, and the risk factors of postoperative PFD were analyzed by Logistic regression model. 
    Results After 6 months of follow-up, there were 38 patients with PFD. The incidence of frequent urination and stress urinary incontinence in the observation group was significantly lower than that in the control group(P<0.05). The hospitalization days, exhaust time and PFIQ-7 score of the patients in the observation group were significantly lower than those in the control group(P<0.05). Logistic regression analysis showed that diabetes(OR=2.208, 95%CI:1.457-3.345), number of pregnancies(OR=1.592, 95%CI:1.059-2.393), diameter of uterine leiomyoma(OR=2.545, 95%CI:1.076-6.016) and age(OR=2.358, 95%CI:1.275-4.364) were the risk factors for postoperative PFD(P<0.05). 
    Conclusion The risk factors of PFD in patients with uterine leiomyoma after LTH were diabetes mellitus, number of pregnancies and delivery, diameter of uterine leiomyoma and age type. LTH combined with round ligament suspension can reduce the risk of postoperative PFD.

    Clinical significance of circulating tumor cell typing as a new generation of tumor markers in the diagnosis of cervical cancer
    ZHANG Jun-hua, YUAN Gao-liang, JI Li-wei
    2021, 42(10):  1175-1179.  doi:10.3969/j.issn.1007-3205.2021.10.012
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    Objective To explore the distribution of circulating tumor cell(CTC) subtypes and circulating tumor microemboli(CTM) in peripheral blood of patients with cervical cancer(CC), and to analyze the correlation between CTC/CTM and the expression levels of tumor markers SCC-Ag, CA125 and CA199. 
    Methods The elbow venous blood was collected from 32 patients with initially diagnosed CC,the subtraction enrichment-immunofluorescence staining-fluorescence in situ hybridization(SE-iFISH) was employed to detect CTC/CTM, and the electro-chemiluminescence immunoassay was used to measure the serum levels of SCC-Ag, CA125 and CA199. The correlation between CTC/CTM and pathological features and serum tumor markers was analyzed. 
    Results Among 32 cases within itially diagnosed CC, 29 cases were positive for CTC(positive rate of 90.6%), and 9 cases were positive for CTM(positive rate of 28.1%). There was a significant difference in the positive rate of CTC in CC patients with different lymph node metastasis(P<0.05). There was a significant difference in the positive rate of CTM among CC patients with different clinical stages and lymph node metastasis(P<0.05). In addition, 28 cases were positive for CA125(positive rate of 87.5%), 28 cases were positive for CA199(positive rate of 87.5%), and 24 cases were positive for SCC-Ag(positive rate of 75.0%). There was no correlation of CTC and CTM with the serum levels of CA125, CA199 and SCC-Ag(P>0.05). 
    Conclusion CTC/CTM monitoring can be used as an important indicator of the progression of CC.

    Clinical effect of salmeterol xinafoate and fluticasone propionate aerosol combined with sublingual immunotherapy with dust mite drops on dust mite allergic asthma
    ZHENG Fang, BIAN Xiang-li, LI Miao-chen, WU Zhi-min
    2021, 42(10):  1180-1184.  doi:10.3969/j.issn.1007-3205.2021.10.013
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    Objective To observe and summarize the clinical effect of salmeterol xinafoate and fluticasone propionate aerosol combined with sublingual dust mite drops in children with dust mite allergic asthma.  
    Methods A total of 85 children with dust mite allergic asthma who were admitted to our hospital were selected and divided into 2 groups by random number table method. The control group received salmeterol xinafoate and fluticasone propionate aerosol(n=42) and the observation group received salmeterol xinafoate and fluticasone propionate aerosol combined with sublingual immunotherapy with dust mite drops(n=43). The therapeutic effects of the two groups and the changes in clinical indicators before and after treatment were summarized, and incidence of adverse reactions during treatment was analyzed in two groups. 
    Results After treatment, the total effective rate in the observation group was 93.02%(40/43), which was significantly higher than 76.19%(32/42) in the control group, and the difference was statistically significant(P<0.05). After treatment, the improvement of serum dust mite specific immunoglobulin G(SIgG4) and serum interleukin(IL)-12 in the observation group was better than that in the control group, and the difference was statistically significant(P<0.05). The daytime symptom score and nighttime symptom score of asthma in the observation group were lower than those in the control group after treatment, and the difference was statistically significant(P<0.05). During the treatment period, there was mild throat pain in both groups, including 4 cases(9.30%) in the observation group and 3 cases(7.14%) in the control group, however, there was no statistically significant difference(χ2=0.131, P=0.717).  
    Conclusion Salmeterol xinafoate and fluticasone propionate aerosol combined with sublingual immunotherapy with dust mite drops for children with dust mite allergic asthma is effective in ameliorating the asthma symptoms of children such as airway inflammation and immune imbalance. Therefore, it has good application value in clinical practice.

    Analysis of hearing monitoring results of physical checkup of workers exposed to noise in Rongchang District, Chongqing
    LI Hai-tao, WANG Wei-hong, CHEN Shu-jin, ZHAO Xiao-yan
    2021, 42(10):  1185-1188.  doi:10.3969/j.issn.1007-3205.2021.10.014
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    Objective To analyze the hearing monitoring results of physical checkup and influencing factors of noise-exposed workers in Rongchang District, Chongqing. 
    Methods A total of 1 339 noise-exposed workers for hearing monitoring were selected for the study. All survey subjects underwent pure tone hearing threshold examination, and the factors that might affect hearing, such as gender and age, of patients were collected, to analyze the influencing factors of the hearing loss of noise-exposed workers in Rongchang District, Chongqing. 
    Results Among 1 339 patients in this study, 583(43.54%) had hearing loss. There were significant differences between the hearing loss group and the non-hearing loss group with respect to gender, age, body mass index(BMI), length of service in noise work, educational level, wearing protective earplugs, smoking history, drinking history(P<0.05). The results of multivariate Logistic regression analysis showed that male, age of 51-60 years, BMI≥25, length of service in noise work for 21-30 years, education level of junior middle school and below, no wearing protective earplugs, drinking history were the risk factors of hearing loss of noise-exposed workers in Rongchang District of Chongqing(P<0.05). 
    Conclusion The hearing loss of noise-exposed workers in Rongchang District of Chongqing is a prominent symptom. Gender, age, BMI, noise power, educational level, wearing protective earplugs, drinking history are the influencing factors of hearing loss of noise-exposed workers. Intervention of the above factors can protect the hearing of noise-exposed workers.

    Clinical observation of Adapalene gel-assisted supramolecular salicylic acid and human-like collagen dressing in the treatment of patients with mild to moderate acne
    LIU Xiao-li
    2021, 42(10):  1189-1193,1209.  doi:10.3969/j.issn.1007-3205.2021.10.015
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    Objective To investigate the effect of adapalene gel-assisted supramolecular salicylic acid and human-like collagen dressings in the treatment of patients with mild to moderate acne and its influence on skin barrier function and the cosmetic satisfaction. 
    Methods A total of 123 patients with mild to moderate acne admitted to our hospital were selected and divided into three groups according to the random number table method, with 41 cases in each group. The control group A was treated with supramolecular salicylic acid and human-like collagen dressing, the control group B was treated with adapalene gel, and the observation group was treated with adapalene gel-assisted supramolecular salicylic acid and human-like collagen dressing. The therapeutic effect, time of symptom improvement, skin lesions, and skin barrier function indexes[transepidermal water loss(TEWL), stratum corneum water content, pH value] before and after treatment, the cosmetic satisfaction and adverse reactions were compared among three groups. 
    Results The total effective rate of the observation group was higher than that of control group A and control group B(P<0.05). There was no significant difference between control group A and control group B(P>0.05). The duration of erythema, pain, scab formation and complete shedding of scab in the observation group were shorter than those in control group A and control group B, and the above indicators in control group A were shorter than those in control group B(P<0.05). At 4 weeks after treatment, the number of papules, acne, abscess, nodules and cysts in the three groups was less than that before treatment, and the number was less in observation group than in the control group A and control group B, and less in the control group A than in the control group B(P<0.05). The TEWL, stratum corneum water content and pH value in the three groups were better than those before treatment, and the above indicators were better in the observation group than in control group A and control group B, and better in the control group A than in control group B(P<0.05). The cosmetic satisfaction of the observation group was higher than that of control group A and control group B(P<0.05), while there was no significant difference between control group A and control group B(P>0.05). There was no significant difference in the incidence of adverse reactions among the three groups(P>0.05). 
    Conclusion Adapalene gel-assisted supramolecular salicylic acid and human-like collagen dressings are effective in treating mild to moderate acne, with good patient satisfaction and safety. 

    Ultrasound guidance combined with botulinum toxin type A in the treatment of post-stroke upper limb spasticity
    XIA Xue, XUE Mei, YANG Chun-yan, MA Jiang, YAO Xue-jing
    2021, 42(10):  1194-1197.  doi:10.3969/j.issn.1007-3205.2021.10.016
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    Objective To investigate the curative effect of ultrasound guidance combined with botulinum toxin type A(BTX-A) injection on post-stroke upper limb spasticity. 
    Methods Sixty stroke patients were randomly divided into two groups, with 30 cases in each group. The control group received conventional rehabilitation therapy, while the observation group received ultrasound guidance combined with BTX-A injection. Therapeutic effects of the two groups were compared. 
    Results At 4 weeks after treatment, the modified Ashworth scale(MAS) scores, clinic spasticity index(CSI) scores, Fugl-Meyer assessment scale(FMA) scores, initiative joint range of motion(AROM), modulus of rigidity(G), muscular thickness(MT) and fiber length(FL) of the observation group were better than those of the control group(P<0.05). 
    Conclusion Ultrasound guidance combined with BTX-A injection can significantly relieve upper limb spasticity, which, therefore, is worthy of clinical popularization.

    Comparative study between free-breathing non-contrast-enhanced 3D-TFE-WHequence coronary angiography and CTA
    LI Min, QIN Hong-tao, YANG Li, WANG Qi, DANG Meng-ting, WANG Xiang-ming
    2021, 42(10):  1198-1202.  doi:10.3969/j.issn.1007-3205.2021.10.017
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    Objective To evaluate the image quality of free-breathing non-contrast-enhanced 3D-TFE-WH sequences in different segments of coronary artery and their ability to display lumen stenosis. 
    Methods Ten volunteers were recruited as the research subjects and underwent coronary magnetic resonance angiography(MRA) and CT angiography(CTA) on the same day. The image quality of MRA and CTA was evaluated, and the ability of coronary MRA to display lumen stenosis was evaluated with coronary CTA as the standard. 
    Results There were 90 segments of coronary arteries in 10 volunteers, of which 83 segments(92.2%) met the diagnostic requirements by coronary MRA and 90 segments(100.0%) by CT. There was no significant difference in image quality of proximal and mid segment blood vessels between two methods(P>0.05). The image quality score of MRA in the distal segment of left anterior descending branch, left circumflex branch and right coronary artery was lower than that of CTA(P<0.05). MRA showed a total of 6 segments of lumen stenosis, including 2 segments of slight stenosis and 4 segments of mild stenosis. CTA showed 8 segments of lumen stenosis, including 6 segments of slight stenosis and 2 segments of mild stenosis. Taking CTA as the standard, MRA overestimated stenosis in 4 segments and underestimated stenosis in 5 segments. Two sections of CTA showed slight stenosis, which could not be evaluated by MR. The consistency of the two examination methods in the ability of lumen stenosis was medium(Kappa value=0.656). 
    Conclusion The image quality of free-breathingnon-contrast-enhanced 3D-TFE-WH sequence in proximal and mid segment of coronary artery and its ability to display non-mild lumen stenosis are similar to CTA. This technique can be used as a method for coronary artery disease screening in people with contraindication of contrast agents.

    Analysis on the occurrence and influencing factors of pressure injury in gynecological lithotomy
    TANG Yan-wei
    2021, 42(10):  1203-1209.  doi:10.3969/j.issn.1007-3205.2021.10.018
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    Objective To investigate the occurrence and influencing factors of pressure injury(PI) in gynecological lithotomy, and to provide scientific basis for reducing the risk of PI. 
    Methods A total of 613 patients undergoing gynecological lithotomy were prospectively divided into occurrence group and non-occurrence group according to presence or absence of PI. The location and stage distribution of PI in gynecological lithotomy were calculated. The age, body mass index(BMI), anemia, hypoproteinemia, preoperative anxiety, duration of operation, leakage of urine, hypothermia during operation, history of PI, use of vasoactive drugs, intraoperative sweating, diabetes, hypertension, chronic liver disease, hyperlipidemia, and heart disease were compared between the two groups. Multivariate Logistic regression equation was used to analyze the influencing factors of PI in gynecological lithotomy, and the predictive value of Braden score and Waterlow scale score on PI in gynecological lithotomy was analyed. 
    Results The incidence of PI in 613 patients undergoing gynecological lithotomy was 7.99%(49/613). The high to low sites were sacrococcygeal(42.86%), sciatic(18.37%), leg support contact(14.29%), scapula(10.20%), posterior part of head(8.16%) and restraint belt(6.12%); Stage Ⅲ(32.65%) was the most common stage of PI; Stage Ⅲ and Ⅳ PI in sacrococcygeal and sciatic sites were more common. There were significant differences in age, BMI, anemia, hypoproteinemia, preoperative anxiety, duration of operation, leakage of urine, hypothermia, PI history, use of vasoactive drugs, intraoperative sweating, diabetes, hypertension and chronic liver disease in occurrence group and non-occurrence group(P<0.05). There was no significant difference in the distribution of hyperlipidemia and heart disease between the occurrence group and the non-occurrence group(P>0.05). Multivariate Logistic regression analysis showed that age>70 years, being lean, hypoproteinemia, duration of operation ≥2 h, hypothermia during operation, history of PI, use of vasoactive drugs, intraoperative sweating, diabetes combined with chronic liver disease were the influencing factors of PI in women undergoing lithotomy(P<0.05). The preoperative Braden score of the occurrence group was lower than that of the non-occurrence group, while the score of Waterlow scale was higher than that of the non-occurrence group(P<0.05). The sensitivity and specificity of Braden score in predicting PI after gynecological lithotomy were 63.27% and 86.70%, respectively. The sensitivity and specificity of Waterlow scale in predicting PI after gynecological lithotomy were 57.14% and 88.30%, respectively. The sensitivity and specificity of Braden score + Waterlow scale in predicting PI after gynecological lithotomy were 71.43% and 93.09%, respectively. The AUC of Braden score+Waterlow scale score in predicting PI after gynecological lithotomy was greater than that of single Braden score and Waterlow scale score(P<0.05). 
    Conclusion There are different degrees of PI risk in gynecologic lithotomy. Age>70 years, being lean, hypoproteinemia, duration of operation ≥2 h, leakage of urine, hypothermia during operation, history of PI, use of vasoactive drugs, intraoperative sweating, diabetes combined with chronic liver disease are the influencing factors of PI. Braden score and Waterlow scale can effectively predict the risk of PI. It is recommended to take preventive measures against the above factors to reduce the incidence of PI during gynecological lithotomy.

    Dosimetric advantages of bonemarrow-sparing intensity modulated radiotherapy for cervical cancer after hysterectomy
    TIAN Lei, XUE Xiao-ying, WANG Yan-qiang, XIAO Zhi-qing, GE Xiao-hui
    2021, 42(10):  1210-1214.  doi:10.3969/j.issn.1007-3205.2021.10.019
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    Objective The dosimetric parameters of bone marrow sparing-intensity modulated radiation therapy(BMS-IMRT) and conventional intensity modulated radiation therapy(IMRT) in patients with cervical cancer after hysterectomy were compared and analyzed, providing a theoretical basis for the clinical application of BMS-IMRT. 
    Methods Ten patients with cervical cancer undergoing hysterectomy were selected to design BMS-IMRT and IMRT plans respectively, and the dosimetric indexes of the planning target volume(PTV) and organs at risk(OARs) were statistically analyzed. 
    Results The mean value of Dmax and Dmean in BMS-IMRT group were higher than those in IMRT group and the homogeneity index(HI) of PTV in the BMS-IMRT group was inferior to that in IMRT group(P<0.05). The mean V10, V20, V30, V40 of bone marrow in BMS-IMRT group were lower than those in IMRT group, and the Dmax of femoral heads was also lower in the BMS-IMRT group than in IMRT group(P<0.05). The radiation dose of rectum and bladder in the two plans was not statistically significant(P>0.05). 
    Conclusion BMS-IMRT can reduce the radiation dose of bone marrow while ensuring the coverage of PTV compared with the conventional IMRT in postoperative patients with cervical cancer and dose not increase the radiation dose of the rectum and bladder, theoretically reducing the occurrence of hematological toxicity.