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    25 February 2025, Volume 46 Issue 2
    Analysis of risk factors for recurrence of adjacent vertebral fracture following percutaneous vertebroplasty in the treatment of osteoporotic vertebral compression fractures
    HOU Li-yuan1, ZHANG Jun-zhe2, SHI Sha1, ZHANG Wei1
    2025, 46(2):  131-136.  doi:10.3969/j.issn.1007-3205.2025.02.002
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    Objective To investigate the risk factors associated with the recurrence of adjacent vertebral fractures following percutaneous vertebroplasty (PVP) in the treatment of osteoporotic vertebral compression fractures (OVCF). 
    Methods A total of 312 patients who underwent unilateral PVP surgery in the Third Hospital of Hebei Medical University were selected and divided into the normal group (n=269) and the vertebral refracture group (n=43) based on the recurrence of fractures postoperatively. Surgical and postoperative indicators of both groups were statistically analyzed; Postoperative indicators included gender, age, height, weight, residence (rural/urban), smoking, alcohol consumption, diabetes, hypertension, cardiovascular disease, and body mass index (BMI), and related surgical indicators included duration of operation, volume of bone cement injection, bone mineral density (BMD), postoperative vertebral compression rate, bone cement leakage, and postoperative Cobb angle. Continuous variables between the two groups were compared using t or U tests, and categorical variables were analyzed using chi-square tests. Predictors with significant differences in univariate analysis (P<0.05) were included in multivariate Logistic regression analysis. Receiver operating characteristic (ROC) curves were employed to determine the optimal cutoff values for statistically significant continuous variables (P<0.05). 
    Results During the 6-month follow-up period, 43 patients experienced refractures postoperatively, resulting in an incidence rate of 13.78%. Bone cement leakage occurred in 31 patients, with an incidence rate of 9.94%. Multivariate Logistic regression analysis indicated that BMD < -3.4 g/cm2 (OR=6.638, P=0.008), bone cement injection volume > 5.6 mL (OR=19.178, P<0.001), postoperative Cobb angle > 14 ° (OR=18.970, P<0.001), and bone cement leakage (OR=10.715, P=0.002) were associated with the recurrence of adjacent vertebral compression and were independent risk factors. The Hosmer-Lemeshow test demonstrated good model fit (χ2=4.07, P=0.254). 
    Conclusion The incidence rate of vertebral refracture after PVP is 13.78%. BMD < -3.4 g/cm2, bone cement injection volume > 5.6 mL, postoperative Cobb angle >14 °, and bone cement leakage are independent risk factors for vertebral re-compression. Attention to the critical threshold of bone cement injection volume during surgery, reduction of the postoperative Cobb angle, and systematic anti-osteoporosis treatment can significantly decrease the incidence of refractures following vertebroplasty. 

    Meta-analysis of external fixator and plating internal fixation for distal radius fracture
    ZHANG Yu-heng1, ZHAO Yue-chun2, CHENG Yong-zhong1, SANG Zhi-cheng1
    2025, 46(2):  137-147.  doi:10.3969/j.issn.1007-3205.2025.02.003
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    Objective To evaluate the efficacy of external fixation after closed reduction and internal fixation with steel plate after open reduction in the treatment of distal radius fracture (DRF) using a systematic review. 
    Methods We retrieved publicly available databases, such as Pubmed, CNKI, and Wanfang, and to collect randomized controlled trail (RCT) about these two kinds of public tables from the inception of the database to July 2023. The retrieved literature was screened manually, and for the final included studies, Cochrane risk bias assessment tool was used to assess the quality of bias risk, and RevMan 5.4 software was used for Meta-analysis. The advantages and disadvantages of the two postoperative imaging, functional scores and complications were compared. 
    Results A total of 1 845 patients were included in 20 RCT literatures, including 928 patients in external fixator group and 917 patients in internal fixation with steel plate group. Meta-analysis results showed that there were significant differences inthe two treatment methods with respect to the ulnar deviation angle (MD=-0.72, 95%CI: -1.35--0.10, P=0.02) of the two treatments, ulna variation (MD=0. 80, 95%CI: 0.59-1.01, P<0.001), pronation (MD=-2.60, 95%CI: -4.88--0.31, P=0.03), visual analog scale (VAS) score (MD=0.10, 95%CI: 0.02- 0.17, P=0.01), and the infection rate (RR=5. 18, 95%CI: 2.50-10.73, P<0.001), and the internal fixation group was better than the external fixation group. However, there were no significant differences with respect to the palmar inclination angle (MD=0.35, 95%CI: -3.40-4.11, P=0.85), height of radius (MD=-0.50, 95%CI: -1.26-0.27, P=0.21), grip strength (MD=-0.15, 95%CI: -0.51-0.20, P=0.40), dorsiflexion (MD=-3.84, 95%CI: -9.60-1.91, P=0.19),palmar flexion (MD=-2.12, 95%CI: -6.47-2.24, P=0.34), supination (MD=-3.22, 95%CI: -6.46-0.02, P=0.05), radial deviation (MD=-0.01, 95%CI: -0.82-0.85, P=0.98), ulnar deviation (MD=-0. 00, 95%CI: -0.33-0.33, P=0.98), the disabilities of the arm, shoulder, and hand (DASH) score (MD=0.07, 95%CI: -3.02-3.16, P=0.96), the total incidence of complications (RR=1.10, 95%CI: 0.94-1.27, P=0.23), and the incidence of complex regional pain syndrome (RR=1.70, 95%CI: 0.96-3.03, P=0.07). 
    Conclusion Based on the current data, the open reduction and internal fixation with steel plate group is superior to the external fixation group in the recovery of ulnar deviation angle, ulnar variation, pronation movement and infection rate, but there is no significant difference between the two groups in palm inclination angle, reduction of radial height, recovery of grip strength, range of motion such as radial and ulnar deviation or supination, DASH score and total complication rate. 

    Clinical characteristics and influencing factors of phenomenon of comorbidity of rheumatoid arthritis and osteoarthritis in female patients
    WEN Xing-jun1, YANG Zhou2
    2025, 46(2):  148-153.  doi:10.3969/j.issn.1007-3205.2025.02.004
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    Objective To analyze the clinical characteristics and influencing factors of the phenomenon of comorbidity of rheumatoid arthritis (RA) and osteoarthritis (OA) in women, so as to provide a basis for the prevention and treatment of OA and the management of comorbidity patients. 
    Methods Detailed detailed medical records of 122 RA patients with OA treated at School of Clinical Medicine, Hainan Medical University/Hainan General Hospital were collected, and they were included in the case group. In the meantime, 70 simple RA patients were selected and included in the control group. The differences in clinical characteristics of the two groups were recorded and compared, and the influencing factors of comorbidity of RA and OA in females were further analyzed by multivariate Logistic regression analysis. A nomogram model was constructed according to the influencing factors of comorbidity of RA and OA. Bootstrap internal verification method and receiver operating characteristic (ROC) curve were used to verify the differentiation and clinical practicability of the model. 
    Results Compared with the control group, the case group had a longer course of simple RA, higher body mass index (BMI), a higher proportion of physical labor and menopausal, higher levels of C-reactive protein (CRP), and lower levels of 25-hydroxyvitamin D3 [25 (OH)D3], with a significant difference (P<0.05). Results of multivariate Logistic regression analysis showed that a long course of simple RA (95%CI: 1.044-1.350), high BMI (95%CI: 1.392-2.221), physical labor (95%CI: 1.040-9.182), menopausal (95%CI: 1.164-7.123), and high CRP levels (95%CI: 1.382-1.961) were risk factors for comorbidity of RA and OA in females, while high levels of 25 (OH) D3 (95%CI: 0.846-0.983) were protective factors for comorbidity of RA and OA in females (P<0.05). Based on the above influencing factors, a nomogram model of comorbidity RA and OA in females was constructed. Bootstrap internal verification showed that the standard curve of the model was close to the Y-X straight line, with a consistency index of 0.922, indicating good discrimination of the model. The ROC curve was drawn, and the results showed that the area under the ROC curve for predicting comorbidity of RA and OA in females was 0.922, 95%CI: 0.883-0.961, and P<0.001. 
    Conclusion Female patients with comorbidity of RA and OA have a long course of simple RA, high BMI, a high proportion of physical labor and menopausal, high levels of CRP, and low levels of 25 (OH) D3. These characteristics are also important factors affecting comorbidity of RA and OA. A nomogram model constructed based on these influencing factors has good predictive value for comorbidity of RA and OA. 

    Effect of expression of lncRNA VPS9D1-AS1 in bladder cancer on clinical manifestations and malignant biological behavior of bladder cancer
    LIU Zong-hang, QI Pan, LI Si-jie, DUAN Ya-tao, ZHANG Ai-li
    2025, 46(2):  159-165.  doi:10.3969/j.issn.1007-3205.2025.02.006
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    Objective To investigate the effect of the expression of long noncoding RNA (lncRNA) VPS9D1-AS1 in bladder cancer on the clinical manifestations and malignant biological behavior of bladder cancer. 
    Methods In total, 69 samples of cancer tissue and normal bladder epithelium were collected from patients with bladder cancer, who were treated in the Department of Urology, the Fourth Hospital of Hebei Medical University. The expression of VPS9D1-AS1 in bladder cancer tissue was detected by quantitative real-time polymerase chain reaction (qPCR), and its correlation with gender, age, smoking, hypertension, pathological grades, T stages, lymph node metastasis and distant metastasis was analyzed. The impacts of knockdown and overexpression of VPS9D1-AS1 on proliferation, migration and invasion ability of bladder cancer cells in vitro were investigated by cell proliferation assay, colony-forming assay, wound healing assay and Transwell invasion assay. 
    Results The expression of VPS9D1-AS1 in the bladder cancer tissue was significantly higher than that in the normal bladder epithelium (P<0.01). The expression of VPS9D1-AS1 in the high-grade urothelial carcinoma group was significantly higher than that in the low-grade urothelial carcinoma group (P<0.01). Knockdown and overexpression of VPS9D1-AS1 could inhibit and enhance the proliferation, migration and invasion ability of bladder cancer cell lines, respectively. 
    Conclusion LncRNA VPS9D1-AS1 increases the malignancy of bladder cancer cells and enhances the malignant biological behavior of bladder cancer cells. 

    The application value of the "Trinity Method" in the diagnosis of atypical renal tuberculosis
    LI Chong-bin, YIN Hang, FAN Zheng-chao, LIU Jian-zhen, HUANG Tian-hao
    2025, 46(2):  166-171.  doi:10.3969/j.issn.1007-3205.2025.02.007
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    Objective To discuss the application value of the "Trinity Method", which combines spiral computed tomography (CT) plain scan, T cell spot detection of tuberculosis infection (T-SPOT.TB) and rifampicin resistance real-time fluorescence quantitative nucleic acid amplification (GeneXpert MTB/RIF), in the diagnosis of atypical renal tuberculosis.  
    Methods In total, 75 patients with atypical renal tuberculosis diagnosed by Mycobacterium tuberculosis culture or postoperative pathological diagnosis and treated in the Department of Urology, Hebei Chest Hospital were enrolled as the tuberculosis group, and another 75 patients with non-tuberculous nephropathy were enrolled as the control group. The sensitivity, specificity, accuracy and area under curve (AUC) of different detection methods in the diagnosis of atypical renal tuberculosis were compared, with the final clinical diagnosis as the gold standard. 
    Results ① The sensitivity of CT, T-SPOT.TB, CT combined with T-SPOT.TB, and GeneXpert MTB/RIF was higher than that of Mycobacterium tuberculosis culture (84.00%, 100.00%, 84.00%, 62.67%, 9.33%, respectively). The sensitivity of CT combined with T-SPOT.TB was lower than that of T-SPOT.TB (84.00%, 100.00%), but there was no significant difference compared with CT (84.00%, 84.00%). ②The specificity of CT and T-SPOT.TB was lower than that of Mycobacterium tuberculosis culture (85.33%, 74.67%, 100.00%). There was no significant difference in the specificity of CT combined with T-SPOT.TB, GeneXpert MTB/RIF and Mycobacterium tuberculosis culture (97.33%, 100.00%, 100.00%). The specificity of CT combined with T-SPOT.TB was higher than that of each indicator alone (97.33%, 85.33%, 74.67%).③The accuracy of CT, T-SPOT.TB, CT combined with T-SPOT.TB and GeneXpert MTB/RIF was higher than that of Mycobacterium tuberculosis culture (84.67%, 87.33%, 90.67%, 81.33%, 54.67%, respectively). The accuracy of CT combined with T-SPOT.TB was higher than that of each indicator alone (90.67%, 84.67%, 87.33%). ④The AUC of CT, T-SPOT.TB, CT combined with T-SPOT.TB, GeneXpert MTB/RIF was higher than that of Mycobacterium tuberculosis culture (AUC=0.847, 0.873, 0.907, 0.813, 0.547). The AUC of CT combined with T-SPOT.TB was higher than that of each indicator alone (AUC=0.907, 0.847, 0.873). 
    Conclusion Spiral CT plain scan combined with T-SPOT TB and GeneXpert MTB/RIF can improve the clinical detection rate and pathogenic diagnostic efficacy of atypical renal tuberculosis. It is recommended to use the "Trinity Method" in clinical diagnosis of atypical renal tuberculosis. 

    The efficacy of different administration timing of somatostatin in the treatment of acute pancreatitis and analysis of plasma diamine oxidase activity
    XU Dong-ming
    2025, 46(2):  172-176.  doi:10.3969/j.issn.1007-3205.2025.02.008
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    Objective To analyze the efficacy of different administration timing of somatostatin in the treatment of acute pancreatitis and plasma diamine oxidase activity. 
    Methods A total of 96 patients with acute pancreatitis treated in Department of Emergency, Beijing Friendship Hospital, Capital Medical University were selected as observation subjects. Of them, 54 patients with mild and moderate disease were divided into group A (<24 h) and group B (≥24 h) according to whether the administration time of somatostatin was greater than 24 h, and 42 patients with severe disease were divided into group C (<24 h) and group D (≥24 h) according to whether the administration time of somatostatin was greater than 24 h. The impact of different administration timing on clinical efficacy was analyzed retrospectively. 
    Results The total clinical efficacy of group A was 96.30%, which was higher than that of group B, and the total clinical efficacy of group C was 85.71%, which was higher than that of group D (P<0.05). The remission time of abdominal pain and abdominal distension and average length of hospital stay in group A were shorter than those in group B (P<0.05). There was no significance in the incidence of complications between the two groups (P>0.05). The remission time of abdominal pain and abdominal distension and the average length of hospital stay in group C were shorter than those in group D (P<0.05). No significant difference was found in the incidence of complications between the two groups (P>0.05). After treatment, the plasma diamine oxidase activity was lower in group A than in group B, and lower in group C than in group D (P<0.05). There was no significant difference in the incidence of adverse reactions between group A and group B (P>0.05) as well as between group C and group D (P>0.05). 
    Conclusion Administration of somatostatin within 24 h after the onset of acute pancreatitis can effectively improve the clinical efficacy, shorten the duration of treatment, and promote the improvement of intestinal mucosal barrier function, with good safety. 

    Analysis of the impact of laparoscopic sleeve gastrectomy on long-term weight loss maintenance and glycemic stability in patients with type 2 diabetes mellitus and obesity
    HUANG Dan, LI Shu-fang, YU Wa-lin, ZHOU Gang
    2025, 46(2):  177-182.  doi:10.3969/j.issn.1007-3205.2025.02.009
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    Objective To investigate the effects of laparoscopic sleeve gastrectomy(LSG) on long-term weight loss maintenance and glycemic stability in patients with type 2 diabetes mellitus (T2DM) and obesity, and to explore the relationship between weight loss and glycemic stability. 
    Methods A total of 80 T2DM patients diagnosed with obesity in Jiangning Hospital of Nanjing City, Jiangsu Province, were selected as the research subjects, and underwent LSG. Body weight, body mass index (BMI), and waist circumference of the patients were compared before surgery and at various follow-up points within two years after the surgery. The glycemic profile [glycated hemoglobin (HbA1c), fasting plasma glucose (FPG), 2-hour postprandial blood glucose (2 hPBG)], HOMA model parameters [homeostatic model assessment of β-cell function (HOMA-β), homeostatic model assessment of insulin resistance (HOMA-IR)], insulin secretion index (△I10/△G10), and thyroid function indicators [thyroid-stimulating hormone (TSH), free triiodothyronine (FT3), free thyroxine (FT4)] before and after surgery were compared before and after surgery. Correlation analysis was performed based on the results between various indicators. 
    Results At various follow-up points within two years after intervention, body weight, waist circumference, BMI and blood glucose indexes were significantly lower than those before surgery, the postoperative HOMA-β and insulin secretion index were significantly higher than those before operation, the HOMA-IR was lower than that before surgery, and the TSH value of postoperative patients decreased significantly, while FT3 and FT4 showed a continuous upward trend, suggesting significant differences (all P<0.05). Correlation analysis showed that BMI was positively correlated with blood glucose index and thyroid hormone level, and negatively correlated with insulin secretion index (P<0.05). 
    Conclusion For patients with obesity and T2DM, blood glucose, insulin, and thyroid indicators all affect their weight and waist circumference. The application of LSG helps to maintain weight loss effects and improve glycemic stability, providing a more reliable treatment option for patients. 

    Correlation of preoperative sleep disturbance and nutritional status with postoperative delirium in elderly patients with colorectal cancer
    GUO Yan, TIAN En-qi, WANG Guo-ping
    2025, 46(2):  183-188.  doi:10.3969/j.issn.1007-3205.2025.02.010
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    Objective To assess the predictive value of preoperative sleep disturbance and nutritional status in postoperative delirium (POD) in elderly patients with colorectal cancer(CRC). 
    Methods A total of 276 elderly patients who underwent radical colon cancer surgery in our hospital from January 2019 to December 2022 were retrospectively included, and were divided into the POD group (n=66, 23.91%) and the control group (n=210, 76.09%)according to development of POD at 7 d postoperatively. The clinical data of CRC patients were collected through the inpatient electronic medical record system, and the risk factors for the development of POD in elderly CRC patients were analyzed using a multivariate logistic regression method. The clinical efficacy of preoperative sleep disturbance and nutritional status for predicting POD was evaluated using receiver operating characteristic (ROC) curves. 
    Results Univariate analysis showed that compared with the control group, the POD group had a significantly higher proportion of patients with age >70 years (68.18% vs. 48.57%), history of cerebrovascular disease (31.82% vs. 17.62%), TNM stage Ⅲ (43.94% vs. 29.52%), preoperative sleep disorders (59.09% vs. 34.76%), preoperative moderate to severe malnutrition (53.03% vs. 29.52%), and significantly prolonged duration of anaesthesia [(4.29±1.13) h vs. (3.92±0.86) h], with a significant difference (P<0.05). Multivariate Logistic regression analysis showed that age >70 years (OR=1.390, 95%CI: 1.062-1.819), preoperative sleep disturbance (OR=1.752, 95%CI: 1.302-2.359), and preoperative moderate to severe malnutrition (OR=1.891, 95%CI: 1.256-2.846) were independent risk factors for elderly CRC patients combined with POD. The ROC curve showed that the area under the ROC curve (AUC) of preoperative sleep disturbance combined with moderate to severe malnutrition in predicting the development of POD in elderly CRC patients was 0.735, with sensitivity of 89.39% and specificity of 57.62% . 
    Conclusion Preoperative sleep disturbance and malnutrition are independent risk factors for the development of POD in elderly CRC patients, and the combined detection can predict the risk of POD, to assist in the optimization of perioperative management and reduce the incidence of POD. 

    Application effect of bronchial blocker in thoracoscopic surgery with one-lung ventilation in children aged 2-9 years and its impact on intraoperative lung collapse score
    WU Na, CHEN Chao, ZHANG Yan-yan
    2025, 46(2):  189-194.  doi:10.3969/j.issn.1007-3205.2025.02.011
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    Objective To explore the application effect of bronchial blockers in thoracoscopic surgery with one-lung ventilation (OLV) in children aged 2-9 years and their impact on the intraoperative lung collapse score (LCS). 
    Methods Eighty children aged 2-9 years admitted to the Children's Hospital of Xuzhou City were randomly divided into the tracheal group (n=40) and the bronchial group (n=40) using envelope drawing. The tracheal group underwent thoracoscopic surgery with OLV via a tracheal catheter, while the bronchial group underwent thoracoscopic surgery with OLV via a bronchial blocker. Perioperative indicators were compared between the two groups. Mean arterial pressure (MAP), heart rate (HR), and blood gas analysis before ventilation (T1), during ventilation (T2), at 20 min after initiation of ventilation (T3) and at 40 min after initiation of ventilation (T4), as well as quality of recovery 15 (QoR-15) scores and LCS during surgery, and complications, were compared. 
    Results The duration of surgery (208.37±24.28) min, duration of intubation (2.69±0.23) min, and catheter displacement rate (2.5%) in the bronchial group were lower than those in the tracheal group [(21.87±28.34) min, (3.74±0.28) min, 17.5%], and the first attempt intubation success rate (100.0%) was higher than that in the tracheal group (90.0%) (χ2/t=2.779, 22.258, 5.000, 4.211, P<0.05). There was no significant difference in interactions between groups, and time points between groups in terms of MAP (P>0.05), but the difference was significant with respect to time points (F time points=5.236, P<0.05). For HR, no significant differences of interaction were found between groups, time points, and time points between groups (P>0.05). The partial pressure of oxygen (PaO2) in arterial blood in the bronchial group gradually decreased with time, while in the tracheal group it initially decreased and then increased. Significant differences of interaction were found between groups, time points, and time points between groups (F between groups = 118.084, F time points = 175.854, F time points between groups = 9.310, all P<0.05). The partial pressure of carbon dioxide (PaCO2) in arterial blood and oxygen saturation (SpO2) in both groups gradually decreased over time. The difference of interaction between groups, time points and time points between groups was statistically significant (F between groups = 16.742, 151.743, F time points = 33.740, 84.641, F time points between groups = 8.357, 45.565, all P<0.05). The intraoperative LCS score (6.70±0.56) and QoR-15 score (102.33±12.20) at 1 day after surgery in the bronchial group were higher than those in the tracheal group (5.35±0.47, 89.60±10.10) (t=14.184, 6.17, P<0.05). There was no significant difference in the incidence of complications between the tracheal and bronchial groups (P>0.05). 
    Conclusion The use of bronchial blockers in thoracoscopic surgery with OLV for children aged 2-9 is effective and significantly influences the intraoperative LCS. 

    Predictive value of preoperative frailty for chronic post-surgical pain in elderly patients in department of thoracic surgery
    MENG Zhen-ang, CHEN Li-li, FAN Jin-yan, ZHANG Chuan-wu, WANG Guang-lei
    2025, 46(2):  195-201.  doi:10.3969/j.issn.1007-3205.2025.02.012
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    Objective To evaluate the predictive value of preoperative frailty for chronic post-sugrical pain (CPSP) in elderly patients in department of thoracic surgery. 
    Methods In total, 318 patients underwent elective thoracic surgery under general anesthesia, who were aged 65 years or older, with American society of anesthesiologists (ASA) Ⅱ-Ⅳ and regardless of gender. Frailty was assessed using FRAIL Scale at 1 d before surgery. At 24 h and 48 h after surgery, the numerical rating scale (NRS) was used to record patient pain scores at rest and on movement, and telephone follow-up was performed at 3 months after surgery, to record the patient's pain scores. NRS score>0 represented the occurrence of CPSP. Patients were divided into CPSP and non-CPSP groups based on whether CPSP occurred at 3 months after surgery. Multivariate Logistics regression analysis was used to screen risk factors for CPSP in elderly patients undergoing thoracic surgery and receiver operating characteristic (ROC) curve was used to analyze the predictive value of preoperative frailty on CPSP. 
    Results Of 273 patients enrolled, 64 patients had preoperative frailty, with an incidence of 23.4%, and 75 patients developed CPSP, with an incidence of 27.5%. Univariate Logistic regression results showed that there were significant differences in both groups with respect to gender, age, ASA grade, diabetes, duration of thoracic drainage, mean NRS score at rest at 48 h after surgery, number of incisions and frailty grade. The results of multivariate Logistics regression analysis showed that female (OR 2.545, 95%CI: 1.365-4.746, P=0.003), preoperative frailty (OR 3.272, 95%CI: 2.146-4.853, P<0.001), duration of thoracic drainage (OR 1.250, 95%CI: 1.079-1.449, P= 0.003) and the mean NRS score at rest at 48 h after surgery (OR value 1.457, 95%CI: 1.081-1.963, P=0.014) were closely related with occurrence of CPSP in elderly patients undergoing thoracic surgery. The area under the ROC curve (AUC) of preoperative frailty in predicting CPSP was 0.705, with 95%CI: 0.634-0.776, sensitivity of 0.493, specificity of 0.843. 
    Conclusion Female gender, preoperative frailty, prolonged time of thoracic drainage and elevated mean NRS score at rest at 48 h after surgery are independent risk factors for CPSP in elderly patients undergoing thoracic surgery. Routine preoperative frailty screening can predict the occurrence of CPSP to some extent. 

    Mechanism of MiR-1-3p in apoptosis of retinal ganglion cells in glaucoma rats via VEGF/Notch signaling pathway
    GU Dan, LUO Na
    2025, 46(2):  202-207.  doi:10.3969/j.issn.1007-3205.2025.02.013
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    Objective To investigate the effect of miR-1-3p on apoptosis of retinal ganglion cells in glaucoma rats and its regulatory mechanism. 
    Methods Thirty-six one-month-old SPF SD rats were given adaptive feeding for one week, and then divided into normal group (n=12), model group (n=12) and miR-1-3p antagonistic group (n=12) by random number table method. The normal group did not receive any treatment. The rats in the model group were used to construct glaucoma model, and 0.2 mL sterile saline was injected intraperitoneally every day for 7 days. The rats in the miR-1-3p antagonist group were used to construct glaucoma model, and 0.2 mL of 3 μmol/L miR-1-3p antagonist was injected intraperitoneally every day after surgery for continuous intervention for 7 days. Intraocular pressure (IOP) was measured at the end of glaucoma modeling and the last intervention. The levels of Notch1 and Notch2 in the retinal tissues of each group were measured by Western blotting, and vascular endothelial growth factor (VEGF) content was detected by enzyme-linked immunosorbent assay (ELISA). The mRNA expression levels of VEGF, Notch1 and Notch2 in retinal tissues of rats were determined by qRT-PCR, and apoptosis was determined by TdT-mediated biotinylated-dUTP nick end labeling (TUNEL) assay. 
    Results At the end of modeling, the IOP of model group and miR-1-3p antagonist group was significantly higher than that of normal group (P<0.05). At the end of the last intervention, the IOP of miR-1-3p antagonist group was significantly lower than that of model group (P<0.05). VEGF levels and Notch1 and Notch2 protein expression levels in retinal tissues of model group were significantly higher than those of normal group (P<0.05). Compared with the model group, VEGF levels and Notch1 and Notch2 protein expression levels in retinal tissues of miR-1-3p antagonistic group were significantly decreased (P<0.05). The mRNA expression levels of VEGF, Notch1, Notch2 and apoptosis levels in retinal tissues in model group were significantly higher than those in normal group (P<0.05). Compared with the model group, mRNA expression levels of VEGF, Notch1, Notch2 and apoptosis levels in retinal tissues of miR-1-3p antagonist group were significantly decreased (P<0.05). 
    Conclusion MiR-1-3p promotes apoptosis of retinal ganglion cells in glaucoma rats by promoting VEGF/Notch signaling pathway, which is a potential molecular target for the treatment of glaucoma. 

    The application value of CGF fibrin solution combined with Bio-Oss bone powder in the repair of jawbone defects
    LU Shen-chen, LIN Hou-xue, ZHANG Ding-cheng
    2025, 46(2):  208-213,封三.  doi:10.3969/j.issn.1007-3205.2025.02.014
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    Objective To explore the application value of concentrated growth factor (CGF) fibrin solution combined with Bio-Oss bone powder in the repair of jawbone defects. 
    Methods Eighty patients with jawbone defects after jaw cyst surgery in the 17th Metallurgical Hospital were selected and divided into an observation group (n=40) and a control group (n=40) using a random number table method. The control group was treated with Bio-Oss bone powder, while the observation group was supplemented with CGF fibrin solution on the basis of the control group. The healing of incisions in the two groups was compared, the computed tomography (CT) values of the bone defect filling area were measured, and the bone resorption, bone density, jawbone height and width, and incidence of complications between the two groups were detected. 
    Results The observation group showed better wound healing than the control group (P<0.05). The CT values of the bone defect filling areas in both groups showed a trend of first increasing and then decreasing, reaching a peak at one month after surgery. The CT values of the bone defect filling areas in the observation group were higher than those in the control group, and there were significant differences in the interaction between groups, time points, and time points between groups (P<0.05). At 6 months after surgery, the bone resorption and bone density of both groups were higher than those at 3 months after surgery, and the height of the maxilla as well as the height and width of the mandible was higher than that at 3 months after surgery (P<0.05). At 3 months and 6 months after surgery, the bone resorption and bone density of the observation group were lower than those of the control group, while the height of the maxilla as well as the height and width of the mandible was higher than that of the control group (P<0.05). The total incidence of complications in the observation group was lower than that in the control group (P<0.05). 
    Conclusion The use of CGF fibrin solution combined with Bio-Oss bone powder for the treatment of patients with jawbone defects after after jawcyst surgery can not only promote bone repair, bone healing, and jawbone shape recovery, but also reduce bone resorption, and improve bone density, with good safety. 

    The diagnostic value of multimodal MRI combined with tumor markers CA19-9, CA72-4, CEA, and AFP in preoperative T staging and EMVI of rectal cancer
    WANG Wan-ting, DAN Ba-yu-zhen
    2025, 46(2):  214-219.  doi:10.3969/j.issn.1007-3205.2025.02.015
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    Objective To explore the diagnostic value of multimodal magnetic resonance imaging (MRI) combined with tumor markers [carbohydrate Antigen 19-9 (CA19-9), cancer antigen 72-4 (CA72-4), carcinoembryonic antigen (CEA), alpha-fetoprotein (AFP)] in the preoperative T staging and extramural venous invasion (EMVI) of rectal cancer. 
    Methods A retrospective analysis was conducted on 136 patients with rectal cancer treated at the Third People′s Hospital of Chengdu City. Using postoperative pathological staging as the gold standard, all patients underwent multimodal MRI and levels of CA19-9, CA72-4, CEA, and AFP were detected. Based on results of pathological diagnosis, patients were divided into positive EMVI group (n=58) and negative EMVI group (n=78). MRI results and levels of CA19-9, CA72-4, CEA, and AFP in the two groups were compared, and the diagnostic efficacy of multimodal MRI combined with these tumor markers in preoperative T staging and EMVI was analyzed using receiver operating characteristic (ROC) curves. 
    Results Preoperative T staging showed 12 patients in T1, 22 in T2, 68 in T3, and 34 in T4. By multimodal MRI, 8 patients were diagnosed as T1, 16 as T2, 59 as T3, and 29 as T4. The levels of CA19-9 (43.14±4.28) kU/L, CA72-4 (12.88±2.69) kU/L, CEA (23.11±2.89) μg/L, and AFP (21.25±2.64) μg/L in T3-T4 were higher than those in T1-T2 (10.33±2.03) kU/L, (6.12±1.47) kU/L, (13.15±3.24) μg/L, (16.25±3.27) μg/L (P<0.05). Positive EMVI group had higher Ktrans, Kep, and lower ADC compared with the negative EMVI group; The levels of CA19-9 (55.87±5.63) kU/L, CA72-4 (14.92±3.12) kU/L, and CEA (14.89±2.14) μg/L were higher than those in the negative EMVI group (9.42±2.01) kU/L, (7.21±1.54) kU/L, (7.84±1.56) μg/L (P<0.05). ROC analysis showed that multimodal MRI combined with tumor markers in EMVI diagnosis had a sensitivity of 93.1% and a specificity of 97.4%, with an area under the ROC curve (AUC) of 0.975 (95%CI: 0.946-1.000), which was higher than single-item detection. 
    Conclusion Multimodal MRI combined with CA19-9, CA72-4, CEA, and AFP levels demonstrates high sensitivity and specificity in the diagnosis of EMVI of rectal cancer, significantly outperforming detection by each indicator alone. It provides a more accurate diagnostic basis for the preoperative assessment of rectal cancer.