miR146干预PI3K/Akt信号通路对类风湿关节炎滑膜成纤维细胞的影响

doi:10.3969/j.issn.10073205.2019.06.008

摘要/Abstract

摘要： ［摘要］〓
〖HTH〗目的〖HTSS〗〖KG*2〗探讨微小RNA(microRNA,miR)146通过PI3K/Akt信号通路对类风湿关节炎(rheumatoid arthritis,RA)滑膜成纤维细胞(fibroblastlike synoviocytes,FLS)的影响。
〖HTH〗方法〖HTSS〗〖KG*2〗将RAFLS细胞分为对照组、脂多糖(lipopolysaccharide，LPS)组和LPS+miR146组。LPS+miR146组转染miR146后，LPS组和LPS+miR146组分别与LPS共孵育24 h，噻唑蓝法检测细胞增殖能力，流式细胞仪检测细胞凋亡情况，酶联免疫吸附测定法检测上清液白细胞介素1β(interleukin1β，IL1β)、白细胞介素6(interleukin6，IL6)和肿瘤坏死因子α(tumor necrosis factorα，TNFα)水平，实时定量聚合酶链反应法检测miR146、PI3K及Akt mRNA表达，蛋白免疫印迹法检测PI3K、Akt和磷酸化（p）Akt表达。
〖HTH〗结果〖HTSS〗〖KG*2〗与LPS组比较，LPS+miR146组miR146表达明显增加（P＜005），RAFLS细胞增殖能力明显下降（P＜005），且细胞凋亡明显增加（P＜005），上清液IL1β、IL6和TNFα含量明显减少（P＜005），PI3K mRNA和蛋白表达明显下调（P＜005），Akt mRNA及蛋白表达无明显变化（P＞005），而pAkt蛋白表达明显下降（P＜005）。
〖HTH〗结论〖HTSS〗〖KG*2〗miR146可能通过干预PI3K/Akt信号通路调控RAFLS细胞增殖、凋亡及炎症因子产生。

关键词: 关节炎, 类风湿, 微小RNA146, 磷酸肌醇3激酶类

Abstract: ［Abstract］ Objective〖HTSS〗〓To investigate the effect of microRNA(miR146) on rheumatoid arthritis(RA) fibroblastlike synoviocytes(FLS) through PI3K/Akt signaling pathway.
〖HTH〗〖WTHZ〗Methods〖HTSS〗〓RAFLS cells were divided into the control group, lipopolysaccharide(LPS) group and LPS+miR146 group. After transfection of miR146 in the LPS+miR146 group, the LPS group and the LPS+miR146 group were incubated with LPS for 24 h, respectively. The cell proliferation was determined by methylthiazolyldiphenyltetrazolium. The Flow cytometry was used to detect cell apoptosis. The levels of interleukin1β(IL1β), interleukin6(IL6) and tumor necrosis factorα(TNFα) in supernatants were determined by enzymelinked immunosorbent assay. The realtime quantitative polymerase chain reaction was used to detect the expression of miR146, PI3K and Akt mRNA. Western blotting was used to detect the expression of PI3K, Akt and phosphorylated(p)Akt.
〖HTH〗〖WTHZ〗Results〖HTSS〗〓Compared with LPS group, the expression of miR146 in LPS+miR146 group was significantly increased(P＜005), the proliferation of RAFLS cells was significantly decreased(P＜005), and the apoptosis was significantly increased(P＜005). Compared with LPS group, the contents of IL1β, IL6 and TNFα in the supernatant in LPS+miR146 group were significantly decreased(P＜005), the expression of PI3K mRNA and protein in LPS+miR146 group was downregulated(P＜005), and the expression of Akt mRNA and protein in LPS+miR146 group was not significantly changed(P＞005), and the expression of pAkt protein in LPS+miR146 group was significantly decreased(P＜005).
〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓miR146 may regulate the proliferation, apoptosis and inflammatory factors of RAFLS cells by interfering with PI3K/Akt signaling pathway.

Key words: arthritis, rheumatoid, microRNA146, phosphoinositide 3kinase

黄〓勤，毛慧慧. miR146干预PI3K/Akt信号通路对类风湿关节炎滑膜成纤维细胞的影响[J]. 河北医科大学学报, doi: 10.3969/j.issn.10073205.2019.06.008.

HUANG Qin, MAO Huihui. Effect of miR146 on rheumatoid arthritis synovial fibroblasts through PI3K/Akt signaling pathway[J]. Journal of Hebei Medical University, doi: 10.3969/j.issn.10073205.2019.06.008.

[1]	杨学钰, 郭文帆, 郑雪君, 卢吉高, 杨勇, 李会东. HIF-1α通过炎性小体ASC结构域调控膝骨关节炎的分子机制研究 [J]. 河北医科大学学报, 2023, 44(6): 692-696,706.
[2]	孙超, 韩城旭, 齐晅, 田玉, 高丽霞, 郭惠芳. 类风湿关节炎膝股骨髁软骨厚度与临床特征的相关性 [J]. 河北医科大学学报, 2023, 44(11): 1289-1294.
[3]	胡欣怡（综述）, 荣晓凤（审校）. 中轴型脊柱关节炎的病理变化与生物标志物 [J]. 河北医科大学学报, 2023, 44(11): 1360-1364.
[4]	王婵, 王俊华, 左薇, 许明军, 张刚. 肠道微生态调节剂影响类风湿关节炎患者肠道微生物组和免疫微环境的临床研究 [J]. 河北医科大学学报, 2023, 44(1): 25-29,61.
[5]	严斌, 刘刚, 张林, 刘青柏. 自体PRP注射联合依托考昔治疗膝关节骨性关节炎效果及对细胞因子的影响 [J]. 河北医科大学学报, 2023, 44(1): 57-61.
[6]	冯彦华, 任强, 甄磊, 郝建宗, 张放, 田苡任. 血清学指标水平变化对儿童急性骨关节感染继发脓毒症的早期预测价值分析[J]. 河北医科大学学报, 2022, 43(8): 940-944.
[7]	辜伟. 关节镜下清理术联合富血小板血浆治疗中重度膝骨性关节炎的效果研究[J]. 河北医科大学学报, 2022, 43(4): 402-406，411.
[8]	袁健生, 周念, 蔡甜, 杜燕娇, 贾岩, 蒋子裕. 血清淀粉样蛋白A、血管内皮生长因子与类风湿关节炎相关性研究[J]. 河北医科大学学报, 2022, 43(2): 213-217.
[9]	胡恒, 彭伟, 张忠. 双醋瑞因胶囊结合艾瑞昔布片对膝骨关节炎患者的治疗效果及对COX-2、Wnt-3α、骨代谢水平的影响[J]. 河北医科大学学报, 2022, 43(2): 223-228.
[10]	童俊. 基于MRI影像特征、定量参数构建类风湿性关节炎治疗效果的评估模型 [J]. 河北医科大学学报, 2022, 43(10): 1197-1202.
[11]	刘俊鹏, 韩文彪, 刘小会, 刘广召, 任玉娥. 膝关节腔注射糖皮质激素对血糖、血脂及HPA轴的影响[J]. 河北医科大学学报, 2021, 42(5): 541-546.
[12]	王欣彤, 周朝波, 杨丽晖, 闫立强, 王锴, 王茜. 个性化神经肌训练对膝关节骨关节炎患者膝关节功能、疼痛、僵硬症状、生活质量的影响研究[J]. 河北医科大学学报, 2021, 42(4): 395-400.
[13]	李萍, 柴恺. CR假体与PS假体对全膝关节置换术疗效的影响[J]. 河北医科大学学报, 2021, 42(1): 42-76.
[14]	陈立涛, 张磊, 周谨, 赵保礼, 赵丽华, 杜志兴. 臭氧治疗膝关节创伤性关节炎前瞻性研究[J]. 河北医科大学学报, 2020, 41(12): 1469-1472.
[15]	王弘德，李升，陈伟，王天瑞，张英泽，王娟*. 《骨关节炎诊疗指南( 2018 年版)》膝关节骨关节炎部分的更新与解读[J]. 河北医科大学学报, 2019, 40(9): 993-995,1000.

miR146干预PI3K/Akt信号通路对类风湿关节炎滑膜成纤维细胞的影响

Effect of miR146 on rheumatoid arthritis synovial fibroblasts through PI3K/Akt signaling pathway

HTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价