湿行瘀清热方治疗金黄地鼠颊黏膜癌前病变后HSP90α、Ki67的表达及意义

doi:10.3969/j.issn.10073205.2017.10.015

河北医科大学学报

湿行瘀清热方治疗金黄地鼠颊黏膜癌前病变后HSP90α、Ki67的表达及意义

1.河北省胸科医院口腔科，河北石家庄 050041；2.河北医科大学第四医院口腔科，河北石家庄 050011

出版日期:2017-10-25 发布日期:2017-10-18
作者简介:张鹏飞（1981-），男，山西运城人，河北省胸科医院主治医师，医学硕士，从事口腔修复研究。
基金资助:
河北省中医药管理局科研计划（2016175）

The expressions and significance of HSP90α and Ki67 in golden hamster buccal mucosa precancerous lesions after Huashi Xingyu Qingre decoction treatment#br#

1.Department of Stomatology, the Chest Hospital of Hebei Province, Shijiazhuang 050041, China;
2.Department of Stomatology, the Second Hospital of Hebei Medical University, Shijiazhuang 050011, China

Online:2017-10-25 Published:2017-10-18

摘要/Abstract

摘要： ［摘要］〓
〖HTH〗目的〖HTSS〗〖KG*2〗探讨金黄地鼠颊黏膜癌前病变经化湿行瘀清热方治疗前后热休克蛋白（heat shock protein 90α,HSP90α）、Ki67的表达及临床意义。
〖HTH〗方法〖HTSS〗〖KG*2〗60只金黄地鼠用二羟甲基丁酸（dimethylolbutanoie acid，DMBA）丙酮溶液涂抹6周全部制作成金黄地鼠颊黏膜癌前病变的动物模型，随机分成实验组和对照组。实验组30只应用化湿行瘀清热方行灌胃疗法8周，对照组30只应用生理盐水灌胃8周，采用免疫组织化学SP法观察2组口腔黏膜的形态变化，应用染色强度评价HSP90α、Ki67的表达水平。
〖HTH〗结果〖HTSS〗〖KG*2〗灌胃后实验组Ki67阳性表达率为23.3%（7/30），对照组Ki67阳性表达率为80.0%（24/30），2组差异有统计学意义(P＜001）；灌胃后实验组HSP90α阳性表达率为20.0%（6/30），对照组HSP90α阳性表达率为76.7%（23/30），2组差异有统计学意义(P＜001）。
〖HTH〗结论〖HTSS〗〖KG*2〗化湿行瘀清热方对DMBA诱发的金黄地鼠口腔黏膜癌前病变有一定的逆转作用。

关键词: 癌前状态, 热休克蛋白质类, Ki67, 化湿行瘀清热方

Abstract: ［Abstract］ Objective〖HTSS〗〓To investigate the expressions and significance of heat shock protein 90α, HSP90α）and Ki67 in hamster buccal mucosa precancerous lesions after Huashi Xingyu Qingre decoction treatment.
〖HTH〗〖WTHZ〗Methods〖HTSS〗〓Sixty golden hamster models of buccal mucosa precancerous lesions were established after the contact of dimethylolbutanoie acid(DMBA) for six weeks. They were randomly divided into experimental and control groups. Thirty rats of experimental group were received gavage therapy of Huashi Xingyu Qingre decoction for 8 weeks, while, the other thirty rats of control group were received normal saline treatment for 8 weeks. The expression levels of HSP90α、Ki67 were assessed by dyeing intensity of streptavidinperosidase(SP).
〖HTH〗〖WTHZ〗Results〖HTSS〗〓The positive expression rate of Ki67 after gavage was 23.3%(7/30) in the experimental group and 80.0%(24/30) in the control group(P＜0.01). The positive expression rate of HSP90α after gavage was 20.0%(6/30) in the experimental group and 76.7%(23/30) in the control group(P＜0.01).
〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓Huashi Xingyu Qingre decoction is conspicuously effective in the reversal of DMBAinduced precancerous lesions in the hamster buccal mucosa.

Key words: precancerous lesions, heatshock proteins, Ki67, Huashi Xingyu Qingre decoction

张鹏飞1，刘〓健2，刘铁军2，许彦枝2*. 湿行瘀清热方治疗金黄地鼠颊黏膜癌前病变后HSP90α、Ki67的表达及意义[J]. 河北医科大学学报, doi: 10.3969/j.issn.10073205.2017.10.015.

ZHANG Pengfei1, LIU Jian2, LIU Tiejun2, XU Yanzhi2*. The expressions and significance of HSP90α and Ki67 in golden hamster buccal mucosa precancerous lesions after Huashi Xingyu Qingre decoction treatment#br#[J]. Journal of Hebei Medical University, doi: 10.3969/j.issn.10073205.2017.10.015.

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湿行瘀清热方治疗金黄地鼠颊黏膜癌前病变后HSP90α、Ki67的表达及意义

The expressions and significance of HSP90α and Ki67 in golden hamster buccal mucosa precancerous lesions after Huashi Xingyu Qingre decoction treatment#br#

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