关键词: 骨肉瘤, 细胞增殖, 微RNAs

Abstract: ［Abstract］ Objective〖HTSS〗〓To find out the function and mechanism of microRNA32（miR32） in osteosarcoma cell proliferation.
〖HTH〗〖WTHZ〗Methods〖HTSS〗〓Reverse transcriptionpolymerase chain reaction（RTPCR） was used to detect the expression of miR32 in osteosarcoma tissues, normal tissues and different cell lines. The effect of inhibiting miR32 expression on the proliferation rate of human osteosarcoma MG63 cell line was detected by CCK8. Luciferase assay showed that miR32 could bind specifically to the 3′UTR of phosphatase and tensin homolog deleted on chromosome ten（PTEN） gene. At the same time, RTPCR and western blotting were used to verify the relationship between miR32 and PTEN from RNA and protein level respectively.
〖HTH〗〖WTHZ〗Results〖HTSS〗〓miR32 in 〖JP2〗osteosarcoma tissues and cells activate PI3K/Akt signaling pathway after binding to PTEN′s〖JP〗 3′UTR, which further influences the proliferation of MG63 cells. 
〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓miR32 regulates the proliferation of osteosarcoma cells through PI3K/Akt signaling pathway.

Key words: osteosarcoma； cell proliferation, microRNAs