河北医科大学学报

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降钙素基因相关肽参与调节Th17和Treg细胞分化

  

  1. 1.河北医科大学公共卫生学院流行病与卫生统计学教研室，河北 石家庄 050017；2.河北医科大学第一医院超声科，
    河北 石家庄 050031；3.河北医科大学基础医学院免疫学教研室，河北 石家庄 050017；
    4.辽宁省辽阳市中心医院胸外科，辽宁 辽阳 111000
  • 出版日期:2017-03-25 发布日期:2017-03-29
  • 作者简介:刘文宣(1983-)，女，河北栾城人，河北医科大学公共卫生学院副教授，医学博士,从事类风湿性关节炎免疫机制研究。
  • 基金资助:
    河北省高等学校科学研究计划（QN2015006）；河北省医学科学研究重点课题（20150626，20150631）

Calcitonin gene related peptide participate in the regulation of Th17 and Treg differentiation

  1. 1.Department of Epidemiology and Statistics, School of Public Health， Hebei Medical University,
    Shijiazhuang 050017, China；2.Department of Ulstrasound, the First Hospital of Hebei Medical
    University, Shijiazhuang 050031, China；3.Department of Immunology, Institute of Basic
    Medicine, Hebei Medical University, Shijiazhuang 050017, China; 4.Department of
    Thoracic Surgery, the Central Hospital of Liaoyang City, Liaoyang 111000, China
  • Online:2017-03-25 Published:2017-03-29

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摘要： ［摘要］〓
〖HTH〗目的〖HTSS〗〖KG*2〗研究降钙素基因相关肽（calcitonin gene related peptide, CGRP）对人外周血中Th17（IL17 producing T cell）和Treg（regulatory T cell）细胞分化的调节作用。
〖HTH〗方法〖HTSS〗〖KG*2〗采用免疫磁珠法（magnetic activated cell sorting，MACS）分离人外周血中CD4+ CD45RA+ T细胞，即初始CD4+T细胞，在一定条件下分别体外诱导其向Th17细胞和Treg细胞分化，之后用流式细胞术测定Th17和Treg细胞所占比例；用实时定量RTPCR方法检测Th17和Treg细胞分化的特异性转录因子维甲酸相关核孤儿受体γt（orphan nuclear receptor γt，RORγt）和转录因子FoxP3 （foxhead box protein 3）mRNA的表达水平；用酶联免疫吸附测定法（enzymelinked immunosorbent assay，ELISA）检测Th17细胞上清中IL17A的含量，进而从多个方面探讨CGRP对Th17和Treg细胞分化的影响。
〖HTH〗结果〖HTSS〗〖KG*2〗CGRP可剂量依赖性增加Th17细胞所占的比例和RORγt mRNA的表达水平（P＜005）；另外，CGRP也促进了IL17A的分泌（P＜005）。同时，CGRP能剂量依赖性抑制Treg细胞所占的比例和FoxP3 mRNA的表达（P＜005）。
〖HTH〗结论〖HTSS〗〖KG*2〗CGRP可促进人外周血中初始CD4+T细胞向Th17细胞分化，而抑制其向Treg细胞分化。

关键词: 降钙素, Th17细胞, T淋巴细胞, 调节性

Abstract: ［Abstract］ Objective〖HTSS〗〓To dissect the role of calcitonin gene related peptide(CGRP) on IL17 producing T cell(Th17) and regulatory T cell(Treg) differentiation.
〖HTH〗〖WTHZ〗Methods〖HTSS〗〓Naive CD4+T cells were isolated from human peripheral blood by magnetic activated cell sorting(MACS) selection, and cultured under Th17polarizing and Tregpolarizing condition, The proportion of Th17 cells and Treg cells were detected by flow cytometry, the relative expression levels of retionidrelated orphan nuclear receptor γt(RORγt) mRNA and foxhead box protein 3(FoxP3) mRNA were measured by realtime RTPCR. In addition, we detected the production of IL17A by enzymelinked immunosorbent assay, we explored the effects of CGRP on the differentiation of Th17 and Treg cells form Several aspects.
〖HTH〗〖WTHZ〗Results〖HTSS〗〓CGRP increased the proportion of Th17 cells and the expression of RORγt mRNA(P＜005). Additionally, CGRP also increased the production of IL17A(P＜005), these effects of CGRP were in dosedependent manner. Meanwhile, CGRP inhibited the percentage of Treg cells and the expression of FoxP3 mRNA also in dosedependent way(P＜005), peaking at 10-6 M.
〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓These results demonstrate that CGRP facilitates naive CD4+T cells from human peripheral blood differentiate to Th17 cells, but suppresses them differentiate to Treg cells.

Key words: calcitonin, Th17 cells ；Tlymphocytes, regulatory

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