被动颗粒凝集法检测MP抗体效价和胶体金法联合检测MPIgM、MPIgG抗体在儿童支原体肺炎中的应用价值

doi:10.3969/j.issn.10073205.2018.09.015

河北医科大学学报

被动颗粒凝集法检测MP抗体效价和胶体金法联合检测MPIgM、MPIgG抗体在儿童支原体肺炎中的应用价值

1.河北省儿童医院检验科，河北石家庄 050031；2.河北省儿童医院儿研所，河北石家庄 050031

出版日期:2018-09-25 发布日期:2018-08-28
作者简介:张文超（1977-），男，河北保定人，河北省儿童医院主管检验师，医学学士，从事临床检验学研究。

The clinical value of pellet agglutination assay for MP antibody titer and colloidal gold method for simultaneously detection of MPIgM and MPIgG in children with mycoplasma pneumonia#br#

1. Department of Clinical Laboratory，Children′s Hospital of Hebei Province，Shijiazhuang 050031， China;
2.Pediatric Research Institute， Children′s Hospital of Hebei Province， Shijiazhuang 050031， China

Online:2018-09-25 Published:2018-08-28

摘要/Abstract

摘要： ［摘要］〓
〖HTH〗目的〖HTSS〗〖KG*2〗探讨MPIgM、MPIgG二联检测试剂（胶体金法）联合检测MPIgM、MPIgG抗体对儿童肺炎支原体（mycoplasma pneumonia, MP）感染的临床诊断价值。
〖HTH〗方法〖HTSS〗〖KG*2〗选择疑似MP感染者344例,使用胶体金法联合检测MPIgM、MPIgG抗体，同时使用被动颗粒凝集法检测患者的MP抗体效价，比较2种方法在儿童MP肺炎中的诊断价值。
〖HTH〗结果〖HTSS〗〖KG*2〗以滴度1〖DK〗∶160为抗体阳性，MP阴性233例(67.7%)，MP阳性111(32.3%)例。MPIgM抗体检测试剂盒和MPIgG抗体检测试剂盒检测结果与MPIgM、MPIgG二联检测试剂盒检测结果的吻合度较高，一致性较好。以临床确诊结果为金标准，MP抗体效价检测以滴度1〖DK〗∶40为阳性界值时和以滴度1〖DK〗∶160为阳性界值时，敏感度和阴性预测值均为100.0%，以滴度1〖DK〗∶160为阳性界值时特异度、准确度和阳性预测值明显高于以滴度1〖DK〗∶40为阳性界值时。MPIgM和MPIgG的敏感度、特异度、阳性预测值、阴性预测值及准确度差异不大，与以滴度1〖DK〗∶160为阳性界值的MP抗体效价检测方法各项指标基本一致。
〖HTH〗结论〖HTSS〗〖KG*2〗临床疑似MP感染者采用MPIgM、MPIgG二联检测试剂（胶体金法）快速检测MPIgM和MPIgG，可提高疾病诊断率，缩短病程，避免交叉感染，减轻患者及社会负担。

关键词: 肺炎, 支原体, 免疫球蛋白M, 免疫球蛋白G

Abstract: ［Abstract］〓Objective〖HTSS〗〓To explore the clinical diagnosis value of colloidal gold method for simultaneously detection of MPIgM and MPIgG in children with mycoplasma pneumoniae(MP) infection.
〖HTH〗〖WTHZ〗Methods〖HTSS〗〓The 344 suspected patients with MP infection were studied. MP antibody titer was detected by pellet agglutination assay, and MPIgM and MPIgG were simultaneously detected by colloidal gold method. Consistency of the results tested by the two methods was compared.
〖HTH〗〖WTHZ〗Results〖HTSS〗〓When the antibody titers of 1〖DK〗∶160 were considered as positive by pellet agglutination assay, 233(67.7%) cases were negative for MP and 111(32.3%) cases were positive for MP. The results tested by diagnostic kit for IgM antibody to MP and diagnostic kit for IgG antibody to MP were roughly consistent with diagnostic kit for IgM and IgG antibody to MP. Using the results of clinical diagnosis as the gold standard, the sensitivity and negative predictive value of pellet agglutination assay were 100.0% when the critical value at the antibody titers of 1〖DK〗∶40, which was accordant with those of pellet agglutination assay when the critical value at the antibody titers of 1〖DK〗∶160. However, the specific, positive predictive value and accuracy of pellet agglutination assay when the critical value at the antibody titers of 1〖DK〗∶40 were significantly lower than those of pellet agglutination assay when the critical value at the antibody titers of 1〖DK〗∶160. The sensitivity, specific, positive predictive value, negative predictive value and accuracy difference between MPIgM, MPIgG and pellet agglutination assay when the critical value at the antibody titers of 1〖DK〗∶160 were basically identical.
〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓Colloidal gold method can simultaneously detect MPIgM and MPIgG of clinical suspected with MP infection, thus improving disease diagnosis rate, shortening the course of the disease, avoiding crossing infection and reducing the financial burden on patients and society.

Key words: pneumonia, mycoplasma, immunoglobulin M, immunoglobulin G

张文超1，赵梦川2，冯志山1，李〓梅1，郭映辉1，李贵霞1*. 被动颗粒凝集法检测MP抗体效价和胶体金法联合检测MPIgM、MPIgG抗体在儿童支原体肺炎中的应用价值[J]. 河北医科大学学报, doi: 10.3969/j.issn.10073205.2018.09.015.

ZHANG Wenchao1, ZHAO Mengchuan2, FENG Zhishan1, LI Mei1, GUO Yinghui1, LI Guixia1*. The clinical value of pellet agglutination assay for MP antibody titer and colloidal gold method for simultaneously detection of MPIgM and MPIgG in children with mycoplasma pneumonia#br#[J]. Journal of Hebei Medical University, doi: 10.3969/j.issn.10073205.2018.09.015.

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被动颗粒凝集法检测MP抗体效价和胶体金法联合检测MPIgM、MPIgG抗体在儿童支原体肺炎中的应用价值

The clinical value of pellet agglutination assay for MP antibody titer and colloidal gold method for simultaneously detection of MPIgM and MPIgG in children with mycoplasma pneumonia#br#

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