›› 2015, Vol. 36 ›› Issue (2): 170-170.

• 论文 • 上一篇    下一篇

甩片改良抗酸法与 FQ-PCR 技术在脑脊液抗酸菌检查中的应用分析

吕翠环;李玉静;孙志平;李卓;吕莎;郑立恒   

  1. 河北省胸科医院检验科,河北 石家庄,050041%河北省胸科医院骨科,河北 石家庄,050041%河北省胸科医院呼吸科,河北 石家庄,050041
  • 发布日期:2015-02-25

WU Tao;CUI Yun-wei;ZHANG Qi;CHEN Wei;LI Sheng;ZHANG Ying-ze

LV Cui-huan;LI Yu-jing;SUN Zhi-ping;LI Zhuo;LV Sha;ZHENG Li-heng   

  • Published:2015-02-25

摘要: 目的:评价脑脊液改良抗酸染色法与实时荧光定量聚合酶链反应(fluorescence quantitative polymerase chain reaction,FQ-PCR)法诊断结核性脑膜炎(tuberculous meningitis,TBM)的意义。方法 TBM 患者81例,采集脑脊液检测标本,分别采用离心甩片改良抗酸染色法、实时 FQ-PCR 法检测抗酸杆菌。结果81例改良抗酸染色法阳性率66.7%(54/81)高于 FQ-PCR 方法阳性率50.6%(41/81),差异有统计学意义(P <0.05)。结论在诊断 TBM 方面,改良抗酸染色方法显著优于 FQ-PCR 方法。

关键词: 结核, 脑膜, 染色与标记, 实时聚合酶链反应

Abstract: Objective To evaluate united test significance in the diagnosis of tuberculous meningitis (TBM)with modified acid-fast staining method of cerebrospinal fluid and real-time fluorescence quantitative polymerase chain reaction (FQ-PCR).Methods Cerebrospinal fluid samples from 81 patients with TBM were detected for Mycobacterium tuberculosis respectively with centrifugal modified acid-fast staining method and real-time FQ-PCR.Results The positive rate of modified acid-fast staining 66.7%(54/81)was significantly higher than that of FQ-PCR 50.6%(41/81),there was significant difference between the two methods(P <0.05).Conclusion Modified acid-fast staining method is significantly better than FQ-PCR in the diagnosis of TBM.

Key words: tuberculosis, meningeal, staining and labeling, real-time polymerase chain reaction

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