关键词: 无菌培养, 抗药性, 细菌, 微生物敏感性试验

Abstract: Objective To understand the composition of the common pathogens in blood culture and a variety of pathogens to antimicrobial agents in the hospital.To offer the basis for the rational use of antimicrobial drugs to solve the patients'pain.Methods The collection involved 1 764 blood samples.France bioMérieux BacT/Alert3D fully automatic blood culture instrument, supporting medium,identification system were used to identify the pathogens and susceptibility test.Results A total of 1 764 blood samples showed the culture positive rate 10.1%,and there were 179 isolated positive bacteria,among which gram-positive bacteria were 68 (38.0%),gram-negative bacteria 111 (62.0%),at the first place of pathogens as E.coli followed by coagulase-negative bacteria,Klebsiella pneumonia,Pseudomonas aeruginosa.The susceptibility results showed that most of the detected pathogens were resistant to different anti-bacteria agents. Conclusion Clinicians should pay attention to early blood culture of patients,and strictly stick to the rational use of antimicrobial agents based on susceptibility results.

Key words: axenic culture, drug resistance, bacterial, microbial sensitivity tests