河北医科大学学报 ›› 2022, Vol. 43 ›› Issue (1): 12-15,48.doi: 10.3969/j.issn.1007-3205.2022.01.003

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大鼠功能性下颌前伸后髁突软骨骨化前后PLC-γ1的变化研究

  

  1. 1.北京市通州区新华医院口腔正畸科，北京 101100；2.河北医科大学第一医院口腔正畸科，河北 石家庄 050031
  • 出版日期:2022-01-25 发布日期:2022-01-24
  • 作者简介:盛海莹（1985-），女，河北唐山人，北京市通州区新华医院主治医师，医学硕士，从事口腔正畸研究。
  • 基金资助:
    河北省政府资助专科能力建设和专科带头人项目（Ls201801）

Changes of PLC-γ1 cartilage ossification of the posterior condyle of rats after functional mandibular protrusion

  1. 1.Department of Orthodontics, Beijing Tongzhou Xinhua Hospital, Beijing 101100, China; 
    2.Department of Orthodontics, the First Hospital of Hebei Medical University, 
    Shijiazhuang 050031, China
  • Online:2022-01-25 Published:2022-01-24

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摘要： 目的  探讨磷脂酶C-γ1（phospholipase C-γ1，PLC-γ1）在功能性下颌前伸后大鼠髁突后部软骨骨化过程中的作用机制。
方法  60只4周龄健康雄性Sprague-Dawley（SD）大鼠，适应性饲养1周，建立SD大鼠下颌前伸模型，随机等量分为2组，实验组24 h/d 佩戴自制下颌前伸斜面导板矫治器，对照组不作处理。2组大鼠分别于戴用矫治器第1、3、7、14、21、28天时采用断颈法处死5只，取双侧髁突，常规固定、脱钙、脱水、包埋。石蜡切片采用HE染色观察其组织形态学变化,采用免疫组织化学方法结合图像分析检测PLC-γ1在髁突软骨后部的表达。
结果  第7、14、21、28天时实验组髁突软骨后部PLC-γ1表达的强度-色调-饱和度（intensity-hue-saturation，IHS）值高于对照组，差异有统计学意义（P＜0.05）；2组不同时间点PLC-γ1表达的IHS值差异无统计学意义（P＞0.05）。
结论  PLC-γ1可能参与了功能性下颌前伸大鼠髁突软骨骨化的过程，检测其水平对髁突部软骨骨化的研究具有重要参考价值。


关键词: 下颌疾病, 软骨骨化, 磷脂酶C-γ1 

Abstract: Objective  To explore the mechanism of phospholipase C-γ1(PLC-γ1) in cartilage ossification of the posterior condyle of rats after functional mandibular protrusion. 
Methods  Sixty four-week-old male Sprague-Dawley(SD) rats were adaptively fed for one week. The mandibular protrusion model of SD rats was established, and then the rats were divided into experimental group and control group equally and randomly. The experimental group was treated with self-made inclined plane appliance for mandibular protrusion(24 h /d), while the control group was not treated. Five rats in each group were sacrificed at 1, 3, 7, 14, 21, 28 d respectively after using inclined plane appliance. The bilateral condyles were fixed, decalcified, dehyded, and then embedded. The histological changes of the paraffin sections were observed by HE staining, and the expression of PLC-γ1 in posterior condylar cartilage was detected by immunohistochemistry combined with image analysis. 
Results  The IHS value of PLC-γ1 expression in the posterior condyle cartilage of the experimental group was higher than that in the control group at 7, 14, 21, 28 d, and the difference was statistically significant(P＜0.05). The IHS value of PLC-γ1 expression was not significantly different between two groups at different time points(P＞0.05). 
Conclusion  PLC-γ1 may be involved in the process of condylar cartilage ossification of rats after functional mandibular protrusion, and the detection of its level has important reference value for the study of cartilage ossification in condylar process.


Key words: mandibular diseases, cartilage ossification, phospholipase C-γ1

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