河北医科大学学报 ›› 2022, Vol. 43 ›› Issue (3): 326-330.doi: 10.3969/j.issn.1007-3205.2022.03.016

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MCM7在甲状腺乳头状癌组织中的表达及与survivin和P27蛋白的关系分析

  

  1. 广东省佛山市第一人民医院病理科，广东 佛山 528000
  • 出版日期:2022-03-25 发布日期:2022-04-14
  • 作者简介:张鑫（1978-），男，山西长治人，广东省佛山市第一人民医院主治医师，医学硕士，从事病理学与病理生理学研究。
  • 基金资助:
    广东省佛山市卫生健康局医学科研课题计划（20210196）

Expression of MCM7 in papillary thyroid carcinoma and its relationship with survivin and P27 protein

  1. Department of Pathology, the First People′s Hospital of Foshan City, Guangdong Province, Foshan 528000, China
  • Online:2022-03-25 Published:2022-04-14

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摘要：

目的 分析微小染色体维持蛋白7（minichro-mosome maintenance protein7，MCM7）、survivin和P27蛋白在甲状腺乳头状癌组织中的表达及MCM7与survivin、P27蛋白的相关性。

方法 选取我院外科手术切除的60例甲状腺乳头状腺癌、40例甲状腺腺瘤、40例结节性甲状腺肿病理组织及20例甲状腺正常的死亡尸体解剖的甲状腺组织，采用免疫组织化学法检测MCM7、survivin、P27蛋白的表达。分析甲状腺乳头状癌组织MCM7、survivin、P27蛋白表达与患者临床资料的关系及MCM7与survivin、P27蛋白的相关性。

结果 甲状腺乳头状癌MCM7、survivin阳性表达明显高于其他组织，P27蛋白阳性表达明显低于其他组织（P＜0.05）；肿瘤直径>1 cm、TNM分期Ⅲ~Ⅳ、淋巴结有转移的MCM7阳性表达率明显高于肿瘤直径≤1 cm、TNM分期Ⅰ~Ⅱ、淋巴结无转移，TNM分期Ⅲ~Ⅳ、淋巴结有转移的survivin阳性表达率明显高于TNM分期Ⅰ~Ⅱ、淋巴结无转移（P＜0.05）；甲状腺乳头状腺癌组织MCM7表达与survivin表达呈明显正相关（r=0.695,P＜0.05），与P27蛋白表达呈明显负相关（r=-0.508，P＜0.05）；ROC曲线分析显示，MCM7诊断甲状腺乳头状腺癌的AUC为0.942，敏感度为93.3%，特异度为89%.0。

结论 甲状腺乳头状腺癌组织MCM7过表达，与survivin、P27蛋白密切相关，可能共同参与癌组织的发生、发展，促进转移。MCM7可用于鉴别诊断甲状腺乳头状腺癌，具有较高的准确性。

关键词:

甲状腺肿瘤, 微小染色体维持蛋白类, 存活素

Abstract:

Objective To analyze the expression of microchromosome maintenance protein 7(MCM7), survivin and P27 protein in papillary thyroid carcinoma tissues and the correlation of MCM7 with survivin and P27 protein.

Methods Surgically resected tissues of 60 cases of thyroid papillary adenocarcinoma, 40 cases of thyroid adenoma, and 40 cases of nodular goiter that were pathologically confirmed, and thyroid tissue from 20 bodies with normal thyroid gland obtained by biopsy in our hospital were selected. Immunohistochemistry was used to detect the expression of MCM7, survivin, and P27 protein. The relationship between the expression of MCM7, survivin, and P27 protein in papillary thyroid carcinoma tissues and the clinical data of patients, and the correlation between MCM7, survivin, and P27 protein were analyzed.

Results The positive expression of MCM7 and survivin in papillary thyroid carcinoma was significantly higher than that of other tissues, and the expression of P27-positive protein was significantly lower than that in other tissues(P＜0.05). The MCM7 positive expression rate was significantly higher in patients with tumor diameter＞1 cm, TNM stage Ⅲ-Ⅳ, and lymph node metastasis than that in patients with tumor diameter ≤1 cm, TNM stages Ⅰ-Ⅱ, and no lymph node metastasis; the positive expression rate of survivin was significantly higher in patients with TNM stages Ⅲ-Ⅳ and lymph node metastasis than in patients with TNM stages Ⅰ-Ⅱ and no lymph node metastasis(P＜0.05). The expression of MCM7 in papillary thyroid adenocarcinoma tissue was significantly positively correlated with the expression of survivin, and was significantly negatively correlated with the expression of P27 protein(P＜0.05). Receiver operating characteristic(ROC) curve analysis showed that the area under the ROC curve(AUC) of MCM7 in the diagnosis of papillary thyroid adenocarcinoma was 0.942, the sensitivity was 93.3%, and the specificity was 89.0%.

Conclusion The overexpression of MCM7 in papillary thyroid adenocarcinoma tissue is closely related to survivin and P27 proteins, which may participate in the occurrence and development of cancer tissues and promote metastasis. MCM7 can be used for differential diagnosis of papillary thyroid carcinoma, with high accuracy.

Key words: thyroid neoplasms, minichromosome maintenance 1 protein, survivin

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