河北医科大学学报 ›› 2022, Vol. 43 ›› Issue (6): 627-631.doi: 10.3969/j.issn.1007-3205.2022.06.002

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基于PI3K/Akt通路探究下调miR-206对肋骨骨折模型大鼠骨折愈合的影响

  

  1. 1.河北省张家口市第二医院麻醉科，河北 张家口 075000；2.河北省张家口市第二医院骨科，河北 张家口 075000
  • 出版日期:2022-06-25 发布日期:2022-06-28
  • 作者简介:王强（1985-），男，河北张家口人，河北省张家口市第二医院主治医师，医学学士，从事临床麻醉学研究。
  • 基金资助:
    河北省医学科学研究课题计划（20201604）

Effects of down-regulation of miR-206 on fracture healing of rat rib fracture model via PI3K/Akt signaling pathway

  1. 1.Department of Anesthesiology, the Second Hospital of Zhangjiakou, Hebei Province, 
    Zhangjiakou 075000, China； 2.Department of Orthopedics, the Second Hospital of 
    Zhangjiakou， Hebei Province, Zhangjiakou 075000, China
  • Online:2022-06-25 Published:2022-06-28

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摘要： 目的 观察miR-206与PI3K/Akt在大鼠肋骨骨折愈合中的作用。
方法 32只SPF级SD大鼠进行肋骨骨折的造模，后随机分为miR-206组和对照组，每组16只。14 d和28 d每组各处死8只大鼠，行micro-CT检查，观察骨折愈合情况，qPCR检测PI3K和Akt的表达水平，western blot检测PI3K和Akt蛋白水平。
结果 术后大鼠基本状况良好且均造模成功。micro-CT三维重建分析表明，各组大鼠的骨痂总体积、骨性骨痂体积、骨骼体积比均随时间逐渐增加，在14、28 d时，miR-206组在骨痂形成相关指标与对照组的差异有统计学意义(P＜0.05)。qPCR和Western blot结果显示在14、28 d时，miR-206抑制剂注射能明显升高PI3K和AKt的表达量和蛋白水平，差异有统计学意义（P＜0.05）。
结论 下调miR-206能够激活PI3K/Akt通路促进肋骨骨折部位的骨痂生长和骨化过程，从而促进骨折愈合。


关键词: 肋骨骨折, miR-206；PI3K；AKt

Abstract: Objective To observe the effects of miR-206 and PI3K/Akt in rib fracture healing in rats. 
Methods Thirty-two SPF SD rats were used for construction of rib fracture model, and then randomly divided into miR-206 group(n=16) and control group(n=16). Eight rats were sacrificed in each group at 14 d and 28 d. Micro-CT was performed to observe fracture healing. The expression levels of PI3K and Akt were detected by qPCR, and the protein levels of PI3K and Akt were detected by Western blot. 
Results The rats were basically in good condition after the operation, and all the rats were successfully modeled. The micro-CT three-dimensional reconstruction analysis showed that the total callus volume, bone volume, and bone volume/ tissue volume(BV/TV) of rats in each group gradually increased over time. At 14 d and 28 d, there were significant differences in callus formation-related indexes between the miR-206 group and the control group(P＜0.05). The results of qPCR and Western blot showed that miR-206 inhibitor injection could significantly increase the expression and protein levels of PI3K and Akt at 14 d and 28 d, and the differences were statistically significant(P＜0.05). 
Conclusion  Down-regulation of miR-206 can activate the PI3K/Akt pathway to promote the growth and ossification of the callus at the rib fracture site, thereby promoting fracture healing.


Key words: rib fractures, miR-206, PI3K, Akt

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