河北医科大学学报 ›› 2023, Vol. 44 ›› Issue (1): 95-99.doi: 10.3969/j.issn.1007-3205.2023.01.019

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线粒体D-Loop区单核苷酸多态性与喉癌发病风险关系的研究

  

  1. 1.河北省邯郸市眼科医院耳鼻咽喉科，河北 邯郸 056000；2.河北医科大学第四医院急诊科，河北 石家庄 050011；
    3.河北医科大学第四医院耳鼻咽喉头颈外科，河北 石家庄050011

  • 出版日期:2023-01-25 发布日期:2023-01-17
  • 作者简介:程洪坤(1976-)，女，河北临漳人，河北省邯郸市眼科医院副主任医师，医学学士，从事耳鼻喉科疾病诊治研究。
  • 基金资助:
    河北省医学科学研究重点课题计划(20190720)

Study on the relationship between single-nucleotide polymorphisms in mitochondrial D-Loop region and the risk of laryngeal carcinoma

  1. 1.Department of Otorhinolaryngology, the Eye Hospital of Handan City, Hebei Province, Handan 
    056000, China; 2.Department of Emergency, the Fourth Hospital of Hebei Medical University, 
    Shijiazhuang 050011, China; 3.Department of Otorhinolaryngology Head and Neck Surgery, 
    the Fourth Hospital of Hebei Medical University, Shijiazhuang 050011, China

  • Online:2023-01-25 Published:2023-01-17

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摘要： 目的  探讨线粒体D-环区（D-Loop区）单核苷酸多态性（single nucleotide polymorphisms, SNPs）与喉癌发病风险的关系。
方法  实验标本取自河北医科大学第四医院耳鼻咽喉-头颈外科行喉癌手术的患者71 例及在该院体检中心行健康查体的人群159例。采用聚合酶链反应（polymerase chain reaction，PCR）扩增目的片段，并进行线粒体D-Loop区测序。采用χ2检验分析不同SNPs与喉癌发病风险之间的关系，并分析与喉癌发病相关的 SNPs与患者临床特征的关系。
结果  线粒体DNA（mtDNA）D-Loop区的SNPs与喉癌的发病风险有关，73G、309C、315C及16319G基因型可能增加了喉癌的发病风险，而524C、556A、16288T、16291C及16311T基因型可能降低了喉癌的发病风险。
结论  线粒体D-Loop区SNPs可作为预测因子，用于提高喉鳞状细胞癌患者的早诊率，指导喉鳞状细胞癌患者的临床治疗。


关键词: 喉肿瘤, 多态性,单核苷酸, DNA,线粒体

Abstract: Objective  To investigate the relationship between single-nucleotide polymorphisms (SNPs) in the mitochondrial DNA (mtDNA) D-Loop region and the risk of laryngeal carcinoma. 
Methods  Experimental samples were collected from 71 laryngeal carcinoma patients who underwent surgery in the Department of Otolaryngology Head and Neck Surgery at the Fourth Hospital of Hebei Medical University. Samples were also collected from 159 healthy people who underwent physical examination in Medical Examination Center of our hospital. The target fragment was amplified by polymerase chain reaction (PCR), and the mtDNA D-Loop region was sequenced. The χ2 test was used to analyze the relationship between different SNPs and the risk of laryngeal carcinoma, and the relationship between the SNPs associated with laryngeal carcinoma and the clinical features of these patients was also analyzed. 
Results  SNPs in the mtDNA D-Loop region were related with the isk of laryngeal carcinoma. The genotypes of 73G, 309C, 315C and 16319G were significantly related with an increased risk in laryngeal carcinoma, whereas the genotypes of 524C, 556A, 16288T, 16291C and 16311T were associated with the lower risk of laryngeal 
carcinoma. 
Conclusion  SNPs in the mtDNA D-Loopregion can be used as predictors to improve the early diagnosis rate of laryngeal squamous cell carcinoma and guide the clinical treatment of the disease.

Key words: laryngeal neoplasms, polymorphism, single nucleotide, DNA, mitochondrial

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