关键词: 脓毒症, 炎症因子, 单核细胞

Abstract: Objective To investigate the expression of galactose lectin 1 (Galectin-1)/recombinant receptor activity modifying protein (RAMP) in patients with sepsis and the molecular mechanism of regulating inflammatory signaling pathway. 
Methods A total of 80 outpatients and inpatients with sepsis treated in the First Hospital of Hebei Medical University were selected, and 80 healthy volunteers were collected as controls. The expression levels of serum anti-inflammatory molecules Galectin-1 and RAMP in the two groups were detected by ELISA. In terms of cytological experiment, CRISPR-Cas9 technique was used to knock out the galectin-1 gene of monocytes. Monocytes TCP-1 were divided into three groups: control group, NC group (transfected with NC plasmid) and experimental group (transfected with crispr-cas9 plasmid). The expression levels of inflammatory factors interleukin 6 (IL-6), interleukin 8 (IL-8) and interleukin 10 (IL-10) were detected. The expression levels of inflammatory pathway molecules ID3 and RAMP mRNA were detected by qPCR, and the expression levels of ID3 and RAMP proteins were detected by Western blot. 
Results Compared with the control group, the expression levels of serum Galectin-1 and RAMP in sepsis group were higher (P＜0.01). In terms of cytological test, compared with the control group, the secretion of inflammatory factors in the knockout group was increased (P＜0.01). QPCR results showed that compared with the control group, the expression levels of ID3 and RAMP mRNA were increased in the knockout group (P＜0.01), and Western blot results showed that compared with the control group, the expression levels of ID3 and RAMP protein were increased in the knockout group (P＜0.01). 
Conclusion Galectin-1 may affect the secretion and metabolism of inflammatory factors in monocytes by inhibiting the inflammatory signal ID3/RAMP pathway, and participate in the occurrence and development of sepsis.

Key words: sepsis, inflammatory factors, monocyte