呼吸道合胞病毒下呼吸道感染患儿外周血miR-93-5p表达与Th1/Th2平衡的关系

doi:10.3969/j.issn.1007-3205.2023.07.017

河北医科大学学报 ›› 2023, Vol. 44 ›› Issue (7): 836-839,850.doi: 10.3969/j.issn.1007-3205.2023.07.017

呼吸道合胞病毒下呼吸道感染患儿外周血miR-93-5p表达与Th1/Th2平衡的关系

南华大学附属长沙中心医院儿科，湖南长沙 410000

出版日期:2023-07-25 发布日期:2023-07-24
作者简介:周莲花（1983-），女，湖南宁乡人，南华大学附属长沙中心医院副主任医师，医学学士，从事儿科呼吸系统疾病诊治研究。
基金资助:
湖南省科技创新计划项目（S2019JJKWLH0176）

Relationship between Mir-93-5P expression and Th1/Th2 balance in peripheral blood of children with RSV lower respiratory tract infection

Department of Pediatrics, Changsha Central Hospital Affiliated to South China University, Hu′nan Province, Changsha 410000, China

Online:2023-07-25 Published:2023-07-24

摘要/Abstract

摘要： 目的呼吸道合胞病毒（respiratorysyncytialvirus，RSV）下呼吸道感染患儿外周血微小RNA-93-5p表达与辅助型T细胞1/2（Th1/Th2）平衡的关系。
方法选取儿科建档就诊的RSV下呼吸道感染患儿58例为RSV组，同时选取年龄性别匹配的健康儿童60例为对照组，采用实时荧光定量聚合酶链反应（polymerase chain reaction，PCR）检测血清miR-93-5p水平；流式细胞仪计数Th1、Th2百分率，计算Th1/Th2表达，酶联免疫吸附法检测白细胞介素2（ interleukin 2，IL-2）、IL-4、IL-10及干扰素γ（ interferon γ，IFN-γ）水平，Spearman分析miR-93-5p与Th1/Th2的关系；Logistics回归分析影响RSV下呼吸道感染发生的危险因素。
结果 RSV组miR-93-5p水平高于对照组（P＜0.05）；RSV组Th1、Th1/Th2低于对照组（P＜0.05），RSV组Th2高于对照组；RSV组IL-2、IFN-γ水平低于对照组（P＜0.05）；RSV组IL-4、IL-10水平高于对照组（P＜0.05）；RSV组miR-93-5p与IL-2、IFN-γ、Th1/Th2比值呈负相关性（P＜0.05）；与IL-4、IL-10呈正相关性（P＜0.05）；Logistics回归分析显示高水平miR-93-5p与低水平的Th1/Th2是影响RSV下呼吸道感染的危险因素（P＜0.05）。
结论 RSV下呼吸道感染患儿外周血miR-93-5p呈高表达，其高表达可能与Th1/Th2平衡密切相关。

关键词: 呼吸道合胞病毒, 呼吸道感染, miR-93-5p, Th1/Th2平衡

Abstract: Objective To study the relationship between the expression of microrNA-93-5P and T-helper 1 (Th1)/T-helper 2 (Th2) balance in peripheral blood of children with respiratory syncytial virus (RSV) lower respiratory tract infection.
Methods A total of 58 children with RSV lower respiratory tract infection who were registered and treated in the Department of Pediatrics of our hospital were selected as the RSV group, and 60 age- and gender-matched healthy children were selected as the control group. The serum miR-93-5p level was detected by real-time quantitative polymerase chain reaction (RT-qPCR). The percentage of Th1 and Th2 were counted by flow cytometry, and the expression of Th1/Th2 was calculated. The levels of interleukin (IL)-2, IL-4, IL-10 and interferon-γ (IFN-γ) were detected by enzyme linked immunosorbent assay (ELISA), and the relationship between miR-93-5p and Th1/Th2 was analyzed by Spearman. Logistics regression analysis was performed to analyze the risk factors of RSV lower respiratory tract infection.
Results The level of miR-93-5p in RSV group was higher than that in control group (P＜0.05). Th1 and Th1/Th2 in RSV group were lower than those in control group (P＜0.05), while Th2 in RSV group was higher than that in control group; the levels of IL-2 and IFN-γ were lower in RSV group than in the control group (P＜0.05). The levels of IL-4 and IL-10 in RSV group were higher than those in control group (P＜0.05). miR-93-5p inRSV group was negatively correlated with IL-2, IFN-γ and Th1/Th2 ratio (P＜0.05), and positively correlated with IL-4 and IL-10 (P＜0.05). Logistic regression analysis showed that high level of miR-93-5p and low level of Th1/Th2 were risk factors for RSV lower respiratory tract infection (P＜0.05).
Conclusion Mir-93-5P is highly expressed in peripheral blood of children with RSV lower respiratory tract infection, and its high expression may be closely related to Th1/Th2 balance.

Key words: respiratory syncytial viruses, respiratory tract infections, MiR-93-5 p, Th1/Th2 balance

周莲花, 刘麟, 余庆乐, 吴蕾. 呼吸道合胞病毒下呼吸道感染患儿外周血miR-93-5p表达与Th1/Th2平衡的关系 [J]. 河北医科大学学报, 2023, 44(7): 836-839,850.

ZHOU Lian-hua, LIU Lin, YU Qing-le, WU Lei. Relationship between Mir-93-5P expression and Th1/Th2 balance in peripheral blood of children with RSV lower respiratory tract infection[J]. Journal of Hebei Medical University, 2023, 44(7): 836-839,850.

[1]	王丽霞, 王运昌, 李荣荣, 张洁莉, 安静, 李妤蓉. ICU呼吸道铜绿假单胞菌耐药性及其耐药基因分型研究[J]. 河北医科大学学报, 2021, 42(6): 708-712.
[2]	贺晓娇. 不同雾化方式结合振动排痰仪对老年下呼吸道感染患者的治疗效果观察[J]. 河北医科大学学报, 2020, 41(6): 680-685.
[3]	冯志山1，李贵霞1,2，郭映辉2，杨〓硕1，严小桐1，王〓乐1*. 儿童肺炎支原体感染血清学与分子生物学检测方法的对比研究[J]. 河北医科大学学报, 2019, 40(7): 829-833.
[4]	王黎一1，曹旭华2*，史利克1，王〓悦1. 下呼吸道鲍曼不动杆菌感染患者对亚胺培南、美罗培南和头孢哌酮舒巴坦耐药的易感因素分析[J]. 河北医科大学学报, 2019, 40(3): 332-338.
[5]	弭〓莉. 唐山地区呼吸道感染患儿呼吸道病毒检测结果与空气污染相关性研究[J]. 河北医科大学学报, 2018, 38(3): 343-346.
[6]	李继红，时东彦*，袁〓玥，李志荣. 2 253株鲍曼不动杆菌耐药监测及临床价值评估[J]. 河北医科大学学报, 2017, 37(12): 1439-1442,1447.
[7]	李树义 1,鲍承贤 2,张敬 3. 呼吸道感染细菌联合疫苗体内实验研究[J]. 河北医科大学学报, 2016, 37(6): 693-696.
[8]	邸红芹1，王晓玲2（综述），王〓亮3，张〓辉4*（审校）. 呼吸道病原体分子诊断技术研究进展[J]. 河北医科大学学报, 2016, 37(12): 1520-1523.
[9]	邸红芹，杨永辉，康丽菲，安晓颖，李晓霞，张〓辉. 多种呼吸道病原体的多重实时荧光定量PCR检测方法的建立及应用[J]. 河北医科大学学报, 2016, 37(11): 1302-1306.
[10]	韩〓柱1，李惠芬2，李静亚3，王树梅4，杨会义3，吴志华2. 西药引入蒲地蓝口服液治疗上呼吸道感染效果观察[J]. 河北医科大学学报, 2016, 37(11): 1324-1327.
[11]	张亚兰 1,王建利 2,王静利 3,武晨 1. 肺炎支原体特异性 IgG 、 IgM 检测在呼吸道感染中的临床意义[J]. 河北医科大学学报, 2016, 37(1): 97-99.
[12]	郑毅. 集束干预护理在RICU多重耐药鲍曼不动杆菌感染患者中的应用[J]. , 2015, 36(1): 99-99.
[13]	石先伦;钱大东. 老年患者气管插管全身麻醉后下呼吸道感染相关因素分析及预防[J]. , 2014, 35(12): 1455-1455.
[14]	张光辉;时建林;崔华民;马朋羽;颜如玉;宋铁鹰. 感冒评分在合并上呼吸道感染患儿全身麻醉中的应用[J]. , 2013, 34(3): 348-348.
[15]	孟宪萍;王欣颖;郑秀环. 加味三拗汤纳米贴对下呼吸道感染患儿血清TNF-α、IL-1β及IL-13水平的影响[J]. , 2012, 33(2): 209-209.

呼吸道合胞病毒下呼吸道感染患儿外周血miR-93-5p表达与Th1/Th2平衡的关系

Relationship between Mir-93-5P expression and Th1/Th2 balance in peripheral blood of children with RSV lower respiratory tract infection

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