河北医科大学学报 ›› 2024, Vol. 45 ›› Issue (10): 1120-1128.doi: 10.3969/j.issn.1007-3205.2024.10.001

• •    下一篇

慢性间歇性低压低氧对发育大鼠心肌细胞中的L型钙通道的影响

  

  1. 1.河北省石家庄市第三医院医疗美容科，河北 石家庄 050051；2.河北医科大学基础医学院生理学教研室，河北 
    石家庄 050017； 3.河北省人民医院眼科，河北 石家庄 050057；4.河北省唐山市开平区
    疾病预防控制中心健康教育科，河北 唐山 063022

  • 出版日期:2024-10-25 发布日期:2024-10-15
  • 作者简介:许双巧（1986-），女，河北石家庄人，河北省石家庄市第三医院主管护师，医学硕士，从事医疗美容专业研究。
  • 基金资助:
    国家自然科学基金项目（30572086）；河北省医学科学研究课题重点科技研究计划（20200857）

Effect of chronic intermittent hypobaric hypoxia on L-type Ca2+ channels in cardiomyocytes of developing rats

  1. 1.Department of Medical Aesthetics, the Third Hospital of Shijiazhuang City, Hebei Province, 
    Shijiazhuang 050051, China; 2.Department of Physiology, School of Basic Medical Sciences, 
    Hebei Medical University, Shijiazhuang 050017, China; 3.Department of Ophthalmology, 
    Hebei General Hospital, Shijiazhuang 050057, China; 4.Department of Health 
    Education, Centre for Disease Control and Prevention of Kaiping District, 
    Hebei Province, Tangshan 063022, China

  • Online:2024-10-25 Published:2024-10-15

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摘要： 目的 探讨慢性间歇性低压低氧（chronic intermittent hypobaric hypoxia,CIHH）对发育大鼠L型钙电流（L-type calcium current,ICaL）的影响。
方法 新生雄性SD大鼠随机被分为：28 d CIHH干预组（CIHH28）、42 d CIHH干预组（CIHH42）、28 d对照实验组（CON28）和42 d对照实验组（CON42）。其中CIHH干预组分别在模拟3 000 m海拔高度低压低氧的条件下（PB= 525 mmHg，PO2= 108.8 mmHg，5 h/d）（1 mmHg=0.133 kPa）干预28 d和42 d。采用全细胞膜片钳技术记录心室肌细胞中的ICaL。
结果 CON和CIHH组心肌细胞 ICaL的峰值电流和电流-电压（I-V）关系曲线差异无统计学意义（P＞0.05）。在模拟缺血-复灌（ischemia-reperfusion,I/R）过程中，ICaL峰值降低，I-V曲线向上偏移（P＜0.05），但CIHH组心肌细胞中ICaL的变化明显小于CON心肌细胞（P＜0.05）。 CIHH组的ICaL稳态激活曲线和稳态失活曲线在I/R前后均无显著性变化（P＞0.05）。在I/R过程中，CON28和CON42组的 ICaL稳态失活曲线向左偏移（P＜0.05），而CIHH28和CIHH42组心肌细胞没有变化（P＞0.05）。
结论 CIHH可减轻缺血再灌注损伤对发育大鼠心室肌细胞ICaL的影响。


关键词: 慢性间歇性低压低氧, L型钙电流, 心肌细胞

Abstract: Objective To investigate the effects of chronic intermittent hypobaric hypoxia (CIHH) on L-type calcium current (ICaL) in developing rats. 
Methods Newborn male SD rats were randomly divided into 28-day CIHH intervention group (CIHH28), 42-day CIHH intervention group (CIHH42), 28-day control group (CON28) and 42-day control group (CON42). The CIHH intervention group underwent exposure to hypobaric hypoxia at an altitude of 3 000 meters for a duration of 28 days and 42 days respectively, with each session lasting 5 h per day. These conditions were characterized by a partial oxygen pressure (PO2) of 108.8 mmHg （1 mmHg=0.133 kPa） and a barometric pressure (PB) of 525 mmHg. The whole-cell patch clamp technique was employed to record the ICaL in ventricular myocytes. 
Results No significant differences was observed in the peak current or the current-voltage (I-V) relationship curve of ICaLin cardiomyocytes between the CON group and CIHH group (P＞0.05). However, during simulated ischemia/reperfusion (I/R), the peak ICaL was observed to be decreased, accompanied by an upward shift in the I-V relationship curve(P＜0.05).But the change of ICaL in cardiomyocytes was significantly smaller in CIHH group than in CON group (P＜0.05). The steady-state activation and inactivation curves of ICaL in the CIHH group showed no significant changes before and after I/R (P＞0.05). However, during the process of I/R, the steady-state inactivation curve for ICaL underwent a shift towards the left in both CON28 and CON42 groups (P＜0.05), whereas no such shift was observed in cardiomyocytes of CIHH28 and CIHH42 groups (P＞0.05). 
Conclusion CIHH can attenuate the effect of I/R injury on ICaL in ventricular myocytes of developing rat. 


Key words: chronic intermittent hypobaric hypoxia, L-type calcium current, cardiomyocytes

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