Journal of Hebei Medical University

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TracingeffectofPKH26-labeledhumanumbilicalcordmesenchymal stemcellsinendometriumofrats

  

  1. 1.Department of Obstetrics and Gynecology, the People′s Hospital of Hebei Province, Shijiazhuang
    050051, China;  2.Department of Gastroenterology, the People′s Hospital of Hebei Province,
    Shijiazhuang 050051, China； 3.Department of Neurosurgery, the People′s Hospital of
    Hebei Province, Shijiazhuang 050051, China；4.Department of Respiration,
    the People′s Hospital of Hebei Province, Shijiazhuang 050051, China
  • Online:2016-03-25 Published:2016-05-11

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Abstract: [
Abstract ] Objective Bylabelingthehumanumbilicalcordmesenchymalstemcells ( UC-
MSCs ) withPKH26 , themigrationofcellinendometriumofratswereobserved.Labelingand
tracingeffectsofPKH26wereevaluated.Methods IsolatedhumanUC-MSCswerelabeledwith
PKH26.Thelabeledcellsweretransplantedtoratsthroughcaudalvein.Thenthedistributionof
cellsinratendometrium wasobservedunderfluorescencemicroscope.Results Therewereno
detectabledifferencesincellmorphologyandgrowthafterPKH26labeling.Andthecellswere
mainlylabeledwithredfluorescenceunderinvertedfluorescence microscopeand were mainly
distributedin epithelialcells and stromalcells , in endometrial glandular epithelium and
mesenchyme.Conclusion Labelingthehuman UC-MSCswithPKH26isasafeandeffective
method , whichcanbeusedinthestudyoftransplantationofstemcells.

Key words:  mesenchymalstemcells , endometrium , cellslabeling