Effect of resveratrol on proliferation of lung cancer A549 cells and the expression of NFκB

doi:10.3969/j.issn.10073205.2016.09.018

Abstract

Abstract: [
Abstract ] Objective Tostudytheeffectofresveratrolonproliferationofhumanlung
cancercelllineA549andtheexpressionofnuclearfactorkappaB ( NF-κB ) .Methods Theeffect
ofresveratrolonthe proliferation of A549 cells was detected by methylthiazolyldiphenyl-
tetrazoliumbromide ( MTT ) method.TheeffectofresveratrolontheexpressionofmRNANF-κB
inA549cellswasdetectedbyreversetranscription-PCR ( RT-PCR ) .EffectofresveratrolonNF-
κBproteinlevelsinlungcancerA549cellswasdetectedbywesternblotting.Results Withthe
increaseoftheconcentrationofresveratrol , thecellgrowthinhibitionratesingroupB , CandD
werehigherthaninthatingroupA.ThecellgrowthinhibitionratesingroupCandD were
higherthanthatingroupB.ThecellgrowthinhibitionratesingroupDwashigherthanthatin
groupC ( P <0.05 ) .Cellgrowthinhibitionrateat24hand48ingroupB , CandDarehigher
thanthatat12h.Cellgrowthinhibitionrateat48hwashigherthanthatat24h ( P <0.05 ) .
A549cellswith100mol
/ Lresveratrolfor48hcansignificantlydownregulatetheexpressionof
NF-kappamRNA.Conclusion Resveratrolcansignificantlyinhibittheproliferationoflung cancerA549cellsandtheexpressionofNF-κB.

Key words: lungneoplasms , resveratrol , apoptosis , NF-kappaB

YAN Jingxia1, GUO Yong2, ZHANG Rui1, GUO Wei3, HUA Guoxiang4, SU Junhua1*. Effect of resveratrol on proliferation of lung cancer A549 cells and the expression of NFκB[J]. Journal of Hebei Medical University, doi: 10.3969/j.issn.10073205.2016.09.018.

[1]	XIAO Hui, HAN Xing, JIN Hong-jie, LIU Xiao. Long-chain noncoding RNA XIST regulates cell proliferation and apoptosis of mesangial cells in lupus nephritis by targeting miR-150-5p [J]. Journal of Hebei Medical University, 2023, 44(7): 760-766.
[2]	DU Shan-shan, YE Shuang, XIE Long-chuan, XIA Ling-yun. Study on the mechanism of miR-124-3p.2 regulating apoptosis and ferroptosis of oral squamous cell carcinoma cells by CMTM6 [J]. Journal of Hebei Medical University, 2023, 44(3): 252-258,278.
[3]	WU Hai-feng, JIA Jian-lei, LI Qian, ZHEN Xiu-li, WEI Ya-nan, NIE Zhao-yan. Resveratrol ameliorates oxidative stress damage of human granulosa cells induced by cyclophosphamide by regulating SIRT1 [J]. Journal of Hebei Medical University, 2022, 43(7): 832-838.
[4]	WANG Yi-gang, HUANG Ting, WANG Jun-zhou, TANG Rong-xing, LI Su, XIONG Yong. Mechanism of metformin inhibiting malignant cell phenotype of hepatocellular carcinoma cells through miR-194-5p/RBM6 pathway [J]. Journal of Hebei Medical University, 2022, 43(12): 1371-1377,1427.
[5]	TONG Ya-kun, ZHAO Dan-dan, AN Guo-jing. Effect of miR-181d-5p expression on proliferation, apoptosis and invasion of cervical cancer cells [J]. Journal of Hebei Medical University, 2022, 43(11): 1250-1254,1260.
[6]	ZHU Ning, MA Xiao-shan, CHEN Chun-li, LI Guo-xian, YANG Xiu-juan, XIE Dong. Study on the mechanism of geniposide inhibiting oxidative stress and apoptosis of Parkinson′s disease model through AMPK/mTOR pathway [J]. Journal of Hebei Medical University, 2021, 42(9): 1008-1015.
[7]	ZHANG Jia-hui, MA Hong-yun, QIN Juan. The effect of silencing miR-26b viaPI3K/Akt pathway on proliferation and apoptosis of placental trophoblastic cells in patients with pregnancy-induced hypertension [J]. Journal of Hebei Medical University, 2021, 42(9): 1063-1067.
[8]	TIAN Shuo, ZHANG Ning, NIU Shu, LI Shen, SU Li, PING Fen. The protective effect of N-acetylcysteine against PM2.5-induced testicular toxicity [J]. Journal of Hebei Medical University, 2021, 42(7): 754-758,778.
[9]	LU Yu-run, TANG Yu-fan. Protective role and mechanism of berberineagainst hypoxia/reoxygenation-induced injury in H9c2 cells via miR-29b [J]. Journal of Hebei Medical University, 2021, 42(3): 256-261,267.
[10]	YU Hong, MA Mei-na, ZHOU Zhen-zhen, GUO Qing-duo, YU Hong-mei. Effects of PKA-PINK1/Parkin signal pathway on apoptosis and pyroptosis of rat cortical astrocytes exposed to oxygen-glucose deprivation/resuscitation [J]. Journal of Hebei Medical University, 2021, 42(2): 129-133,171.
[11]	YAN Shan-shan, ZHAO Fang, XIAO Jian, ZHANG Chun-liang. Effect of resveratrol on pulmonary function and cardiac function in SD rats with chronic obstructive pulmonary disease and pulmonary heart disease [J]. Journal of Hebei Medical University, 2020, 41(8): 882-887.
[12]	WU Chao, YANG Xu. Expression of thioredoxin-2 in anterior capsule of lens and its relationship with apoptosis and oxidative stress in elderly cataract patients [J]. Journal of Hebei Medical University, 2020, 41(7): 801-804,833.
[13]	ZHAO Juan, LI Long*. Down regulation of MCT1 expression inhibits proliferation and promotes apoptosis of Hela cells#br# [J]. Journal of Hebei Medical University, 2020, 41(5): 570-573,578.
[14]	XU Yan-nan, MENG Li, ZHU Yan, YAN Jing, WANG Yan-ling, ZHOU Chen-ming. Effect of dihydroartemisinin on proliferation and apoptosis of human gastric cancer cell line BGC-823 and its mechanisms [J]. Journal of Hebei Medical University, 2020, 41(11): 1245-1250.
[15]	PANG Ya-xiang1, HAN Hua2, YIN Yue1, MA Guo-hua3, NIU Hong-lin4*. Overexpression of miR-140-5p promotes apoptosis and inhibits proliferation of prostate cancer cells#br# [J]. Journal of Hebei Medical University, 2020, 41(1): 7-11.

Effect of resveratrol on proliferation of lung cancer A549 cells and the expression of NFκB

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments