Abstract: ［Abstract］ Objective〖HTSS〗To investigate the effect of down-regulation of monocarboxylate transporter1(MCT1) on the proliferation and apoptosis of Hela cells and its mechanism.
〖WTHZ〗Methods〖HTSS〗HeLa cells were divided into three groups: untransfected group, negative control group and MCT1 siRNA group. The expression of MCT1, Bcl-2 and Bax protein in Hela cells was detected by Western blot, Hela cell proliferation was detected by MTT, Hela cell cycle distribution and apoptosis rate were detected by flow cytometry.
〖WTHZ〗Results〖HTSS〗Compared with the control group, the expression level of MCT1, Bcl-2, Bax protein, cell proliferation, cycle distribution and apoptosis rate of Hela cells in the negative control group had no significant difference(P＞0.05), but the expression level of MCT1 and Bcl-2 protein, cell proliferation activity and the percentage of cells in S phase in the MCT1-siRNA group were significantly reduced, while the expression level of Bax protein and the percentage of cells in S phase were significantly reduced The percentage and apoptosis rate in G0/G1 phase were significantly increased(P＜0.05).
〖WTHZ〗Conclusion〖HTSS〗Down regulation of MCT1 expression can inhibit Hela cell proliferation by inducing cell cycle arrest, and promote Hela cell apoptosis by up regulation of Bax and down regulation of Bcl-2 protein expression.

Key words: uterine cervical neoplasms, MCT1, cell proliferation, apoptosis