Abstract: Objective To investigate the effect of geniposide(GP) on the apoptosis and oxidative stress injury of the Parkinson′s disease model obtained by 1-methyl-4-phenyl 1,2,3,6-tetrahydropyridine(MPP+) induced human neuroblastoma SH-SY5Y cells through Adenosine 5′-monophosphate(AMP)-activated protein kinase/mammalian target of rapamycin(AMPK/mTOR) pathway. 
Methods The SH-SY5Y cells were selected as the research subject, and according to different treatment methods, they were divided into Control group(normal culture), MPP+ group(supplemented with 1 mmol/L MPP+ on the basis of the Control group), GP group(supplemented with 100 μmol/L GP on the basis of the Control group), and MPP++GP group(supplemented with 100 μmol/L GP on the basis of the MPP+ group). Malondialdehyde(MDA), lactate dehydrogenase(LDH) and superoxide dismutase(SOD) kits, flow cytometry and Western blot were used to detect the changes in oxidative stress injury of cells, cell apoptosis and related proteins expression levels of AMPK, mTOR and LC3 in each group. The changes in the related expression levels of the above proteins in MPP+ group and MPP++GP group were detected by Western blot after treatment with AMPK activator or mTOR inhibitor. 
Results Compared with the Control group, the cell apoptosis, LDH activity, MDA content, AMPK phosphorylation level, LC3-Ⅱ/LC3-Ⅰ ratio were increased, while SOD activity and mTOR phosphorylation level were reduced in the MPP+ group(P＜0.01). Compared with the MPP+ group, the cell apoptosis, LDH activity, MDA content, AMPK phosphorylation level, and LC3-Ⅱ/LC3-Ⅰ ratio were reduced, while SOD activity and mTOR phosphorylation level were increased in the MPP++GP group(P＜0.01). After AMPK activator or mTOR inhibitor was added, the AMPK phosphorylation level and LC3-Ⅱ/LC3-Ⅰ ratio were increased, while mTOR phosphorylation level was reduced in the MPP+ group and MPP++GP group. 
Conclusion GP can inhibit MPP+ induced apoptosis of SH-SY5Y cells, oxidative stress injury and autophagy partly by AMPK/mTOR pathway.

Key words: Parkinson disease, cell apoptosis, geniposide