Abstract: Objective  To investigate the therapeutic effect of nerve growth factor(NGF), naloxone combined with erythropoietin(EPO) in the treatment of neonatal hypoxic-ischemic encephalopathy(HIE), and their effects on the levels of programmed cell death 5(PDCD5) and 8-hydroxy-2-deoxyguanosine(8-OHdG).
Methods  A total of 90 cases of neonatal hypoxic ischemic encephalopathy were randomly divided into observation group and control group, 45 cases in each group. The patients in the control group were treated with naloxone combined with NGF, and the patients in the observation group were treated with erythropoietin on the basis of the therapeutic method of the control group. The recovery time of consciousness, convulsion disappearance, muscle tension recovery and reflex recovery were detected and recorded. The neonatal neurobehavioral assessment(NBNA) score, serum PDCD5 and 8-OHdG levels were compared between the two groups. The mental retardation index(MDI) and psychomotor development index(PDI) of the two groups were evaluated after one year of treatment. The incidence of adverse reactions during the treatment of the two groups were recorded.
Results  The total effective rate of the observation group was 91.1%, and that of the control group was 73.3%. The effective rate of the observation group was higher than that of the control group(P＜0.05). The recovery time of consciousness, convulsion disappearance, muscle tension recovery and reflex recovery in the observation group were significantly shorter than those in the control group(P＜0.01). After treatment, the NBNA scores of the two groups were significantly increased(P＜0.01), and the NBNA score of the observation group was significantly higher than that of the control group(P＜0.01). After treatment, PDCD5 and 8-OHdG in the two groups were significantly lower than those before treatment(P＜0.01), while PDCD5 and 8-OHdG in the observation group were significantly lower than those in the control group(P＜0.01). The MDI and PDI of the observation group were higher than those of the control group after 1 year of treatment(P＜0.05). There were no serious adverse reactions in both groups.
Conclusion  NGF combined with naloxone can reduce the expression of PDCD5 and 8-OHDG, which can reduce brain tissue damage and inhibit cell apoptosis, improve neurological function and promote psychological and motor development of children.

Key words: hypoxia ischemia, brain, NGF, naloxone, erythropoietin