Abstract: Objective  To explore the mechanism of macrophage derived exosomes(MDE) inhibiting the invasion and migration of epithelial ovarian cancer(EOC) cells after treatment with tumor necrosis factor-like weak inducer of apoptosis(TWEAK). 
Methods  MDE were collected and explored before and after TWEAK stimulation, and co-cultured with highly metastatic human ovarian cancer cell H08910-PM. The expression of miRNA-7 in MDE, macrophages and co-cultured HO8910-PM cells were detected by real-time PCR. Western blotting was used to detect the expression of epidermal growth factor receptor(EGFR)/threonine kinase(Akt)/extracellular regulated protein kinase(ERK1)/2 signaling pathway in co-cultured HO8910-PM cells. After AntagomiR-7 treatment, the reduced expression of miRNA-7 in macrophages was managed. Exosomes were collected before and after TWEAK treatment to observe the changes of invasion and migration abilities of HO8910-PM cells. 
Results  After TWEAK stimulation, the expression of miRNA-7 in macrophages and their exosomes was increased, and the expression of miRNA-7 in HO8910-PM cells treated by MDE was higher than that in the control group, suggesting significant differences(P<0.05). 
Conclusion  After TWEAK acts on the secretion of macrophages in vivo, miRNA-7 plays an important role in inhibiting EGFR signaling pathway in EOC cells, thereby continuously inhibiting the entire process of invasion.

Key words: ovarian neoplasms, neoplasm invasiveness, macrophages