Abstract: ［Abstract］ Objective〖HTSS〗〓To explore the effects of ATIP3aHMGA2ERK signaling pathway on cisplatin resistance in epithelial ovarian cancer.
〖HTH〗〖WTHZ〗Methods〖HTSS〗〓DDPresistant ovarian cancer cell lines SKOV3/DDP and A2780/DDP were cultured to exponential phase and assigned to control group(C1and C2 Group), ATIP3a group(A1 and A2 Group), siRNAATIP3a group(S1and S2 Group) and siRNAvector group(VC1 and VC2 Group)(n=3), respectively. The cells in A1 and A2 groups were treated with ATIP3a polypeptides(1 nmol/L). The cells in S1 and S2 groups were treated with siRNAATIP3a, but VC1 and VC2 were treated with siRNAvector as control. Then all the cells were treated with DDP for 72 h. The effects of cell proliferation and invasion were assessed by lactate dehydrogenase and transwell assays. The expression in HMGA2 was measured by Realtime PCR and westernblot. Phosphorylation of ERK was also assessed by Westernblot.
〖HTH〗〖WTHZ〗Results〖HTSS〗〓Compared with C1, ATIP3a polypeptides treatment decreased cell proliferation,invasion, mRNA and protein expression of HMGA2, and phosphorylation of ERK in A1 group. Compared with C1 and A1 groups, siRNAATIP3a increased cell proliferation, invasion, mRNA and protein expression of HMGA2, and phosphorylation of ERK in S1 group. The cell proliferation, invasion, mRNA and protein expression of HMGA2, and phosphorylation of ERK in VC1 group were increased compared with A1, but decreased compared with S1. Compared with C2, ATIP3a polypeptides treatment decreased cell proliferation, invasion, mRNA and protein expression of HMGA2, and phosphorylation of ERK in A1 group. Compared with C2 and A2 groups, siRNAATIP3a increased cell proliferation, invasion, mRNA and protein expression of HMGA2, and phosphorylation of ERK in S2 group.The cell proliferation, invasion, HMGA2 expression of mRNA and protein, and phosphorylation of ERK in VC2 group were increased compared with A2, but decreased compared with S2.
〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓The DDPresistant of human epithelial ovarian cancer might be associated with ATIP3aHMGA2ERK signal pathway.

Key words: ovarian neoplasms, cisplatin resistance, ATIP3aHMGA2ERK signal pathway