Journal of Hebei Medical University ›› 2021, Vol. 42 ›› Issue (9): 998-1002.doi: 10.3969/j.issn.1007-3205.2021.09.002

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Effect of different freezing conditions on the freezing preservation of muscle tissue specimens

  

  1. 1.Graduate School of Hebei Medical University, Shijiazhuang 050017, China; 2.Department of 
    Biochemistry and Molecular Biology, Hebei Medical University, Shijiazhuang 050017, China
  • Online:2021-09-25 Published:2021-09-28

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Abstract: Objective To select the optimal freezing method for different muscular tissues by comparing the effect of different muscular tissue sections under three freezing conditions. 
Methods The tissues used in this experiment included skeletal muscle(gastrocnemius), myocardium, and vascular smooth muscle(thoracic aorta). The three freezing conditions were liquid nitrogen immersion, floating on liquid nitrogen, and direct -80 ℃ methods.After sampling, freezing, sectioning and hematoxylin-eosin(HE) staining, the tissue structure morphology was observed under a microscope. 
Results The optimal freezing condition for the skeletal muscle, myocardium and vascular smooth muscle was liquid nitrogen immersion, floating on liquid nitrogen, and liquid nitrogen immersion or floating on liquid nitrogen, respectively. 
Conclusion There are differences in the optimal freezing conditions for different muscular tissues.


Key words: muscles, frozen section, freezing method, condition optimization