Abstract: Objective To evaluate colloidal gold immunochromatography assay(GICA)for rapid detection of carbapenemase-producing Enterobacterales in blood culture. 
Methods In total, 79 strains of Enterobacterales in blood culture from the Second Affiliated Hospital of Xi′an Jiaotong University were collected, including 57 carbapenem-resistant Enterobacterales and 22 carbapenem-sensitive Enterobacterales. According to NG-Test CARBA 5 kit instructions,five main carbapenemases［klebsiella pneumoniae carbapenemase(KPC),new delhi metallo-β-lactamase(NDM),verona integron-encoded metallo-β-lactamase(VIM),imipenemase metallo-β-lactamase(IMP) andoxacillinase48（OXA)-48］ of the tested strains were detected. The carbapenemase gene was confirmed by polymerase chain reaction（PCR） sequencing. 
Results In 57 strains of Carbapenem-resistant Enterobacterales in blood culture, KPC, NDM, IMP, KPC+NDM and NDM+IMP within were detecated 15 min using NG-Test Carba 5. The sensitivity and specificity were 100% and 100% respectively. The sensitivity and specificity of KPC, NDM, IMP, KPC+NDM and NDM+IMP detected by Jinshanchuan carbapenemase resistance detection reagent were 94.74% and 100% respectively. 
Conclusion NG-Test CARBA 5 and Jinshanchuan carbapenemase resistance detection reagents, as efficient and rapid diagnostic methods, can simplify the complex process of clinical detection of carbapenemase.

Key words: immunochromatographic assay, carbapenem-resistant enterobacterales, carbapenemase, NG-test CARBA 5