Abstract: 〗［Abstract］ Objective〖HTSS〗〓To explore the effect Gli expression on growth of esophageal adenocarcinoma cells  through PLK1 and to detect their molecular mechanisms.
〖HTH〗〖WTHZ〗Methods〖HTSS〗〓mRNA and protein expression of Gli1， Gli2 and PLK1 were detected after 24 h treatment of DMSO，  GANT61, NShh and GANT61&NShh respective by real time fluorescence quantitative PCR and Western blot assay. Cell cycle and apoptosis were measured by flow cytometry after 72 h treatment of GANT61.
〖HTH〗〖WTHZ〗Results〖HTSS〗〓Compared with DMSO group,realtime fiuorescent quantitative PCR and Western blotting showed that GANT61 could downregulate mRNA and protein expression of Gli1, Gli2and PLK1,while NShh could increase mRNA and protein expression of Gli1, Gli2and PLK1 significantly. GANT61&NShh could restore partly mRNA and protein expression of Gli1,Gli2 and PLK1. OE33 cells showed a strong G2/M arrest and apoptosis 72 h after treatment of GANT61(P＜001). Expression of Gli1 mRNA and Gli1 protein, Gli2 mRNA and Gli1 protein ,PLK1 mRNA and Gli1 in GANT61 group were lower than those in DMSO group. Those indexes were higher in Nshh group than in DMSO group and GANT61 group, GANT61+NShh group were lower than DMSO group, but higher than GANT61 group. Proliferation rate of G0/G1 stage, S stage and G2/M stage in GANT61 group were higher than DMSO group, and it was the same trend with 72 h apoptotic rate between GANT61 group and DMSO group.
〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓Glihas crosstalk relationship with PLK1 which may regulate cell cycle and apoptosis. They may play orchestral role in the growth of esophageal  adenocarcinoma.

Key words: esophageal neoplasms, gliomaassociated oncogene homolog, Pololike kinase 1