Journal of Hebei Medical University

Previous Articles     Next Articles

Protective effect of Salvia miltiorrhiza extract on tumor with myocardial ischemic injury

  

  1. 1.Department of Central Laboratory, Affiliated Hospital of Hebei University, Key Laboratory for
    Fractionation Mechanisms and Procedures, Hebei Province, Baoding 071000, China; 2.Department of
    Biochemistry and Molecular Biology, the School of Basic Medical Sciences, Hebei Medical University,
    Shijiazhuang 050017, China； 3.Department of Endocrinology, the Second Hospital of Hebei
    Medical University, Key Laboratory for Hematology of Hebei, Shijiazhuang 050000, China;
    4.Department of Hematology, the Second Hospital of Hebei Medical
    University, Shijiazhuang 050000, China
  • Online:2019-06-25 Published:2019-06-19

PDF (PC)

119

Abstract: ［Abstract］ Objective〖HTSS〗〓To observe the role of Salvia miltiorrhiza extract in tumor with myocardial ischemia model.
〖HTH〗〖WTHZ〗Methods〖HTSS〗〓Thirty female BALB/Cnu nude mice which were 4-6 week old were subcutaneously inoculated with breast cancer MCF7 cells to prepare transplanted tumors. Eighteen mice with successful transplanted tumors were randomly divided into three groups: tumor model group，tanshinone ⅡA group and salvianolic acid B group. After 7 days of transplantation, each group was injected with isoproterenol 2.5 mg/kg intraperitoneally for 7 days to establish an animal model of tumor with myocardial ischemia. Another 6 rats were set as normal control group. The tumor tissue was removed and weighed, and the heart tissue was subjected to Masson′s Trichrome staining. The expression of CD34 and von Willebrand Factor(vWF) in myocardium and tumor tissues was detected by immunohistochemistry. The effect of tanshinone ⅡA on human umbilical vein endothelial cells(HUVECs) and breast cancer MCF7 were observed by MTT.
〖HTH〗〖WTHZ〗Results〖HTSS〗〓The results showed that the area of myocardial collagen fibers in the tumor model group was significantly higher than that in the normal control group. The tumor weight and myocardial collagen fiber area in the tanshinone ⅡA group and salvianolic acid B group was significantly smaller than that in the the tumor model group(P＜005). In the myocardial tissue, the expression levels of CD34 and vWF protein in the tanshinone ⅡA group and the salvianolic acid B group were significantly higher than those in the tumor model group(P＜005) . In the tumor tissue, the expression levels of CD34 and vWF protein in tanshinone ⅡA group and in salvianolic acid B were significantly lower than those in the tumor model group(P＜005). In vitro experiments showed that tanshinone ⅡA can promote the proliferation of HUVECc and inhibit the proliferation of breast cancer cells in a dosedependent manner.
〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓The effect of tanshinone ⅡA on protecting myocardial ischemia and inhibiting the growth of breast cancer cells may be related to angiogenesis.

Key words: myocardial ischemia, breast neoplasms, Salvia miltiorrhiza extract