Abstract: ［Abstract］ Objective〖HTSS〗〓To establish a mouse breast cancer cell line 4T1 with high TTP (trisetetraprolin) expression and to observe the effect of TTP on breast cancer cell line 4T1 apoptosis.
〖HTH〗〖WTHZ〗Methods〖HTSS〗〓The breast cancer 4T1 cell line was stably transfected with the plasmids of PCR3.1 and PCR3.1/mTTP by Lipofectamine 2000. The cell apoptosis was detected using flow cytometry technology.
〖HTH〗〖WTHZ〗Results〖HTSS〗〓Higher mTTP expression was detected in 4T1 cells that has been transfected with PCR3.1+mTTP plasmid using real time quantitative PCR. The apoptosis rate among 4T1, 4T1 PCR3.1 and 4T1 PCR3.1+mTTP was not significant difference. But after treatment the cells with Staurosporine, a apoptosis promoting medicine, for 6 hours, apoptosis rate of 4T1 PCR3.1+mTTP cell line was increased. The apoptosis rate of 4T1, 4T1 PCR3.1 and 4T1 PCR3.1+mTTP was 17%, 18% and 29.5%, respectively.
〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓mTTP could not enhance 4T1 cell apoptosis directly, but it could increase the sensitivity of 4T1 to apoptosis inducing medicine.

Key words: breast neoplasms, apoptosis, transfection