Study on the variation and correlation of regulatory T cells in peripheral blood and serum TGFβ and IL10 in patients with gynecological tumor

doi:10.3969/j.issn.10073205.2016.11.017

Abstract

Abstract: [
Abstract ] Objective ToexploretheexpressionofregulatoryTcells ( Treg ) andTcell
subgroupinperipheralbloodandthevariationandcorrelationoftransforminggrowthfactor-
β
( TGF-
β ) andinterleukin-10 (
IL-10 ) cytokinesinseruminpatientswithgynecologicalcancer.
Methods Ninety-sevencases ( 32caseswithcervicalcancer , 35caseswithendometrialcancer , 30
caseswithovariancancer ) ofpatientswithgynecologicalcancerand30casesofhealthycontrols
wereobtainedasresearchobjects.FlowcytometrywasusedtodetectCD4
+ CD25 + Foxp3 +
and
CD4
+ CD25 + CD127 low regulatoryTcellsandthedistributionofTcellsubgroupinperipheral
bloodofallthesubjects.ELISA wasusedtodetectthecontentofTGF-
β 1 、 TGF- β 2andIL-10 cytokinesinserum.Results Comparedwithhealthycontrolgroup , CD4
+ CD25 +
Foxp3
+
and
CD4
+ CD25 + CD127 low
regulatoryTcellsaccountedfortheproportionofCD4
+
Tcellsexpression
inpatientswithgynecologicalcancergroupinperipheralbloodweresignificantlyhigher ( P <
0.05 ) .TherewasnostatisticalsignificantdifferenceinTcellsubgroupproportion ( P >0.05 ) .
ThecontentsofTGF-
β 1andIL-10inserum weresignificantlyhigher (
P <0.05 ), thecontentof
TGF-
β 2wassignificantlylower (
P <0.05 ) .TheproportionofCD4
+ CD25 + Foxp3 +
andCD4
+
CD25
+ CD127 low regulatoryTcellsaccountedforCD4 + TcellsinperipheralbloodandTGF-
β 1 、
TGF-
β 2andIL-10in serum expressionin patients with gynecologicalcancer group were
significantlylowerinonemonthaftertheoperation , andafterfourcyclesofchemotherapy ( P <
0.05 ), CD4
+ 、
CD8
+ TcellsandCD4 + /
CD8
+ inonemonthaftertheoperation 、
CD4
+ 、
CD19
+ T
cellsafterfourcyclesofchemotherapyweresignificantlylowerthanthatbeforetheoperation
(
P <0.05 ) .TherewaspositivecorrelationbetweenCD4
+ CD25 + Foxp3 + TregandTGF-
β 1 (
r s =
0.220 , P =0.034 ) .TherewerepositivecorrelationbetweenCD4
+ CD25 +
CD127
low regulatoryT
cellsandthecontentofTGF-
β 1 、 TGF- β 2andIL-10 (
r s =0.284 , 0.385 , 0.336 , P <0.01 ) .
Conclusion Theimmunetolerance mechanisminpatients withgynecologycancer mightbe
relatedtotheratioofCD4
+ CD25 + Foxp3 + TregandCD4 + CD25 + CD127 low
Tregaccountedtothe
proportionofCD4
+
Tcells , andtothecontentofTGF-
β 1 、
TGF-
β 2andIL-10cytokines.

Key words: genitalneoplasms, female , T-lymphocytes, regulatory , flowcytometry

YU Danjun1, FAN Jing2, HU Yue1, JIANG Li1, CAO Liyan1, MEN Shuai1. Study on the variation and correlation of regulatory T cells in peripheral blood and serum TGFβ and IL10 in patients with gynecological tumor[J]. Journal of Hebei Medical University, doi: 10.3969/j.issn.10073205.2016.11.017.

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Study on the variation and correlation of regulatory T cells in peripheral blood and serum TGFβ and IL10 in patients with gynecological tumor

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