Abstract: Abstract］  Objective〖HTSS〗〓To study the effect of catecholamine(CA) on lung injury in rats with restraint stress.
〖HTH〗〖WTHZ〗Methods〖HTSS〗〓Fifteen adult male SD rats were randomly divided into control group, restraint stress group and restraint+alphamethylptyrosine(AMPT) group, 5 rats in each group. Both plasma norepinephrine(NE), epinephrine(E) and interleukin1 beta(IL1β), interleukin6(IL6) concentration were assayed by enzymelinked immunosorbent assay(ELISA). Hematoxylineosin(HE) staining was used to observe the pathological changes of lung tissue. The protein levels in the bronchoalveolar lavage fluid(BALF) were detected by bicinchoninic acid method.
〖HTH〗〖WTHZ〗Results〖HTSS〗〓Compared with the control group, the plasma NE and E levels in the restraint stress group, restraint+AMPT group were significantly higher than those in the control group. Compared with the restraint stress group, the levels of NE and E in plasma of the restraint+AMPT group were significantly lower than those in restraint stress group(P＜005). Compared with the control group, the levels of IL1β, IL6 in the lung tissue and BALF protein content of the restraint stress group and restraint+AMPT group were significantly increased. Compared with the restraint stress group, the levels of IL1β, IL6 and BALF in the lung tissue of the restraint+AMPT group were significantly decreased, the difference was statistically significant(P＜005).
〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓Catecholamine may be involved in the lung injury induced by restraint stress. AMPT can significantly reduce the lung injury induced by restraint stress.

Key words: lung injury, catecholamine, restraint stress