Abstract: ［Abstract］ Objective〖HTSS〗〓To observe the alterations of endothelial glycocalyx in early septic shock rabbits induced by different pathogenic factors.
〖HTH〗〖WTHZ〗Methods〖HTSS〗〓Thirtysix male New Zealand rabbits were divided into puncture cecal ligation and puncture(CLP) group, the CLP control group, the CLP 30 mL group, the lipopolysaccharide(LPS) group, the LPS control group and the LPS 30 mL group, 6 rabbits in each group according to a random number table. After establishment of rabbit model of LPS and CLP induced septic shock, they were managed accordingly and the clinical indicators and time for model establishment of each group were recorded and compared.Concentrations of syndecan1 in plasma were measured by enzyme linked immunosorbent assay at different time points early after septic model was established. After six hours, the rabbits were sacrificed, and various organs and tissues were harvested. Western blot and immunohistochemistry were used to detect the expression of endothelial glycocalyx.
〖HTH〗〖WTHZ〗Results〖HTSS〗〓The modeling time of CLP group and LPS group was significantly different(P＜005). There were no statistical differencesin white blood cell count and lactic acid between CLP group and LPS group(P＞005), as well as between CLP control group and LPS control group(P＞005). However, significant differences were found between model group and control group. In addition, model group and control group showed significant differences in central venous blood oxygen saturation(P＜005), as well as between LPS group and CLP group(P＜005). Results of plasma markers revealed that the concentration of plasma syndecan1 was significantly higher in CLP group than in LPS group. HE staining revealed less renal damage in LPS group than in CLP group. Immunohistochemical results showed that the expression of syndencan1 which was darker and higher in LPS group than in CLP group. Western blot results indicated that the expressions of syndecan1 in renal tissues in LPS groups were higher than that in CLP groups.
〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓The endothelial glycocalyx was damaged by both LPS and CLP.LPS caused less damage to endothelial glycocalyx than CLP. After fluid resuscitationcombined with norepinephrine, endothelial glycocalyx will be more likely to be partially restored in LPS group in a short term.

Key words: sepsis, glycocalyx, syndecan1