Journal of Hebei Medical University ›› 2022, Vol. 43 ›› Issue (1): 35-38.doi: 10.3969/j.issn.1007-3205.2022.01.008

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A case-control study of GPC-1 exosomes, CD82 exosomes and CA19-9 in the diagnosis of pancreatic cancer

  

  1. 1.Department of Hepatobiliary Surgery, Hebei General Hospital, Shijiazhuang 050051, China; 
    2.Graduate School， Hebei North College，Hebei Province, Zhangjiakou 075000, China; 
    3.Graduate School， Hebei Medical University， Shijiazhuang 050017, China; 
    4.Department of Surgery, Staff Hospital of Handan Iron and Steel Group 
    Limited Company， Hebei Province, Handan 056001, China
  • Online:2022-01-25 Published:2022-01-24

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Abstract: Objective  To study the value of glypican-1(GPC-1)exosomes, cluster of differentiation(CD) 82 exosomes and CA19-9 in the diagnosis of pancreatic duct adenocarcinoma(PDAC). 
Methods  In total, 31 PDAC patients(PDAC group) and 22 chronic pancreatitis(CP) patients(CP group) were selected. Fasting peripheral blood was collected in the early morning, and exosomes were isolated and stained with immunohistochemistry SP method.The ratio of GPC-1 exosomes to CD82 exosomes was determined by flow cytometry, and the content of CA19-9 was determined by electrochemiluminescence immunoassay. 
Results  The expression of CA19-9 and GPC-1 exosomes in PDAC group was higher than that of CP group, and the difference was statistically significant(P＜0.05). No significant difference was found in the expression of CD82 exosomes in the two groups(P＞0.05). The area under ROC curve(AUC) of CA19-9, GPC-1 and the combination of CA19-9 and GPC-1 exosomes in the diagnosis was 0.771, 0.918 and 0.922 respectively; the diagnostic value of GPC-1 exosomes was superior to CA19-9 in PDAC, and the difference was statistically significant(P＜0.05). The diagnostic value of combination of plasma CA19-9 and GPC-1 exosomes was superior to CA19-9 alone in PDAC,and the difference was statistically significant(P＜0.05); but the diagnostic value of combination of plasma CA19-9 and GPC-1 exosomes was not superior to GPC-1 exosomes alone in PDAC, and the difference was no statistically significant(P＞0.05). And there was no correlation between CA19-9 in peripheral blood and GPC-1 exosomes in PDAC(r=0.237, P=0.200). 
Conclusion  The diagnostic value of plasma GPC-1 exosomes in PDAC is superior to that of CA19-9, and combination of the two indicators can improve sensitivity and reduce the misdiagnosis rate.


Key words: pancreatic neoplasms, tumor marker, diagnosis