Expression changes of peroxiredoxin Ⅲ in the myocardium of renal ischemia reperfusion injury model in rats

doi:10.3969/j.issn.10073205.2016.10.013

Abstract

Abstract: [
Abstract ] Objective Toobservetheexpressionofperoxiredoxin Ⅲ ( PrxⅢ ) inrenal
ischemiareperfusioninjurymodelratsandstudytheroleinoxidativestressresponse.Methods
Afterremovaloftherightkidney , theleftrenalarterywithoutinjurywasremoved , andthe
modelofrenalischemiaandreperfusioninjurywasestablished.After24hoursofreperfusion , the
bloodandkidneyweretaken.Theserumbloodureanitrogen ( BUN ) andserumcreatinine ( SCr )
weredetectedwithpicricacid methodandenzymecouplingratemethod , themalondialdehyde
( MDA ) contentinmyocardialtissuewasdeterminedbythiobarbituricacidcolorimetricmethod.
Therenalmorphologychangewasobservedby HEstaining.ThePrxⅢ mRNA andprotein
expressioninmyocardiumwereevaluatedbyreversetranscriptionpolymerasechainreaction ( RT-
PCR ) andwesternblot.Results Comparedwiththecontrolgroup , theBUNandSCrinserum ofrenalischemiareperfusioninjurygroupweresignificantlyincreased.TheMDAcontent , the
PrxⅢ mRNArelativeexpressionlevelandproteinrelativeexpressionlevelin myocardium of
renalischemiareperfusioninjurygroup werehigherthanthatofcontrolgroup , there were
statisticallysignificantdifference ( P <0.05 ) .Conclusion Afterrenalischemiareperfusion
injury , myocardialtissueappearedoxidativedamage.PrxⅢplaysaroleofantioxidativestressin
thehearttissueofratswithrenalischemiareperfusioninjury.

Key words: reperfusioninjury , kidney , peroxiredoxinⅢ , rats

HUO Hongchang1, WANG Qie2, WANG Suling3, WANG Lei2*. Expression changes of peroxiredoxin Ⅲ in the myocardium of renal ischemia reperfusion injury model in rats[J]. Journal of Hebei Medical University, doi: 10.3969/j.issn.10073205.2016.10.013.

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Expression changes of peroxiredoxin Ⅲ in the myocardium of renal ischemia reperfusion injury model in rats

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