Abstract: To study the protective effect of noninvasive delayed limb ischemia preconditioning（NDLIP） on cerebral ischemia reperfusion injury in rats.
〖HTH〗〖WTHZ〗Methods〖HTSS〗〓Thirty healthy male SD rats randomly divided into 3 groups, Shamoperated group, ischemicreperfusion group and NDLIP group(n=10/group). Rats in NDLIP group were subjected to 5 minutes ischemia/5 minutes reperfusion on the left hind limb once a day for 3 consecutive days, then all rats were subjected to cerebral ischemia reperfusion injury. The Shamoperated group gave the same anesthesia and operation, but did not carry out the occlusion of the common carotid artery. The shamoperated group and ischemicreperfusion group were not performed NDLIP operation. The expression of superoxide dismutase(SOD) and malondialdehyde(MDA) was detected by the kit. The protein expression of Bax, Bcl2 and Caspase3 were detected by Western blotting.
〖HTH〗〖WTHZ〗Results〖HTSS〗〓Compared with the rats in the Shamoperation group, the contents of the hippocampal MDA, Bax and Casaspe3 were significantly higher in the ischemicreperfusion group. Compared with the rats in the ischemicreperfusion group, the contents of the hippocampal MDA, Bax and Casaspe3 were significantly decreased in the NDLIP group. Compared with the rats in the Shamoperation group, the contents of the hippocampal SOD and Bcl2 were significantly decreased in the ischemicreperfusion group. Compared with the rats in the ischemicreperfusion group, the contents of the hippocampal SOD and Bcl2 were significantly increased in the NDLIP group.
〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓NDLIP could significantly reduce the occurrence of apoptosis and oxidative stress damage after cerebral ischemia reperfusion injury, it has protective effect on cerebral ischemia reperfusion injury.

Key words: brain ischemia, reperfusion injury； limb ischemia preconditioning, rats