Abstract: ［Abstract］Objective〖HTSS〗To compare the clinical efficacy of BACTEC MGIT 960 drug sensitivity method(BD960), bacteriophage biological amplification(pha B) and fluorescence quantitative PCR probe melting curve method in detecting drug resistance of Mycobacterium tuberculosis, and to evaluate its application value.
〖HTH〗〖WTHZ〗Methods〖HTSS〗The clinical isolates were identified as the positive clinical isolates of Mycobacterium tuberculosis. The results of the three methods were compared and analyzed by using the BD960, pha B and PCR at the same time.
〖HTH〗〖WTHZ〗Results〖HTSS〗The average reporting time of pha B method and PCR method was significantly shorter than that of BD960 method, and PCR method was significantly shorter than that of pha B method, and the difference was statistically significant (P＜0.05). At the same time, the resistance of 80 clinical isolates were detected by streptomycin, isoniazid, rifampicin and ethambutol, and there was no significant difference between the three methods(P＞0.05). BD960 method was used as the control, the resistance of streptomycin(SM), isoniazid(INH) and rifampin(RFP) by phaB was highly consistent, and that of ethambutol（EMB） was moderate. The results of PCR resistance to INH and RFP were highly consistent and that of SM and EMB were moderate.
〖HTH〗〖WTHZ〗Conclusion〖HTSS〗The resistance of Mycobacterium tuberculosis to first-line antituberculosis drugs can be quickly screened by fluorescence quantitative PCR probe melting curve and bacteriophage biological amplification.

Key words: mycobacterium tuberculosis, drug resistance, comparative study