Abstract: ［Abstract］ Objective〖HTSS〗〓To test expressions of targeting protein for Xklp2(TPX2) in gastric cancer and adjacent normal tissues, also chemosensitivity to cisplatin(CDDP), oxaliplatin(LOHP), to investigate drug resistant related proteins, and to explore the effect of TPX2 in drug resistance of platinumbased drugs in gastric cancer.
〖HTH〗〖WTHZ〗Methods〖HTSS〗〓The fresh tissues and paraffin specimens of 85 cases gastric cancer, 30 cases of adjacent normal tissues were collected. Tissues of cases gastric cancer were divided into chemotherapy group, nonchemotherapy group, and adjacent normal tissues group. Expressions of TPX2 and Pglycoprotein(Pgp), glutathione S transferaseπ(GSTπ), B lymphatic leukaemia 2(Bcl2) were tested with immunohistochemistry. MTT assay was applied to detect chemosensitivity of fresh tissues in each group to CDDP, LOHP.
〖HTH〗〖WTHZ〗Results〖HTSS〗〓MTT assay showed that inhibition rates of CDDP, LOHP to chemotherapy group, nonchemotherapy group were lower than adjacent normal tissues group, and inhibition rates of CDDP, LOHP to chemotherapy group were lower than nonchemotherapy group(P＜005). Inhibition rates of CDDP, LOHP to positive TPX2 group were lower than negative TPX2 group(P＜005). Positive rates of TPX2, Pgp, GSTπ, Bcl2 proteins in chemotherapy group, nonchemotherapy group were higher than that of adjacent normal tissues, and positive rates of these proteins in chemotherapy group were higher than that in nonchemotherapy group(P＜005).
〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓The TPX2 protein might be involved in drug resistance of gastric cancer by regulating some drug resistance related proteins, and expression of TPX2 changed in the process of chemotherapy.

Key words: stomach neoplasms, drug resistance, neoplasm, multidrug resistanceassociated proteins