Abstract: Objective  To investigate the relationship between breast, ovary, uterus ultrasound, bone age and sexual development in adolescent girls. 
Methods  A retrospective analysis of 110 cases of girls who were treated to evaluate puberty development was conducted. The height, weight, bone age film, sex hormone, pelvic ultrasound and breast ultrasound were performed. According to the breast ultrasound grading and clinical Tanner stage, the growth and development parameters related to breast ultrasound grading were analyzed, and the differences among the five ultrasonic grading parameters were evaluated The girls in stage Ⅱ were divided into three subgroups according to breast ultrasound, and the girls with grade B breast ultrasound were also divided into three subgroups according to Tanner stage. 
Results  Breast ultrasound grading was positively correlated with age, bone age, uterine volume, uterine length and ovarian volume(mean volume of left and right ovaries), but not with FSH. According to breast ultrasound, 110 girls were divided into Grade A(20.9%), grade B 46(41.8%), grade C 18(16.4%), grade D 5(4.5%), and grade E 18(16.4%). The age, bone age, BMI, uterine volume, uterine length diameter and ovarian volume of group C, D and E were higher than or larger than those of group A and group B. the level of E2 in Group E was higher than that in group A and group B, and the level of LH in group D and E was higher than that in group A and group B(P＜0.05). Among the 110 subjects, 60(54.5%) were clinical Tanner stage Ⅱ girls. According to the breast ultrasound classification, 15 cases(25.0%) were grade A, 38 cases(63.3%) were grade B, and 7 cases(11.7%) were grade C. There were significant differences in E2, uterine volume, uterine length and ovarian volume among the three groups(P＜0.05), and the above parameters gradually increased with the increase of ultrasonic grading. There was no significant difference in age, bone age, LH, FSH and BMI(P＞0.05). According to Tanner stage, there were 5 cases(10.9%) in stage Ⅰ, 36 cases(78.2%) in stage Ⅱ and 5 cases(10.9%) in stage Ⅲ. There were no significant differences in age, E2, LH, FSH, BMI, uterine volume, uterine length diameter and ovarian volume among the three groups(P＞0.05). The bone age of Tanner stage Ⅲ group was greater than that of Tanner stage I group, and the difference was statistically significant(P＜0.05). 
Conclusion  There is a significant positive correlation between breast ultrasound grading and bone age, uterine volume, uterine length diameter, ovarian volume, which is more accurate and objective for the evaluation of puberty breast development of girls.

Key words: Adolescent Development, breast growth and development, ultrasound