Abstract: Objective To analyze the HBV genotype, distribution of drug-resistant mutation sites and drug resistance of chronichepatitis B(CHB) patients treated with various nucleoside(acid) analogues in Shijiazhuang area. 
Methods A total of 1 157 patients with CHB were selected, and their basic data were collected. Genotyping and gene resistance mutation sites of the patients were detected by gene sequencing technology, and the results were statistically analyzed. 
Results Genotypes of CHB patients in Shijiazhuang were mainly C-type and a small number were B-type. There was no statistical significance in the distribution proportion of HBV genotypes B and C in CHB patients in Shijiazhuang area(P＞0.05). There were statistically significant difference in the distribution proportion of HBV genotypes B and C among different ages(P＜0.05). The HBV genotype C was the highest in patients aged 41 to 60 years. There were 172 cases with no mutation, 393 cases with single mutation, 361 cases with 2 gene loci mutation,174 cases with 3 gene loci mutation, 42 cases with 4 gene loci mutation, 10 cases with 5 gene loci mutation, 3 cases with 6 gene loci mutation, 1 case with 7 gene loci mutation and 1 case with 9 gene loci mutation. There was no significant difference in genotype distribution with the proportion of B and HBV genotype C(P＞0.05). A total of 1 889 mutation loci were detected in 985 patients with mutations. Among the 14 gene loci detected, the mutation rates of M204I/V/S, L180M and A181V/T/S were the top three mutation loci. There was no significant difference in the proportion of HBV genotypes B and C among different loci(P＞0.05). In 985 patients with mutations, a total of 11 nucleoside(acid) resistance patterns were detected for lamivudine(LAM), telbivudine(LDT), adefovir(ADV) and entecavir(ETV). There were three patterns of resistance to a single drug, four patterns of resistance to two drugs, three patterns of resistance to three drugs, and one pattern of resistance to all four drugs. There was no statistical significance in the proportion of HBV genotypes B and C among patients with different drug resistance patterns(P＞0.05). 
Conclusion The genotypes of CHB patients in Shijiazhuang area are mainly type C, and the gene resistance mutations are mainly multi-drug resistance.

Key words: hepatitis B virus, genotyping, drug resistance, mutation