Abstract: Objective To explore the effect of laparoscopic excision of giant ovarian cystson ovarian function, inflammatory factors and T lymphocyte subsets in patients. 
Methods A total of 76 patients with giant ovarian cysts treated in our hospital were prospectively randomly selected. They were divided into control group and observation group according to random number table method, with 38 patients in each group. The control group received traditional open surgery for the removal of giant ovarian cysts, and the observation group underwent laparoscopic removal of ovarian giant cysts. The perioperative related indicators, stress indicators, ovarian function, inflammatory factors, T lymphocyte subsets and postoperative complications were compared between the two groups. 
Results The duration of operation and the length of hospitalization in the observation group were shorter than those in the control group, and the blood loss of the observation group was less than that of the control group (P＜0.05). After operation, cortisol (Cor), adrenaline (A) levels and visual analogue scale (VAS) scores were increased in the two groups, which, however, were lower in the observation group than in the control group (P＜0.05). After operation, the levels of luteinizing hormone (LH) and follicle stimulating hormone (FSH) in the two groups were increased, but the levels were lower in the observation group than in the control group (P＜0.05). After operation, the levels of high-sensitivity C-reactive protein (hs-CRP), interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) in the two groups were increased, but the levels in the observation group was lower than those in the control group (P＜0.05). After operation, the levels of CD3+, CD4+/CD8+ in the two groups were decreased, but the levels in the observation group were higher than those in the control group (P＜0.05). The postoperative complications in the observation group were fewer than those in the control group (P＜0.05). 
Conclusion Compared with traditional open surgery, laparoscopic excision of giant ovarian cysts can significantly reduce the stress response of patients, with lower postoperative inflammation, less damage to ovarian function and immune function, fewer postoperative complications, and higher clinical application value. 

Key words: ovarian cysts, ovarian function, T-lymphocyte subsets