Table of Content

25 July 2017, Volume 38 Issue 7

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Effects of mesenchymal stem cells combined with resveratrol on inflammation in mice with experimental autoimmune encephalomyelitis#br#

WANG Dong1, PANG Xiaolu2, WANG Liang1, ZHANG Lihong1, WANG Ye3

2017, 37(7):  745-749.  doi:10.3969/j.issn.10073205.2017.07.001

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〗［Abstract］ Objective〖HTSS〗〓To study in depth the machenism underlying the therapeutic efficiency of mesenchymal stem cell combined with resveratrol in encephalomyelitis（EAE）, and to  investigate the effect of mesenchymal stem cell combined with resveratrol on immune inflammation in EAE mice.
〖HTH〗〖WTHZ〗Methods〖HTSS〗〓We investigate the therapeutic efficiency of bone marrow mesenchymal stem cell combined with resveratrol in experimental autoimmune encephalomyelitis by clinical score. The infliteration of immune cells such as monocytes and lymphocytes into brain was observed using HE staining and immunohistochemistry with antiCD4 antibody. ELISA was conducted to detect the secretion of IFNγ/TNFα and IL4/IL10 inserum.
〖HTH〗〖WTHZ〗Results〖HTSS〗〓The average clinical score, maximal clinical score, CD4+ T cell infiltration, IFNγ, TNFα were lower in mBMMSCs group, resveratrol group, mBMMSCs combined resveratrol group than that in PBS group. The mBMMSCs combined resveratrol treatment group was lower than the mBMMSCs treatment group and the resveratrol treatment group(P＜005); The levels of IL4 and IL10 were higher in mBMMSCs group, resveratrol group, mBMMSCs combined resveratrol group than that in PBS group. Combination treatment group was higher than mBMMSCs group or resveratrol group. The differences were statistically significant(P＜005).
〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓Combination treatment with mBMMSCs and resveratrol can significantly delay the progression of EAE disease.  Combined treatment can reduce the number of infla mmatory cells in EAE mouse spinal cord and promote the transition of type Th1 to type Th2 cytokines in EAE mice.


Effect nicorandil combined with tirofiban on PPCI of noreflow in the patients with acute STsegment elevation myocardial infarction#br#

2017, 37(7):  750-755.  doi:10.3969/j.issn.10073205.2017.07.002

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［Abstract］ Objective〖HTSS〗〓To observe the treatment effects of the targeted coronary intravascular injection of nicorandil combined with tirofiban on noreflow phenomenon(NRP) during primary percutaneous coronary intervention(PPCI)in  patients with acute STelevation myocardial infarction(STEMI).
〖HTH〗〖WTHZ〗Methods〖HTSS〗〓One hundred and twenty patients with acute STEMI were randomly divided into tirofiban group(T group), nicorandil group(N group) and combined medication group(T+N group), each group had 40 cases. When the NRP occurred,T group, N group and T+N group were injected with tirofiban(25 μg/kg), nicorandil(4 mg) and tirofiban(25 μg/kg) plus nicorandil(4 mg) respectively by the micro catheter coronary target vessels. After the operation, 3 groups were injected intravenously tirofiban（0.15 μg·kg-1·min-1）, nicorandil(8 mg/h), tirofiban（0.15 μg·kg-1·min-1）plus nicorandil(8 mg/h) for 24 h, respectively. The thrombolysis in myocardial infarction(TIMI) blood flow grade of infarction related artery, corrected TIMI frame count(cTFC), TIMI myocardial perfusion grade(TMPG) before and at the end of PPCI operations, the STsegment resolution(STR) rate 90 min after operations, creatine kinase isoenzymeMB(CKMB) and cardiac troponinI(cTnI) before operations and those peak volume after operations, nterminal probrain natriuretic peptide(NTproBNP), wall motion score index(WMSI), left ventricular ejection fraction(LVEF), the incidence of intraoperative malignant arrhythmia, hypotension, bleeding and major adverse cardiac events(MACEs) after operations were observed and recorded.
〖HTH〗〖WTHZ〗Results〖HTSS〗〓Compared with N group and T group, the cTFC frames and the peak volume of CKMB and cTnI after PPCI, and NTproBNP and WMSI 1 week after operations in T+N group were significantly decreased(P＜0.05), but the proportion of TIMI 3 grade and TMPG 3 grade after operations, the STR rate 90 min after operations and the LVEF 1 week after operations in T+N group were significantly higher(P＜0.05). There was no significant difference in the above indice between N group and T group(P＞0.05). The incidence of malignant arrhythmia rate in N group or T+N group was lower than that in T group(P＜0.05).
〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓Target coronary intravascular injection of nicorandil combined with tirofiban after NRP occurred can safely and effectively treat the occurrence of NRP during PPCI operation in acute STEMI patients, and improve the level of myocardial perfusion and heart function.


Correlation study between RDW and pulmonary hypertension in patients with chronic obstructive pulmonary disease

WANG Lihong1, YUAN Lindong2, LIU Xiaoyu1, SUN Wuzhuang1*

2017, 37(7):  756-759.  doi:10.3969/j.issn.10073205.2017.07.003

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〗［Abstract］ Objective〖HTSS〗〓To explore the relationship between circulating red cell distribution width(RDW) and the pulmonary arterial hypertension in patients with chronic obstructive pulmonary disease.
〖HTH〗〖WTHZ〗Methods〖HTSS〗〓According to the degree of pulmonary artery pressure, patients are divided into normal pulmon artery pressure group(<35 mmHg, n=56),mild pulmon artery pressure group(35-50 mmHg, n=47), moderate pulmon artery pressure group(＞50-70 mmHg, n=50) and severe pulmon artery pressure group(>70 mmHg, n=45)．Fifty healthy persons served as control group, The RDW and pulmonary function and arterial blood gas analysis were measured and compared.
〖HTH〗〖WTHZ〗Results〖HTSS〗〓The RDW and PaCO2 were increased with the increase of pulmon aryarterial systolic pressure(PASP)(P＜0.05), but PaCO2 had no difference between control group and normal pulmon artery pressure group. The PaO2, forced expiratory volume in one second to pred(FEV1/pred) and forced vital capacity rate of one second(FEV1/FVC) were decreased of with the increase of PASP(P＜0.05). The RDW was significantly positive correlated with pulmonary hypertension(r=0.745， P=0.000). The FEV1％pred, FEV1/FVC, pH, PaO2were negatively correlated with RDW(r=-0.678,-0.696， -0.306， -0.629， P=0.000).
〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓RDW can be used as one of the evaluation indicators of decline in lung function in patients with chronic obstructive pulmonary disease, and acidosis and hypoxemia are also the main reasons for the RDW.


Association between interleukin 28B gene polymorphisms and chronic hepatitis C virus infection and response to antiHCV treatment#br#

KONG Lingbo, NAN Yuemin*, ZHANG Yuguo, ZHAO Suxian, WANG Rongqi, DI Hailing

2017, 37(7):  760-763,769.  doi:10.3969/j.issn.10073205.2017.07.004

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［Abstract］ Objective〖HTSS〗〓To explore the association of interleukin 28B(IL28B) single nucleotide polymorphisms(SNPs) with chronic hepatitis C virus(HCV) infection and response to antiHCV treatment.
〖HTH〗〖WTHZ〗Methods〖HTSS〗〓Two hundred and fiftythree patients with chronic hepatitis C and compensated cirrhosis, and 83 healthy controls were genotyped for the IL28B rs8099917 polymorphisms by TaqMan system. The relation of IL28B rs8099917 SNPs and chronic HCV infection, and the association of IL28B rs8099917 SNPs and virological responses were analyzed. Chisquare test was performed for comparison of categorical data.
〖HTH〗〖WTHZ〗Results〖HTSS〗〓The patients with chronic HCV infection and the healthy controls showed similar frequencies of G, T allele and GG/GT, TT genotypes(P＞005). IL28B rs8099917 SNPs were neither significantly associated with baseline HCV RNA level nor with HCV genotype(P＞005). The presences of IL28B rs8099917 T allele and TT genotype were significantly higher in patients achieved early virological response  and sustained virological response than nonresponse patients.
〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓In Hebei province, polymorphisms in IL28B rs8099917 were not associated with susceptibility to chronic HCV infection. IL28B rs8099917 T allele and TT genotype were predictors for better virological responses.


Expression of HIF1α and correlated target genes in cervical cancer#br#

WANG Fang1, YIN Liyong2*, LOU Juan3, GU Tao1, FU Zhanzhao1

2017, 37(7):  764-769.  doi:10.3969/j.issn.10073205.2017.07.005

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〗［Abstract］  Objective〖HTSS〗〓To evaluate the expression of hypoxia inducible factor1α(HIF1α) , glucose transporter protein1(GLUT1) and vascular endothelial growth factor(VEGF) in cervical cancer and to investigate the relationships between their expressions and the clinicopathological characteristics.
〖HTH〗〖WTHZ〗Methods〖HTSS〗〓The expression levels of HIF1α, GLUT1 and VEGF were detected in 54 cases of cervical cancer by SP immunohistochemieal methods, compared with 10 cases of normal cervical epithelium tissue. The correlation between the expression of HIF1α, GLUT1 and GLUT1，VEGF in cervical cancer was analyzed.
〖HTH〗〖WTHZ〗Results〖HTSS〗〓The positive expression rates of HIF1α, GLUT1 and VEGF in cervical cancer tissues were higher than control group(P＜005). The positive expression rates of HIF1α, GLUT1 and VEGF in cervical cancer with lymph node matastasis were significantly higher than those in no lymph node matastasis group(P＜005). The expression of GLUT1 was positively related to lymph node matastasis, histological grades and clinical stages(P＜005). Positive correlation existed between the expression of HIF1α and GLUT1,VEGF in cervical cancer（rs=0.280, 0.320, P＜005）.
〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓The high expression of HIF1α, GLUT1 and VEGF shows that the invasion of cervical cancer is even higher. Combined detection of the three indicators can be used to accurately assess the prognosis in cervical cancer and evaluate the clinical effects of radiotherapy.


Clinical study on treatment of recurrent spinal tuberculosis with individualized surgical combined with local closed tube irrigation#br#

LI Zhuo， ZHANG Helong， YAO Liming， LIU Fengsheng， DONG Zhaoliang， YAO Xiaowei*

2017, 37(7):  770-773.  doi:10.3969/j.issn.10073205.2017.07.006

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［Abstract］  Objective〖HTSS〗〓To evaluate the clinical efficacy of individualized surgery combined with closed catheter irrigation in the treatment of recurrent spinal tuberculosis.
〖HTH〗〖WTHZ〗Methods〖HTSS〗〓Identified 64 cases of recurrent spinal tuberculosis were given systemic antituberculosis medicine treatment before the individualized surgery combined with continuous localized lavage chemotherapy. Then all patients were treated with antituberculosis drugs for 18 to 24 months after surgery. The clinical symptoms, abscess clearance and bone fusion were observed.
〖HTH〗〖WTHZ〗Results〖HTSS〗〓All surgery was completed successfully and all patients had no intraoperative or postoperative complications. After operation, continuous lavage with amikacin normal saline was given for 14 to 21 d. No sinus, fistula and meningeal infection occurred, and there was no retrograde infection of the drainage tube. The patients were followed up for 24 to 36 months. All achieved perfect intervertebral bone fusion. At the last followup, the VAS score and erythrocyte sedimentation rate were significantly lower than those before surgery(P<0.05), and the JOA score of the spinal function was significantly higher than that before the operation(P<0.05).
〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓The effect of radical debridement combined with catheter irrigation for retreatment of spinal tuberculosis is satisfactory.

Comparative study of ulnar variance measurements by different methods#br#

SUN Cheng, BU Jianli*, WANG Jiangtao,QIAN Yuhang, YIN Yu, CHE Jianwei

2017, 37(7):  774-777,781.  doi:10.3969/j.issn.10073205.2017.07.007

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［Abstract］ Objective〖HTSS〗〓To compare the agreement and reliability of ulnar variance measurements on radiograph, computed tomography(CT), magnetic resonance imaging(MRI).
〖HTH〗〖WTHZ〗Methods〖HTSS〗〓Thirtytwo volunteers who underwent wrists posteroanterior and lateral Xray, Xray, CT and MRI were recruited. The differences in ulnar variance measured with different methods were evaluated with KruskalWallis H analysis of variances. Intraclass correlation coefficient(ICC) was used to assess the repeatability of seven methods, and agreement between raters and methods were compared using BlandAltman plots.
〖HTH〗〖WTHZ〗Results〖HTSS〗〓There was no significant difference in ulnar variance measured by seven methods（P＞005）. Interrater and intrarater ICC were both using excellently in all 7 methods. The CT threedimensional reconstruction was outstanding in reliability and agreement.
〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓PA radiography, lateral radiography, coronal CT multiplanar reconstruction, sagittal CT multiplanar reconstruction, CT threedimensional reconstruction, coronal MRI(bonebone)and coronal MRI(cartilagecartilage), all the methods mentioned above could be used in measuring the UV. CT 3D reconstruction method was the most reliable and consistent method for measuring UV, which was more accurate than other 6 methods for measuring UV.


Application of repeated intravenous medicine combined with intraarticular injection of tranexamic acid in primary hip replacement#br#

BAI Weixia1， LI Haoru2， QIN Di3， HE Liying3， YANG Liu3， LU Lianjun4*

2017, 37(7):  778-781.  doi:10.3969/j.issn.10073205.2017.07.008

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［Abstract］  Objective〖HTSS〗〓To evaluate the outcome of reduplicative intravenous administration combined with intraarticular injection of tranexamic acid(TXA) in primary total hip arthroplasty(THA).
〖HTH〗〖WTHZ〗Methods〖HTSS〗〓Total of 92 patients, accepting primary total hip arthroplasty, were divided into experiment group(intravenous administration of TXA 15 mg/kg before incision, intraarticular injection of TXA 1.0 g intraoperation, intravenous administration of TXA 10 mg/kg 3 hours postoperation, n=46) and, control group (intravenous administration of TXA 15 mg/kg before incision, intraarticular injection of TXA 1.0 g intraoperation, n=46）. Postoperative evaluation index include bleeding volume, transfusion volume and complications as well as variation of hemoglobin and blood volume, erythrocrit, and hospital stays.
〖HTH〗〖WTHZ〗Results〖HTSS〗〓Three days after operation, no deep venous thrombosis(DVT) was found among all patients through ultrasounds. Both experiment group and control group have one patient receving transfusion with the volume of 400 mL. There was no significant difference between the two groups in intraoperative bleeding volume, haematocrit and hospital stay. However significant differences were found in postoperative bleeding volume and hemoglobin content between the two groups(P＜005).
〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓〖JP2〗Postoperative bleeding volume was significantly decreased in primary THA with reduplicative intravenous administration combined with intraarticular injection of TXA.

Study on the efficacy of debridement combined with 420 nm intense pulse light in the treatment of moderate to severe acne and depressed acne scarring and inflammatory erythema#br#

HAN Lanqin, ZHAO Xuelian, ZHANG Chunli, ZHANG Jie, HAN Peng

2017, 37(7):  782-785.  doi:10.3969/j.issn.10073205.2017.07.009

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〗［Abstract］ Objective〖HTSS〗〓 To study the efficacy of debridement combined with 420 nm intense pulsed light in the treatment of the moderate to severe acne and the depressed scar and inflammatory erythema.
〖HTH〗〖WTHZ〗Methods〖HTSS〗〓Two hundred and fiftyfour cases of acne patients were  divided into two groups. One hundred and twentyeight cases as the treatment group were treated with acne debridement and 420 nm intense pulsed light, 126 cases as the control group were treated with 420 nm intense pulsed light. 1 times every 2 weeks, a total of 4 times. After 2 months in the end of treatment，the curative effect, occurrence of depressed scar and regression of inflammatory erythema were evaluated.
〖HTH〗〖WTHZ〗Results〖HTSS〗〓The differences in clinical efficacy were statistically significant（P＜001）. The satisfaction of depressed scar was 92.2% in treatment group and 69.8% in control group, there was a significant difference between the two groups(P＜001). As for the extinction time of inflammatory erythema, the treatment group was also significantly faster than the control group(P＜001).
〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓Debridement combined with 420 nm intense pulsed light in treatment of moderate to severe acne is more effective than 420 nm intense pulsed light alone. The occurrence of epression scar was significantly reduced, while the inflammatory erythema was promoted.


Effect of pulsed dye laser therapy on infantile hemangioma during neonatal period

ZHANG Wenxian1， YANG Yifan1， XUE Lei1， GAO Yang1*， WANG Le2， CAO Minna1

2017, 37(7):  786-788.  doi:10.3969/j.issn.10073205.2017.07.010

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［Abstract］  Objective〖HTSS〗〓To study the feasibility and validity of pulsed dye laser(PDL) for treating infantile hemangionma(IH) in neonates.
〖HTH〗〖WTHZ〗Methods〖HTSS〗〓All patients were diagnosed as infantile hemangionmas and were divided to neonate group(younger than 28days, 93 patients) and nonneonate group(older than 28 days, 1 788 patients). Their clinical data were collected, and the treatment outcomes and PDL parameters in neonates and nonneonates were analyzed.
〖HTH〗〖WTHZ〗Results〖HTSS〗〓The neonates group shows a better therapy result than nonneonatal group(P＜005). Laser energy used per treatment session was signifcantly lower in neonatal group than in nonneonatal group(P＜005). Total laser energy used in neonates was also markedly lower than the control group(P＜005). However, treatment session numbers in these two groups were no significant difference(P＜005).
〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓PDL, with distinct parameters, is a safe, feasible and effective treatment method for the therapy of IH in neonates. After each laser treatment, neonates has a better treatment result and recovered faster than nonneonatal patients.


Effects of doule analgesia instrument combined with labor ball on the delivery outcomes and postnatal depression for primipara#br#

CUI Yuexin, SHEN Wei, SHI Li, GUO Hui, WANG Juan, YIN Jie*

2017, 37(7):  789-792.  doi:10.3969/j.issn.10073205.2017.07.011

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［Abstract］ Objective〖HTSS〗〓To observe the effects of doule analgesia instrument combined with labor ball on the delivery outcomes and postnatal depression for primipara.
〖HTH〗〖WTHZ〗Methods〖HTSS〗〓A total of 598 cases of primipara, according to the admission order and informed consent, were randomly divided into contro group（206 cases, with no any analgesic measures）, DA group(142 cases, pregnant women who used the doule analgesia instrument), LB group(134 cases, pregnant women who used the labor ball ) and DL group(116cases, pregnant women who used the doule analgesia instrument combined with labor ball). Then the total labor time, the amounts of postpartum bleeding in first 2 hours, cesarean section rate, degree of labor pain(visual analogue scale，VAS), neonatal Apgar score, the Edinburgh Postnatal Depression Rating Scale(EPDS) at 7 days postpartum and postpartum incidence of adverse events were observed.
〖HTH〗〖WTHZ〗Results〖HTSS〗〓The total labor time, the amounts of postpartum bleeding in first 2 hours, rate of cesarean section, VAS score, neonatal Apgar score and the EPDS scale in DA group, LB group and DL group were significantly decreased than that of control group(P＜005). And the effects above in DL group were significantly effective than that of DA group and LB group, respectively(P＜005). There was no any postpartum adverse events occurred in every group.
〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓Using of doule analgesia instrument combined with labor ball can effectively shorten the labor time, decrease the postpartum bleedings, reduce the rate of cesarean section, reduce the maternal childbirth pain and prevent the postpartum depression.


Clinical study on the effect of different broken umbilical time on the outcomes of pregnant women and newborns#br#

SUN Yimei1， LIU Yan2，DU Chenguang3

2017, 37(7):  793-796.  doi:10.3969/j.issn.10073205.2017.07.012

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［Abstract］  Objective〖HTSS〗〓To investigate the effects of oxytocin combined with delayed cord clamping and early cutting umbilical cord on maternal and neonatal perinatal health and related complications.
〖HTH〗〖WTHZ〗Methods〖HTSS〗〓Total of 210 cases of cesarean section and neonatal was performed. All these patients were randomly assigned to control group in which umbilical cords were early cut(ECC) and experimental group in which cut umbilical cord more later(DCC). Age, gestational weeks, birth weight, third labor time, postpartum hemorrhage, bleeding and neonatal Apgar score, the occurred rate of postpartum two weeks of neonatal jaundice and anemia were recorded and analyzed.
〖HTH〗〖WTHZ〗Results〖HTSS〗〓Postpartum bleeding volume in DCC group was less, neonatal 2 weeks in peripheral blood hemoglobin was higher and anemia occurred was lower than that in the control group. The difference is significantly different(P＜005). There was no significant difference in general comparison， age, gestational age and birth weight of newborn(P＞005). The third production duration and Apgar score, umbilical cord blood hemoglobin comparison, the incidence of jaundice was not statistically different compare to the control group(P＞005).
〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓The safety period of umbilical cord rupture was 90-120 s for the newborns. The proper delay of breaking the umbilical time and the use of oxytocin can shorten the third stage of labor and reduce the amount of postpartum hemorrhage. It will not increase or aggravate the jaundice of the newborn and reduce the occurrence of anemia.


The protective effects of Astragalus polysaccharide on diabetic rats with retinopathy

LIU Junhui1, LI Chunjiang1, LI Yutao1,2*

2017, 37(7):  797-800.  doi:10.3969/j.issn.10073205.2017.07.013

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［Abstract］  Objective〖HTSS〗〓To study the therapeutic effects of Astragalus polysaccharide(APS) on diabetic rats with retinopathy, and to explore its mechanism.
〖HTH〗〖WTHZ〗Methods〖HTSS〗〓Fortyfive cleaning degree healthy male SD rats were randomly divided into 3 groups, normal control group, diabetic model group and APS group(n=15/group). The diabetic rats were made by streptozotocin intraperitoneal injection. One week after modeling, rats in APS group was administrated orally with APS(400 mg/kg) once daily for consecutive 8 weeks. After treatment, the blood glucose and body weight of different groups were measured. The expression of tumor necrosis factor alpha(TNFα) and intercellular adhesion molecule 1(ICAM1) in retina of different groups were detected by ELISA. The relative expression of vascular endothelial growth factor (VEGF) and pAkt in retina of different groups were tested by western blot.
〖HTH〗〖WTHZ〗Results〖HTSS〗〓The blood glucose of model group and APS group was higher than control group, body weight of model group and APS group was lower than control group(P<0.05). The expression of TNFα, ICAM1, VEGF and pAkt in model group and APS group was higher than that of control group. The expression of TNFα, ICAM1, VEGF and pAkt in APS group was lower than that of model group(P<005).
〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓APS could decrease the expression of TNFα, ICAM1, VEGF and pAkt in retina of diabetic rats. Its protective effect on the retina may be through antiinflammatory effect, reducing Leukocyte adhere to diabetic retina and affecting Akt VEGF signaling pathways.


Effects of combined orthodontic and orthognathic treatment on profile improved and occlusal function of the patients with skeletal Ⅲ malocclusion and mandibular shifting#br#

XU Jianhua， WANG Ling, LIU Huaiqin, XU Yang

2017, 37(7):  801-804.  doi:10.3969/j.issn.10073205.2017.07.014

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［Abstract］ Objective〖HTSS〗〓To investigate the effect of combined orthodontic and orthognathic treatment on profile improved and occlusal function of the patients with skeletal Ⅲ malocclusion and mandibular shifting.
〖HTH〗〖WTHZ〗Methods〖HTSS〗〓The clinical data of 27 patients  were analyzed. All patients received orthodontic treatment combined with orthodontic treatment. We compare the rate of soft tissue asymmetry before and after surgery to evaluate the improvement of the appearance of the patients. We compare muscle surface raise potential and muscle asymmetry index before and after surgery to evaluate occlusal function.
〖HTH〗〖WTHZ〗Results〖HTSS〗〓The Q value of the upper and lower lip deviation 3 months after orthognathic surgery was lower than that of one month before treatment, while the Q value of the bilateral mandibular deviation was higher than that of one month before treatment(P＜005). Three months after treatment, the right masseter muscle recruitment potential, the left and right sides of the abdominal muscle recruitment potential were lower than those of one month before treatment. After 6 months of treatment, the recruitment potential of the left and right temporal muscles, the right and left masseter muscle recruitment potential and the right and left sides of the abdominal muscle of the two groups were significantly higher than those of  one month before treatment and three monthes after treatment(P＜005). Three months after treatment, the asymmetry index of the temporal muscle, the asymmetry index of the masseter muscle and the asymmetry index of the two abdominal muscles were significantly increased, but  those were decreased at six monthes after treatment when compared with a month before the treatment and three monthes after the treatment(P＜005).
〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓The orthodontic treatment combined with orthognathic treatment is very effective. The curative effect is remarkable and stable, and it can meet the requirements of the patients to restore the beauty and the occlusal function. It is worthy of clinical promotion.


Experimental study on PPO gene silencing in human malignant melanoma cell line A375 by RNA interference#br#

WANG Zhengxiang1， ZHANG Guoqiang2， GAO Shunqiang2， ZHAO Guisong3， LIU Siyuan3*

2017, 37(7):  805-809.  doi:10.3969/j.issn.10073205.2017.07.015

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［Abstract］  Objective〖HTSS〗〓To investigate the effect of silencing PPO gene on the growth of human malignant melanoma cell line A375 by RNA interference.
〖HTH〗〖WTHZ〗Methods〖HTSS〗〓 A375 cells were transfected with PPO adenovirus vector, expression of PPO was detectded by RTPCR in the A375 cell, MTT colorimetric analysis method was used to detect the effects on the proliferation of A375 cells, by flow cytometry and Annexin ⅤFITC/PI double staining, changes of early apoptosis were detected.
〖HTH〗〖WTHZ〗Results〖HTSS〗〓 The expression of PPO mRNA was decreased in the transfection group than in the nontransfection group and negative virus group(P＜0.05). After transfected with PPO, the OD values of the three groups at 7 d were higher than that at 1 d, 3 d and 5 d, the OD values of cells at 5 d were higher than at 1 d and 3 d, the OD values of cells at 3 d were higher than at 1 d(P＜0.05). The OD value of transfection group was higher than the nontransfection group and negative virus group（P＜005）. After transfected, the apoptosis rate of the three groups at 7 d was higher than that at 1 d, 3 d and 5 d, the apoptosis rate of cells at 5 d was higher than that at 1 d and 3 d, the apoptosis rate of cells at 3 d was higher than that at 1 d(P＜0.05). The apoptosis rate of transfection group was higher than the nontransfection group and negative virus group（P＜005）.
〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓PPO gene silence can inhibit the proliferation and induce early apoptosis in A375 cells.


Clinicopathological logical and imaging observation of 45 cases of cholesterol pneumonia#br#

KANG Lifei1， ZHU Guiyun1, DI Hongqin2, AN Xiaoying1, LI Xiaoxia1，YANG Yonghui1*

2017, 37(7):  810-813.  doi:10.3969/j.issn.10073205.2017.07.016

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［Abstract］  Objective〖HTSS〗〓To observe and compare the clinicopathologic feature between primary and secondary cholesterol pneumonia, and to discuss the clinical characters in order to providing theoretical basis for understanding and diagnosing diseases.
〖HTH〗〖WTHZ〗Methods〖HTSS〗〓By comparing the imaging of 8 cases of primary cholesterol pneumonia and 37 cases of secondary, combined with the diagnosis under light microscope and the clinical feature , we sum up the characteristics using the statistical analysis.
〖HTH〗〖WTHZ〗Results〖HTSS〗〓Clinical manifestation of primary and secondary cholesterol pneumonia mostly included sputum with blood in phlegm cough, fever, chest condition, etc. No significant difference was found in the two groups(P>0.05). The proportion of women with primary cholesterol pneumonia is higher than that of the secondary, and the difference was statistically significant(P<0.05). The ratio of age over 50 and the position of cholesterol pneumonia were not observed obvious difference in the two groups(P>0.05). The constituent ratio of the imaging, round target character, liquid airfilled bronchi, pleural thickening and mediastinal lymph node enlargement were not found significant difference in the two groups(P>0.05). There was no  statistical significance of the ratio of the mass size which is more than 3cm in the two groups(P>0.05).The foam cells gathered in the alveolar space with cholesterol cleft and multinuclear giant cell under the light scope in primary and secondary cholesterol pneumonia, and the proliferation of lymphocytes was found in the pulmonary interstitium. Also, the cubic alveolar epithelial hyperplasia was observed.
〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓Different types of cholesterol pneumonia cannot be distinguished by clinical and imaging manifestations, of which diagnosis was still subject to pathological diagnosis. The most common was nodular mass type, the accompanying manifestation including the round target character and pleural thickening and the foam cells aggregation in light microscope, which provided theoretical basis for clinical diagnosis and treatment.

The application of detecting serum miRNA206, 155 in hyperthyroidism relapse after relieving#br#

JIANG Yanfeng1， CHEN Anzhi2*， HU Liaoliao3， QI Xuelin4

2017, 37(7):  814-818.  doi:10.3969/j.issn.10073205.2017.07.017

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［Abstract］  Objective〖HTSS〗〓To investigate the clinical application value of detecting serum microRNA206， 155 in predicting hyperthyroidism relapse after drug withdrawal.
〖HTH〗〖WTHZ〗Methods〖HTSS〗〓The expression levels of serum microRNA206，155 in 142 patients with hyperthyroidism were detected by  technology(45 cases in the nontreatment group, 41 cases in the relapse group and 56 cases in the nonrelapse group) in detecting serum T3, T4 and TSH levels by the electrochemical luminescence technology. The inner diameter(diameter, D) and the maximum systolic blood flow velocity(peak systolic velocity, Vmax) of the superior thyroid artery(superior thyroid, STA) were examined by color Doppler ultrasonography.
〖HTH〗〖WTHZ〗Results〖HTSS〗〓In the relapse group and the nontreatment group, the levels of T4,T3, STAD and Vmax were higher than those in the nonrelapse group, respectively, but TSH were lower（P＜005). The levels of T4, T3, TSH, STAD and Vmax between the relapse group and the nontreatment group were compared, but without statistically significant difference（P＞005). The expression of miRNA206 was negatively correlated with the expression of miRNA155(r=-0.692, P＜001). MiRNA206 expression was negatively correlated with T4, T3, STAD and Vmax(r=-0.562, -0.593, -0.537, -0.519, P＜001) and positively correlated with TSH(r=0.622, P＜001). The expression of miRNA155 was positively correlated with serum T4, T3, STAD and Vmax(r=0.530, 0.562, 0.553, 0.503, P＜001), and negatively correlated with TSH(r=-0.689, P＜001). The sensitivity and specificity of miRNA206 and miRNA155 in the diagnosis of hyperthyroidism at the best critical value were 93.2% and 90.7%, 91.6% and 97.2%, respectively.
〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓The detection of serum microRNA206, 155 can be used in prediction of hyperthyroidism relapse after discontinuation of medicine treating, and may be an useful warning markers and intervention targets for hyperthyroidism treating.