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Table of Content

    25 June 2017, Volume 38 Issue 6
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    The effects of Rhodosin on cognition in rats with vascular demedtia
    LIU Na1， XU Ming3， WANG Hualong1， WANG Wenting1，
    2017, 37(6):  621-624,632.  doi:10.3969/j.issn.10073205.2017.06.001
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    ［Abstract］ ObjectiveTo study the effects of Rhodosin on cognition in rats with vascular demedtia(VD), and to explore the mechanisms of its neuprotective effects. MethodsThe VD group rats were established by bilateral common carotid artery occlusion. Cognitive function was tested by the Morris water maze experiment after Rhodosin treatment for 4 weeks. The expression of orgotein superoxide dismutase(SOD) and malondialdehyde(MDA) (oxidative stress indicators) was detected by the kit. The protein expression of p38, pp38 and caspase3 were detected by western blot. Results In VD group, the cognitive ability was declined accompanied by decreased SOD and increased MDA, pp38, caspase3 expression. The cognitive ability in Rhodosin group was significantly enchanced, and Rhodosin  administration reversed the changes of SOD, MDA, pp38 and caspase3 expression accordingly. ConclusionRhodosin could improve the spatial learning and memory ability of VD rats significantly. Its therapeutic mechanisms may be through its antioxidative stress effect and inhibiting caspase3dependent apoptosis via suppressing the p38 MAPK signaling pathway.
    The analysis of clinical effect of berberine combined with atorvastatin on patients with acute #br# cerebral ischemic stroke and the regulating effect on serum inflammatory factors
    WANG Pei, LI Ying, REN Hong, FENG Rui
    2017, 37(6):  625-627,637.  doi:10.3969/j.issn.10073205.2017.06.002
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    ［Abstract］ ObjectiveTo investigate the clinical effect of berberine combined with atorvastatin on patients with acute cerebral ischemic stroke(AIS) and the regulating effect on serum macrophage migration inhibitory factor(MIF), interleukin6(IL6) and monocyte proportion(MONO%). MethodsOne hundred and twenty patients with AIS were recruited and randomly divided into 2 groups: combined group(n=60) and atorvastatin group(n=60). They all received the basical treatment of AIS. Combined group received berberine(300 mg, tid) and atorvastatin(40 mg, qn). Atorvastatin group received atorvastatin(40 mg, qn). The levels of serum MIF, IL6, MONO%(before treatment and after treatment) and the nerve function defect grades(before treatment、after treatment and day 90) were measured and compared. ResultsThe levels of serum MIF, IL6 and MONO% were no significant differences between the two groups before treatment(P＞005). After 14 days treatment, the levels of serum MIF, IL6 and MONO% of the two groups were significantly lower(P＜005). And the levels of serum MIF, IL6 and MONO% of combined group after treatment were significantly lower than those of atorvastatin group(P＜005). The National Institute of Health Stroke Scale(NIHSS) scores was no significant difference between the two groups before treatment(P＞005). After 14 days treatment, the NIHSS scores of the two groups were significantly lower(P＜005). And the NIHSS scores of combined group after treatment were significantly lower than those of atorvastatin group(P＜005). On day 90, the Modified Rankin Scale(mRS) scores of combined group were significantly lower than those of atorvastatin group(P＜005). There was positive correlation of serum MIF, IL6, MONO% and NIHSS scores before treatment(P＜005). ConclusionCombined berberine with atorvastatin can significantly improve neurological impairment and downregulate the levels of serum MIF, IL6 and MONO% of patients with acute cerebral ischemic stroke.
    Association analysis between PNPLA3 gene polymorphism and susceptibility to HBV infection
    LIU Sijing1, GAO Xia2, XUE Peng3, ZHANG Xiaolin2, YANG Lei2， SHEN Yaxin4*
    2017, 37(6):  628-632.  doi:10.3969/j.issn.10073205.2017.06.003
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    ［Abstract］  ObjectiveTo investigate the association of PNPLA3 gene polymorphisms (rs738408, rs738409, rs2294918, rs2294919 and rs2281135) with chronic infection of hepatitis B virus vaccine(HBV). MethodsCase control study was used. For chronic HBV infection in line with the conditions included (case group) 1 792 cases, chronic HBV infection (control group) 618 cases, the association between multiple SNP loci of PNPLA3 gene and susceptibility to HBV infection was analyzed by statistical methods such as   test and multiple Logistic regression analysis. ResultsThe subjects with the PNPLA3 rs738408 TC/TT genotype might have lower risk of HBV infection(OR=0.059, 95% CI=0.028-0.125, P<0.001 for TC; OR=0.020, 95%CI=0.007-0.053, P<0.001 for TT), the subjects with rs738409 GG genotype had a 27.550(95% CI=10.024-75.718, P<0.001) times higher odds of HBV infection. rs2294918 AA genotype might reduce the risk of HBV infection(OR=0.354, 95% CI=0.210-0.599, P<0.001). rs2294919 TC genotype had a 0.598 (95% CI=0.429-0.833， P=0.002) times lower odds of HBV infection. The results mentioned above were adjusted by sex, age, smoke and drink values. ConclusionThere was significant relationship between PNPLA3 gene polymorphisms(rs738408 TC or TT, rs738409 GG, rs2294918 AA and rs2294919 TC) and HBV infection.
    Protective effect of melatonin on renal injury induced by acute paraquat poisoning in rats
    GONG Yu， CHEN Hui， MENG Zhaohua， LYU Baopu， YAO Dongqi， TIAN Yingping
    2017, 37(6):  633-637.  doi:10.3969/j.issn.10073205.2017.06.004
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    ［Abstract］ ObjectiveTo observe the changes of serum urea nitrogen(BUN), creatinine(Cr) in paraquat poisoning rats， and to study the mechanism of paraquatinduced renal injury in rats， the expression changes of transforming growth factorβ1（TGFβ1） to assess the protective effect of melatonin in paraquat poisoning. MethodsNinety adult healthy SpragueDawley(SD) rats were divided into three groups randomly, 30 rats in each group. Control group and poisoned group were treated intragastrically with 1mL of paraquat(50 mg/kg) diluted with normal saline. Control group were treated with the same dose of normal saline as poisoned group and melatonin group. melatonin group were given 1 mL of melatonin at a dose of 10 mg/kg diluted with normal saline(once daily, intraperitoneally). Control and poisoned group were treated with the same dose of normal saline(once daily, intraperitoneally) as melatonin group. Six rats in each group were sacrificed on the 1st, 3rd, 5th, 7th and 14th day after exposure. Blood samples were taken from the heart to observe the changes of plasma BUN and Cr. The expression of TGFβ1 in rat renal tissue was detected by immunohistochemistry. ResultsThe BUN of the rats in the poisoned group was significantly higher than that in the control group at each time point， melatonin group BUN at all time points were lower than the poisoned group，but still higher than the control group. BUN in the poisoned group and melatonin group continued to increase from day 1 to day 5, and gradually decreased on the 7th day, the difference was statistically significant（P＜005）. The Cr concentration in the poisoned group was higher than that in the control group at each time point. Except for the first, 3 days to 14 days in  days group were lower than that in poisoned group(P＜005), but still higher than that in control group, and there was no significant difference between control group at each time point(P＞005). The Cr in the poisoned group and the melatonin group continued to increase from day 1 to day 5, and gradually decreased on the 7th day, the difference was statistically significant（P＜005）. The levels of TGFβ1 in the poisoned group were higher than those in the control group at each time point，melatonin group was lower than that of the control group except the 3rd day, but still higher than the control group, the difference was statistically significant（P＜005）. ConclusionTGFβ1 is involved in the pathogenesis of paraquat poisoning kidney injury. melatonin has protective effect on paraquat poisoning kidney tissue, which might be mediated by TGFβ1 in the paraquat induced renal injury，but its regulation path still need a further exploration．
    The diagnostic value of miRNA92a combined with micro pepsinogen in gastric carcinoma
    NIU Weiwei, YANG Chunchun, DUAN Zhiying, HUO Xiaohui
    2017, 37(6):  638-641,671.  doi:10.3969/j.issn.10073205.2017.06.005
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    Abstract］  ObjectiveTo investigate the value of microRNA92a combined with pepsinogen(PG) in the diagnosis of gastric carcinoma. MethodsSixty cases of gastric cancer, 141 cases of chronic atrophic gastritis and 162 cases of chronic nonatrophic gastritis were adopted，using realtime PCR assay for detection of micro RNA92a and using immune colloidal gold technique for measuring PG. The sensitivity and specificity of miRNA92a, PG and miRNA92a combined with PG in the diagnosis of gastric cancer were calculated respectively. ResultsThe expression of miRNA92a in chronic atrophic gastritis group and non atrophic gastritis group was lower than gastric cancer group. The expression level of plasma miRNA92a in chronic non atrophic gastritis group was lower than chronic atrophic gastritis group(P＜005). The expression level of PG Ⅰ and PG Ⅰ/PGⅡ in gastric cancer group were lower than chronic atrophic gastritis group and chronic non atrophic gastritis group. The value of PG Ⅰ/PGⅡ in chronic atrophic gastritis group was lower than non atrophic gastritis group(P＜005). There was not significant between chronic atrophic gastritis and chronic atrophic gastritis group(P＞005). microRNA92a sensitivity in the diagnosis of gastric cancer and specificity were 85.70% and 70.8%. PG in sensitivity and specificity in the diagnosis of gastric cancer were 75.80% and 84.90%. The sensitivity of micro RNA92a combined with PG diagnosis of gastric cancer and precancerous lesion and the specificity was 86.49%, the specificity was 89.32%. Conclusionmicro RNA92a combined with PG was better than single detection in diagnosis of gastric cancer.
    Analysis of the factors affecting the detection rate of esophageal and gastric mucosa ectopic and the evaluation of the quality of esophageal examination
    PING Fumin， GUO Yongze*， WANG Jing， GU Shuxin
    2017, 37(6):  642-645.  doi:10.3969/j.issn.10073205.2017.06.006
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    Abstract］  ObjectiveTo study the factors affecting endoscopic detection of heterotopic gastric mucosa of the esophagus（HGME）, and to discuss whether HGME is considered as a evaluation index of quality of esophageal endoscopy. MethodsProspective clinical study was performed on patients who received gastroscopy. Research data included endoscopist experience, withdrawal time of the esophagus, sedation endoscopy were recorded.  HGME detection rates and the factors influencing HGME in gastroscopy were analyzed. ResultsA total of 2 000 patients were included in this paper，of which 194 were found to have heterotopic gastric mucosa of the esophagus, the detection rate was 9.7%. The experience level of HGME group was higher than that of non HGME group, and the rate of sedation endoscopy was higher than that of non HGME group, the withdrawal time was longer than that of non HGME group, the difference was statistically significant(P＜005), and the detection rate of HGME was increased with the increase of physician experience. Correlation analysis showed that physician experience was positively related to HGME detection rate(rs=0.685, P=0.001). ConclusionDetection rate of heterotopic gastric mucosa of the esophagus could be regard as an evaluation index of esophagus quality control.
    #br# Effect of Teachback mission pattern on patients with upper gastrointestinal hemorrhage
    REN Haixia1, SHAN Tieqiang2, LI Shuxia1,
    2017, 37(6):  646-649.  doi:10.3969/j.issn.10073205.2017.06.007
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    Abstract］ ObjectiveTo explore the influence of Teachback mission pattern on the patients with upper gastrointestinal hemorrhage. MethodsOne hundred patients with upper gastrointestinal hemorrhage were divided into two groups according to random numbers table: observation group and control group, 50 cases in each group. Control group patients were given the traditional education pattern of nursing. Observation group patients were given Teachback mission pattern of nursing. Patients knowledge level of related upper gastrointestinal hemorrhage, the status of mental health, and satisfaction of nursing work, hospitalization rates again within 30 days and within 90 days out of the hospital are compared between two groups. ResultsThe knowledge rate about health condition in patients of observation group is higher than that of control group after the 5 d in the hospital(P<0.01). The scores in fear, anxiety, depression, paranoia, hostility psychological status of observation group of patients were lower than that of the control group(P＜005). The evaluation satisfaction of nursing work of observation group is higher than that of control group(P＜005). The hospitalization rates again within 30 days and within 90 days out of the hospital of observation group are lower than that of control group(P＜005). ConclusionTeachback mission pattern is superior to the traditional education pattern.
    Effect of predictive nursing on elderly patients with diabetic foot
    WANG Hong1， ZHANG Jie2， NIU Lixia3， WANG Weichao4， HU Yaping5， LIU Fude5
    2017, 37(6):  650-653.  doi:10.3969/j.issn.10073205.2017.06.008
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    Abstract］  ObjectiveTo study the effect of predictive nursing on elderly patients with diabetic foot. MethodsEightytwo cases of elderly patients with diabetes foot were divided into observation group(n=41) and control group(n=41) according to the order of admission. The control group received routine nursing care, and the observation group received predictive nursing on the basis. Diabetes foot knowledge score, foot care behavior score, treatment effect and amputation rate were compared between 2 groups before intervention and after 2 months intervention. ResultsAfter the intervention, diabetic foot awareness score and foot care behavior score were significantly higher than those before intervention. Diabetic foot knowledge score, foot care behavior score in the observation group was significantly higher than the control group(P＜005). The clinical efficacy of the observation group was better than that of the control group. The total effective rate was higher than that of the control group, and the amputation rate was lower than that of the control group(P＜005). ConclusionNursing care of patients with diabetic foot can significantly improve the knowledge score, foot care behavior score and clinical curative effect, and reduce the incidence of deteriorate ,improve the quality of life in patients with diabetic foot, most likely to avoid amputation, which is worthy of clinical attention.
    Clinical study of serum TGFβ1 in nonsmall cell lung cancer before and after radiotherapy
    WANG Jing, JIA Jinghao， YANG Haifang， WANG Zhiwu*
    2017, 37(6):  654-658.  doi:10.3969/j.issn.10073205.2017.06.009
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    ［Abstract］ ObjectiveTo assess the value of serum transforming growth factorβ1(TGFβ1) in predicting the radioactive pneumonia(RP) and the shortterm effect, and to provide a reference for radiotherapy(RT) of nonsmall cell lung cancer(NSCLC). MethodsAn analysis was performed on the medical records of 264 cases with NSCLC. The pathology was confirmed via histology or cytology, using three dimensional conformal radiation therapy. The serum TGFβ1 level was detected before and after RT for all the patients. The TGFβ1 level in serum was detected with enzymelinked immunosorbent assay. Shortterm effect was evaluated according to the WHO unified evaluation criterion after a month of radiotherapy and RP was diagnosed by Radiation Therapy Oncology Group(RTOG) grading standard of acute radiation injury. SPSS 17.0 statistical software was used for statistics. ResultsThe level of serum TGFβ1 preradiotherapy was 442.20-12 840.00 ng/L, the median and the quartile values were 677.5 ng/L and 2 186.7 ng/L. The levels of TGFβ1 postradiotherapy were 280.0-11 753.0 ng/L, median and quartile values were 605.0 ng/L and 1 172.6 ng/L, respectively(Z=-4.617, P=0.000). There were higher incidence of RP in male than female patients, smokers than nonsmokers, PTV>450 cm3 than≤450 cm3, radiotherapy period≤45 d than>45 d(P＜005), in patients with radiotherapy dose<60 Gy, and with increased levels of TGFβ1 after radiotherapy(P＜0.05). Patients with concurrent chemoradiotherapy, radiotherapy dose higher than 60 Gy, serum TGFβ1<677.5 ng/L preradiotherapy preferred to have better response rate(P＜005). Multivariate analysis showed that smoking, PTV>450 cm3, increased serum levels of TGFβ1 after chemotherapy were RP risk factors(P＜005). Patients with concurrent chemotherapy, radiotherapy dose≥60 Gy,  central lung cancer, serum TGFβ1 levels≤677.5 ng/L preradiotherapy had better efficacy(P＜005). ConclusionLower level of TGFβ1 before radiotherapy predicted a better prognosis. Patients with increased serum TGFβ1 levels after radiotherapy had more RP risks. Serum TGFβ1 levels could be considered as a predictor of NSCLC prognosis.
    The application of CTA in preoperative evaluation of laparoscopicassisted radical  gastrectomy
    GAO Yingchao1, CHU Zhifen1, ZHANG Zhikun2, CAI Yannan3, WANG Yuanyuan1, MENG Limin1*
    2017, 37(6):  659-662.  doi:10.3969/j.issn.10073205.2017.06.010
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    Abstract］ ObjectiveTo explore the value of CT angiography(CTA) in the preoperative examination of radical gastrectomy. MethodsFiftyfour patients with gastric cancer who were confirmed by gastroscopy and proposed laparoscopic radical gastrectomy according to the inspection method were divided into two groups: 26 patients with the experimental group and 28 patients with the control group. All patients underwent abdominal CT examination before operation, and the patients in the experimental group were added with volume rendering techniques to generate threedimensional images of the gastric vessels, and all patients underwent laparoscopic radical gastrectomy for gastric cancer(D2). The operation and the number of lymph node dissection were compared between the 2 groups, and the variations of CTA in the peripheral vessels and the peripheral arteries were observed. ResultsIn the experimental group, 26 patients with gastric cancer were treated with laparoscopic radical gastrectomy, CTA for the left gastric artery, right gastroepiploic artery, common hepatic artery, properhepatic artery, and gastroduodenal artery could visualize at 100.0% and the right gastric artery at a percentage of 57.7%, and  left gastroepiploic artery at a percentage of 53.8%, CTA showed celiac axis and hepatic artery variation in 5 cases(19.2%) . The operation time and the amount of bleeding in the experimental group were less than those in the control group, the difference was statistically significant(P＜005). ConclusionFor patients with advanced gastric cancer undergoing laparoscopic surgery, preoperative CTA examination can accurately show the gastric vessels and mutation, tumor surrounding tissue relationship and degree of invasion, help to develop surgical procedures and to guide the operation of lymphatic dissection, effectively shorten the operation time, avoid damage to blood vessels, reduce the amount of intraoperative blood loss, and achieve a truly minimally invasive purposes.
    Expression and significance of Wnt/βcatenin signal transduction pathway in papillary thyroid carcinoma
    YAN Li, LI Qinghuai, JI Hong, HAO Fang
    2017, 37(6):  663-667.  doi:10.3969/j.issn.10073205.2017.06.011
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    Abstract］  Objective To study expression and significance of Wnt inhibitory factor1(WIF1), βcatenin and chclin D1 in Wnt/βcatenin signal transduction pathway in papillary thyroid cancer（PTC）. Methods Sixty cases of PTC were selected and the specimens of cancerous foci and adjacent tissues were taken. The expression of WIF1, βcatenin and cyclin D1 mRNA and protein were detected, and the correlation between WIF1 mRNA and βcatenin, cyclin D1 mRNA was analyzed. ResultsThe expression levels of WIF1 mRNA and positive rates of WIF1 protein expression in PTC tissues were significantly lower than those in paracancerous tissues(P＜005), while expression levels of βcatenin, cyclin D1 mRNA and protein in PTC tissues were on the contrary(P＜005). And the expression levels of WIF1 mRNA and protein in PTC lymph node metastasis group was lower than those without lymph node metastasis, but the expression levels of βcatenin, cyclin D1 mRNA and protein in PTC lymph node metastasis group were on the contrary(P＜005). There were no statistically significant differences in the expression levels of WIF1, βcatenin, cyclin D1 mRNA and positive rates of protein in PTC tissues in different sex, age, tumor diameter, number of lesions and whether or not the capsule was invaded(P＞005). Expression levels of βcatenin, cyclin D1 mRNA were negatively correlated with those of WIF1 mRNA(P＜005). ConclusionThe down regulation mechanism of WIF1 gene in PTC tissues promoted the accumulation of βcatenin protein in the cytoplasm, which resulted in over activation of target gene cyclin D1. The WIF1 down regulation or βcatenin and cyclin D1 up regulation mechanism may play an auxoaction role in the occurrence and metastasis in PTC.
    Effect of continuous negative pressure drainage in the postoperative #br# recovery of tuberculous empyema necessitates
    LIU Jiakun, ZHAO Dawei, CHEN Lihui, WANG Mingzheng*, QIE Donglei, HAN Qingsong
    2017, 37(6):  668-671.  doi:10.3969/j.issn.10073205.2017.06.012
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    Abstract］ ObjectiveTo explore the effect of continuous negative pressure drainage in the postoperative recovery of tuberculous empyema necessitatis. MethodsSixtyeight patients with tuberculous empyema necessitatis were researched, 34 patients treated by continuous negative pressure drainage were in the observation group,and the other 34 patients treated by passive drainage were in the control group. The amount of postoperation drainage, drainage duration, postoperative visual analogue scale(VAS) score, postoperative hospital stay, lung recruitment, and the rate of poor incision healing were compared between the two groups. ResultsThe drainage duration and the postoperative hospital stay of the observation group was shorter than the control group, the difference was statistically significant(P＜005). The amount of postoperation drainage and the postoperative VAS score of the two groups were not significantly different(P＞0.05). The rate of poor incision healing of the observation group was lower than the control group, the difference was statistically significant(P＜005). ConclusionOn the basis of strict indications, continuous negative pressure drainage showed many positive effects in postoperative recovery of tuberculous empyema necessitates.
    Clinical study of Qswitched 1 064 nm laser combined with Xiaoyao Pill in the treatment of chloasma
    HAN Lanqin, ZHANG Chunli, ZHAO Xuelian, ZHANG Jie, HAN Peng， LUO Xinshu
    2017, 37(6):  672-675.  doi:10.3969/j.issn.10073205.2017.06.013
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    ［Abstract］  ObjectiveTo discuss the clinical efficacy and safely of the Qswitched 1 064 nm laser combined with  free and Xiaoyao Pill in the treatment of chloasma. MethodsForty cases patients with chloasma were randomly divided into two groups. The treatment group(20 cases) was given Qswitched 1 064 nm laser combined with free and Xiaoyao Pill treatment. The control group(20 cases) was given a simple Qswitched 1 064 nm laser treatment. The skin melanin/erythema/wrinkle index were measured with a skin evaluation instrument before and after each treatment. ResultsAfter  treatment， 2 groups of  melanin index, erythema index, wrinkle index decreased gradually. The treatment group was lower than the control group. There was significant difference in interaction between different groups, different time points and between different time points of groups(P＜005). ConclusionThe Qswitched 1 064 nm laser combined with free and Xiaoyao Pill on chloasma is a safe and effective and not easy to relapse.
    Differences in IL10 and IL12 expression of different tumorassociated macrophage subtypes in ascites of ovarian carcinoma
    SONG Yuxia, ZHAO Tao, WANG Hongwei, GUO Qing*, WANG Xuejuan
    2017, 37(6):  676-679.  doi:10.3969/j.issn.10073205.2017.06.014
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    [Abstract］ ObjectiveTo detecte M2/M1 ratio of ascites or peritoneal washings of different macrophages subtypes of ovarian cancer patients at different stages, to separate M1 subtype macrophages and M2 subtype macrophages by flow cytometry, to test the mRNA expression of M1 and M2related cytokines IL10 and 1L12, and to explain the immune mechanism of ovarian cancer abdominal metastasis at the point of cytokines. Methods①M1 macrophages and type M2 macrophages were labeled with CD64 and CD163 respectively, and the ratio of M2/M1 in ascites or peritoneal lavage fluid in 10 cases of ovarian cancer and 17 cases of benign ovarian tumor was observed. ②Flow cytometry was used to separate M1 subtype macrophages and M2 subtype macrophages. ③RTPCR was used to test the mRNA expression of M1 and M2related cytokines IL10 and 1L12. ResultsM2/M1 ratio in control group was lower than that in Ⅰ+Ⅱ stage and  Ⅲ+Ⅳ stage; while Ⅰ+Ⅱ stage M2/M1 ratio was lower than  Ⅲ+Ⅳ stage, and there was statistically significant differen
    Expression and significance of TLR4, MyD 88 and NFκB p65 in cholesterol pneumonia
    KANG LIfei1, DI Hongqin2, ZHU Guiyun1，AN Xiaoying1, CHEN Ning1, YANG Yonghui1*
    2017, 37(6):  680-684.  doi:10.3969/j.issn.10073205.2017.06.015
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    Abstract］  ObjectiveTo detect the expression of Tolllike receptor 4（TLR4）, Myeloid differentiation factor 88（MyD88）and nuclear factorκB p65（NFκB p65） in cholesterol pneumonia foam cells, and to investigate their roles and relationship in the occurrence of cholesterol pneumonia and clinical significance. MethodsThe expression of TLR4, MyD88 and NFκB p65 in 45 cases of foam cells of cholesterol pneumonia and 25 cases of macrophages of usual pneumonia without the aggregation of foam cells(control groups) from surgically resecting specimens of lung that was confirmed by pathology was immunohistochemically studied. ResultsThe strong positive rate of MyD88 and NFκB p65, as well as the positive rate of TLR4, was obviously higher than that in the control(P＜005) The positive expression of TLR4,MyD88 and NFκB p65 was not related with sex(P＞005).The expression rate of TLR4,MyD88 and NFκB p65 in patience below 50 was more significant higher than the patience above 50(P＜005). Among the 45 cases of cholesterol pneumonia , there were apparently positive correlation between TLR4 and MyD88, TLR4 and NFκB, NFκB p65 and MyD88(r=0.467, 0.523,  0.491, P＜005),as well as the positive correlation. ConclusionThe expression of TLR4, MyD88 and NFκB p65 may be involved in pathogenesis of cholesterol pneumonia presenting their synergistic effect in cholesterol pneumonia, which was not related to the sex but age. With the increase in age of the cholesterol pneumonia, the positive rate of TLR4, MyD88 and NFκB p65 was decreased.
    The effects of increasing occlusal vertical dimension on mRNA of myosin heavy chain and myogenic regulatory factors in the deep masseter of rat at different ages
    WANG Wen1, LIU Chunyan1, LU Haiyan1, MA Wensheng1, HU Xiaoying1, DONG Fusheng2*
    2017, 37(6):  685-690.  doi:10.3969/j.issn.10073205.2017.06.016
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    ［Abstract］ ObjectiveTo investigate the influence of increasing occlusal vertical dimension(iOVD) on mRNA of myosin heavy chain and myogenic regulatory factors in deep masseter of rat at different ages. MethodsFortyeight male Wistar rats were divided into two groups: teenage group(1.5 months, n=24) and young adult group(8 months, n=24). Then the two groups were randomly divided into control and experimental group(n=12). The experimental groups were iOVD by placing composite resin on molars. So there were four groups according to the age and experimental intervention, namely teenager control group, teenager experimental group, young adult control group and young adult experimental group. The mRNA of the MHC, MyoD and myogenin of deep masseter were observed on day 7 and day 14 after iOVD. ResultsOn the 7th day of iOVD, the relative level of MHCⅡa mRNA in group TE was higher than that in group TC. Aslo MHCⅡa mRNA levels in group YAC and group YAE were lower than that in group TC and TE（P＜005）. On the 14th day of iOVD, the relative level of MHCⅡa mRNA in group TE and YAE was higher than that in group TC and YAC. MHCⅡa mRNA levels in group YAC and group YAE were lower than that in group TC and TE（P＜005）. The relative level of MHCⅡx mRNA in group TE and group YAE was lower than that in group TC and group YAC on the 7th and the 14th day of iOVD（P＜005）. On the 7th day of iOVD, the relative level of MHCⅡb mRNA in group TE was higher than that in group TC. Aslo MHCⅡb mRNA level in group YAC and group YAE were higher than that in group TC and TE（P＜005）. On the 14th day of iOVD, the relative level of MHCⅡb mRNA in group TE and YAE was higher than that in group TC and YAC. Aslo MHCⅡb mRNA level in group YAC and group YAE were lower than that in group TC and TE（P＜005）. On the 7th and the 14th day of iOVD, the relative level of MyoD mRNA in group TE and group YAE was higher than that in group TC and YAC. The relative level of MyoD mRNA in group TE and YAE was higher than that in group TC and YAC(P＜005). The relative level of myogenin mRNA and myogenin:MyoD mRNA in group TE and group YAE was higher than that in group TC and group YAC, group YAC and group YAE was lower than group TC and group TE on the 7th and the 14th day of iOVD（P＜005）.There was positive correlation between the myogenin/MyoD mRNA ratio and MHC Ⅱa mRNA(r=0.954， P＜0.05), and negative correlation between myogenin/MyoD mRNA ratio and MHC Ⅱb mRNA(r=-0.755， P＜0.05). ConclusionThe deep masseter of teenager rat in experimental group changes to MHC Ⅱa mRNA earlier and more than that of young adult rat. The MyoD and myogenin which belong to myogenic regulatory factors plays an important role in fiber reconstruction.
    Clinical application of 3D printed implant guide in immediate implantation of posterior teeth
    ZHAO Xiaojun, WANG Huichao, XU Yunhai, HU Yongquan, LI Chuang
    2017, 37(6):  691-694.  doi:10.3969/j.issn.10073205.2017.06.017
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    ［Abstract］  ObjectiveTo evaluate the clinical effect and accuracy of 3D printing implant template applied to the immediate implantation in posterior teeth areas. MethodsTwenty patients of the immediate implantation in posterior teeth are selected. After cone beam computed tomography（CBCT） scan of oral maxillofacial, silicone rubber impression, perfusion model, the registration is required between CBCT image and plaster model scanning data in order to compound a three dimensional jaw model. The implant position is designed via Simplant software and then 3D implant template is ready to fabricate. Under guide of template, the implant socket is prepared for implants and CBCT is required after the operation.  Through Mimics software, the differences between designing position and actual position of implant should be measured mainly in neckhorizontal deviation distance, apical deviation distance as well as axial deviation angle. ResultsThe three dimensional position of twenty implants respectively in twenty patients are basically as same as the designing position with neckhorizontal deviation distance of(0.65±0.19) mm, apical deviation distance of(0.45±0.14) mm, axial deviation angle of(3.20±0.68) °. For 6-12 months of followup after restoration, no implant loosens off, the stability of bones around implant is sound without distinct bone resorption. The implant coping restoration can offer a good occlusion. Conclusion3D printing surgical guide can accurately transfer designing plan into the operation, improve precision of implantation and reduce the operation risk and complications. Also, it is beneficial to restore upper copings in later period and plays an important role in the difficult immediate implantation of posterior teeth.
    The shortterm curative effects of endoscopic sinus surgery influenced by different duration of oral glucocorticoidtreatment in patients with chronic rhinosinusitis and nasal polyps
    SUN Hengya1, LIU Tao2
    2017, 37(6):  695-698,704.  doi:10.3969/j.issn.10073205.2017.06.018
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    Abstract］ ObjectiveTo observe the effect of perioperative corticosteroid on the shortterm efficacy and adverse reactions in patients with chronic rhinosinusitis and nasal polyps. MethodsOne hundred and fifty chronic rhinosinusitis with nasal polyps patients were randomly divided into 3 groups, each group with 50 patients. In the perioperative period, the 3 groups were respectively taken none oral glucocorticoids, 2 weeks oral glucocorticoids, and 4 weeks oral glucocorticoids therapy. We evaluate the efficacy of recent treatment, compare the occurrence of intraoperative bleeding and complications, and assess the incidence of systemic adverse reactions with oral corticosteroids. ResultsTotal effective rate of group A was 78.0%. Total effective rate of group B was 94.0%. Total effective rate of group C was 92.0%. Group B and group C were both more efficient than group A(P＜0.05), and the difference between group B and group C was not statistically significant(P＞005). The amount of bleeding in group B and group C were less than that in group C, and the difference between the two groups was statistically significant. Postoperative bleeding>50mL in group B and group C were less than group A(P＜005), there was no significant difference in the number of patients with orbital congestion in the 3 groups（P＞005）. ConclusionPerioperative oral glucocorticoids have obvious promoting effect for endosopic sinus surgery treatment in patients with chronic rhinosinusitis and nasal polyps. There was no statistically significant difference between 2 weeks of oral corticosteroids and 4 weeks. To reduce the incidence of adverse events and to reduce the cost of care, perioperative corticosteroids are recommended for 2 weeks.
    Performance evaluation of Beckman Coulter DxH800 hematology analyzer
    YANG Hongle, YANG Limiao*, WANG Ran, LIU Xueguang, DU Huanhuan, SONG Jianqiang
    2017, 37(6):  699-704.  doi:10.3969/j.issn.10073205.2017.06.019
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    ［Abstract］ ObjectiveTo verify and evaluate the main performance system of Beckman Coulter DxH800 Hematology Analyzer. MethodsRefer to industry standard of WS/T 4062012 analytical quality specifications for routine tests in clinical hematology and Instrument specification，the background count, carryover rate, precision, linearity range, reportable range, accuracy, comparability of results in laboratory, degree of preciseness of Beckman Coulter DxH800 hematology analyzer were verified. ResultsThe background count, carryover rate, interbatch precision and inter day precision of Beckman Coulter DxH800 Hematology Analyzer can all satisfy the requirements of Instrument specification and WS/T 4062012. Linear verification of white blood cell, red blood cell, hemoglobin and platelet can all satisfy the requirements of a value in the 1±0.05 range and the correlation coefficient (r)＞0.975. The results of clinical specimens which covered the medical decision level range had a good linear relationship with the dilution ratio(r>0.990). That the reported range of measurement was larger than that of the instrument, which can meet the clinical needs. The bias of the mean and the constant value of samples of the accuracy verification can content the requirements. It had a good correlation between Beckman Coulter DxH800 and Beckman Coulter LH750 Hematology Analyzer. The relative deviations of the items in the accuracy verification which accorded the requirements were obviously lower than the required values. ConclusionThe performance indexes of Beckman Coulter DxH800 Hematology Analyzer showed excellent performance，and it could meet the requirement of the analysis quality, and could meet the clinical work well.
    Analysis of the application of narcotic analgesic drugs in Chongqing cancer hospital during 2013-2015
    LIU Jing, CHEN Kaijie, JIA Yimin
    2017, 37(6):  705-709.  doi:10.3969/j.issn.10073205.2017.06.020
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    ［Abstract］ ObjectiveTo investigate the use of narcotic analgesic drugs in outpatient and inpatient department in Chongqing cancer hospital, and to provide reference for rational use of drugs by exploring the structure and trend of clinical medication in a hospital. MethodsThe types and numbers of the use of narcotic analgesic drugs were collected from a hospital during 2013-2015, and then taking defined daily dose（DDD） as objective indicators, analyzed retrospectively in respects of consumption sum, use frequency and DDC, etc. ResultsFor three years, the sort of narcotic analgesic drugs in a hospital remained stable, total consumption sum increased year by year, and the form of drugs and dosage of oral sustained release preparation increased constantly. The outpatient department is mainly oral morphine preparation and oxycodone sustained release preparation. The defined daily dose system(DDDs) of morphine hydrochloride sustained release tablets(30 mg) ranked the top, and the DDDs of pethidine injection and hydromorphone injection which was a new variety ranked the bottomand and the inpatient department is mainly fentanyl preparation and morphine sustained release preparation. ConclusionThe varieties and dosage forms of narcotic analgesics in the hospital are more suitable for clinical use. Strictly follow the principle of oral administration, the third ladder drugs represented by morphine are the first choice for patients with cancer pain.