Table of Content

25 October 2017, Volume 38 Issue 10

Previous Issue    Next Issue

p38 MAPK signaling pathway participates in the sulbactaminduced brain ischemic tolerance in rats

XIAN Xiaohui, GAO Junxia, QI Jie, LI Wenbin

2017, 37(10):  1117-1121，1126.  doi:10.3969/j.issn.10073205.2017.10.001

Asbtract ( 911 )   PDF (1192KB) ( 118 )  

Related Articles | Metrics

［Abstract］ Objective〖HTSS〗〓To investigate the role of p38 MAPK signaling pathway in sulbactaminduced brain ischemic tolerance in rats.
〖HTH〗〖WTHZ〗Methods〖HTSS〗〓The model of rat global cerebral ischemia was used. After a stainless steel cannula was implanted in the right lateral ventricle，25 healthy male Wistar rats were randomly divided into the following groups(n=5 in each group). ①Sham group（normal saline+sham）: the rats were administrated with normal saline of 10 μL via the cannula first, and then the sham operation for global cerebral ischemia was performed. ②Global cerebral ischemia group（ischemia）: normal saline(10 μL) was administrated via the cannula first and then performed 8 minutes of global cerebral ischemia immediately. ③Sulbactam+ischemic group: sulbactam solution(10 μL, 360 nmol) was administrated via the cannula first and then performed 8 minutes of global cerebral ischemia immediately. ④SB203580+sulbactam+ischemic group: SB203580(10 μL, 5 nmol) was administrated via the cannula 30 minutes before sulbactam(10 μL, 360 nmol) and then performed 8 minutes of global cerebral ischemia immediately. ⑤SB203580+sham group: SB203580 solution(10 μL, 5 nmol) was administrated via the cannula first and then performed sham operation for global cerebral ischemia immediately. The neuropathological evaluation including histological grade(HG) and neuronal density(ND) was performed using the method of thionic staining to observe the survival situation of neurons in CA1 hippocampus.
〖HTH〗〖WTHZ〗Results〖HTSS〗〓Global cerebral ischemia for 8 min induced obvious delayed neuronal death（DND）which resulted in large area of neuronal absence in the CA1 hippocampus with the significant increase in HG and decrease in ND. Pretreatment with sulbactam effectively prevented the DND normally induced by the global brain ischemia. Compared with ischemic group，the HG was significantly reduced, and the ND increased significantly. Pretreatment with SB203580, an inhibitor of p38 MAPK signal pathway, blocked the neuroprotective effect mediated by sulbactam. Compared with sulbactam+ischemic group, the ND was significantly decreased, and the HG increased significantly.
〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓p38 MAPK signaling pathway may participate in the sulbactaminduced brain ischemic tolerance in rats.


Study of the relationship between bloodlipid ratio and TOAST classification & intracranial vascular stenosis#br#

GU Lili1,2, TIAN Yongwu2, HE Dan1*

2017, 37(10):  1122-1126.  doi:10.3969/j.issn.10073205.2017.10.002

Asbtract ( 539 )   PDF (403KB) ( 91 )  

Related Articles | Metrics

［Abstract］ Objective〖HTSS〗〓To explore the relationship between the levels of individual blood lipid/lipid ratio and the types of cerebral infarction as well as the severity of intracranial vascular stenosis in patients with acute cerebral infarction.
〖HTH〗〖WTHZ〗Methods〖HTSS〗〓 Two hundred and nineteen patients were included in the study. According to TOAST classification, all patients were divided into three  groups,  including 95  patients with large atherosclerotic(LAA), 79 patients with small arterial occlusive(SAO) and 45 patients with cardiogenicembolism(CE). According to the degree of infarction, 73 cases were moderate stenosis, 57 cases were severe stenosis, 89 cases were complete occlusion. The total cholesterol(TC), triglyceride(TG), low density lipoprotein cholesterol(LDLC) and high density lipoprotein cholesterol(HDLC) levels were measured and the ratios of TC/HDLC, TG/HDLC, and LDLC/HDLC were calculated.
〖HTH〗〖WTHZ〗Results〖HTSS〗〓 The ratio of  TC/HDLC in LAA type of patients was significant higher than that in CE and SAO patients, and it was also higher in CE than in  SAO  patients(P＜005). The ratio of TG/HDLC in CE patients was significant lower than that in SAO(P＜005). The ratio of serum TC/HDLC was the highest in the severe stenosis group, and it was significant lower in the moderate stenosis patients than that in the severe stenosis patients(P＜005).
〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓In the TOAST subtype, the serum TG/HDLC ratio can be used as a predictor of etiologic classification in the early onset of cerebral infarction in SAO type of patients. The TC/HDLC ratio is expected to be a specific biomarker of LAA, especially for early prediction of LAA. TC/HDLC ratio has an important reference value in judging severity of intracranial vascular stenosis.


Protective effect of M type potassium channel opener in myocardium of cardiopulmonary resuscitation rats#br#

WANG Ruigang1, ZHAO Chunxiang1, LI Yuelan2, QI Haitao2, ZHANG Lei3, SHI Qiuyan1

2017, 37(10):  1127-1131.  doi:10.3969/j.issn.10073205.2017.10.003

Asbtract ( 884 )   PDF (398KB) ( 89 )  

Related Articles | Metrics

［Abstract］ Objective〖HTSS〗〓To explore the protective effect of Mtype potassium channel opening agent on myocardium of cardiopulmonary resuscitation rats.
〖HTH〗〖WTHZ〗Methods〖HTSS〗〓A total of 60 healthy Wistar rats were randomly divided into sham operation group(group A), cardiac resuscitation group(group B), Mtype potassium channel opener intervention group(group C), and there were 20 rats in each group. Four rats were sacrificed in each group at 6 h, 12 h, 24 h, 48 h and 72 h after cardiopulmonary resuscitation recovery. The myocardium samples were collected, the myocardial parameters(superoxide dismutase, malondialdehyde, cardiac troponin T) were detected, and the cardiac structure(left ventricular volume, ventricular septal thickness) were observed.
〖HTH〗〖WTHZ〗Results〖HTSS〗〓Compared with group A, the levels of superoxide dismutase at any time point in group B were all lower, and those in group C were higher than group B , the difference were significant(P＜005). Both in group B and in group C, the levels of superoxide dismutase at 12 h and 24 h were all decreased gradually, but were increased at 48 h and 72 h, and the difference were significant(P＜005). The levels of malondialdehyde , cardiac troponin T, left ventricular volume, and ventricular tachycardia at any time point in group B were higher than those in group A, and those in group C were lower than group B. The ventricular septal thickness of group C was thicker than that of group A 24 h after operation. Both in group B and in group C, the levels of malondialdehyde  and cardiac troponin T at 12 h and 24 h were increased gradually, but were decreased at 48 h and 72 h. And the left ventricular volume were the same changes in group C. The difference were significant(P＜005).
〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓Mtype potassium channel opener can protect myocardial cells and reduce the impairment on myocardial of cardiopulmonary resuscitation rats.


Effective observation of insulin determir combined with acarbose in treatment of 34 cases of type 2 diabetes mellitus

YANG Liu, TIAN Li, MENG Xianjie, LIU Hongmei, ZHANG Jinji

2017, 37(10):  1132-1134,1140.  doi:10.3969/j.issn.10073205.2017.10.004

Asbtract ( 702 )   PDF (373KB) ( 139 )  

Related Articles | Metrics

［Abstract］  Objective〖HTSS〗〓To observe the efficacy and safety of initiating insulin therapy with insulin detemir in type 2 diabetes mellitus(T2DM).
〖HTH〗〖WTHZ〗Methods〖HTSS〗〓Sixtyeight subjects with T2DM were randomly divided into two groups. Fasting blood glucose, postprandial 2 h blood glucose, glycosylated hemoglobin, fasting C peptide, postprandial 2 h C peptide, daily insulin dosage, body mass index, and hypoglycemic events were compared between the 2 groups before and after treatment.
〖HTH〗〖WTHZ〗Results〖HTSS〗〓After treatment, fasting blood glucose, postprandial 2 h blood glucose and glycosylated hemoglobin levels of the 2 groups were significantly lower than those before treatment. The level of 2 h C peptide in the treatment group was significantly higher than that before the treatment, the difference was statistically significant(P＜005). After treatment, the incidence of hypoglycemia in the treatment group was significantly lower than that in the control group, and the body mass index of the treatment group was significantly lower than that of the control group, the difference was statistically significant（P＜005）. Fasting blood glucose, postprandial 2 h blood glucose, glycosylated hemoglobin, fasting C peptide, postprandial 2 h C peptide level and daily insulin dosage at the end of the 2 groups were not statistically significant（P＞005）.
〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓Insulin detemir combined with acarbose hypoglycemic will not increase the body weight and the incidence of hypoglycemia, and can promote the repair of islet function.


Expression and prognostic significance of TAMs and Ki67 in non small cell lung cancer

WEI Yusen1， YANG Longfei1， ZHANG Xiaolu1， ZHANG Hongzhen1，2*

2017, 37(10):  1135-1140.  doi:10.3969/j.issn.10073205.2017.10.005

Asbtract ( 846 )   PDF (1431KB) ( 105 )  

Related Articles | Metrics

［Abstract］ Objective〖HTSS〗〓To investigate the expression levels of tumor associated macrophages(TAMs), tumor proliferation factor(Ki67) in nonsmall cell lung cancer(NSCLC) and their relationships with the clinical pathological features, and to study its relationship with prognosis.
〖HTH〗〖WTHZ〗Methods〖HTSS〗〓Immunohistochemical SP method was used to analyze the expressions of CD68(TAMs surface marker) Ki67 and in 68 cases of NSCLC tissues and 17 cases of normal lung tissues. According to the results of followup, KaplanMeier survival analysis and Cox regression analysis of CD68, Ki67 was used to study its relationship with lung cancer prognosis.
〖HTH〗〖WTHZ〗Results〖HTSS〗〓The positive rates of CD68 and Ki67 in 68 cases of lung cancer were 66.2%(45/68) and 61.8%(42/68). The positive expression rates of CD68 and Ki67 in normal lung tissues were 17.6%(3/17) , 5.9%(1/17), the difference was statistically significant(P＜005). The expression of CD68 and Ki67 in NSCLC tissues with high TNM stage, lymph node metastasis and invasion were significantly increased(P＜005).In addition, the expression of Ki67 in poorly differentiated patients increased. There was a positive correlation between CD68 and Ki67 expression in NSCLC(r=0.461, P=0000). Sixtyeight patients with NSCLC 1, 3, 5year survival rate 〖JP2〗were 86.76%, 64.71%, 16.18%. Cox multivariate analysis showed that the low degree of differentiation〖JP〗 and lymph node metastasis are risky factors for adverse prognosis(P＜005).
〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓CD68, Ki67positive lung cancer patients with poor prognosis. CD68 and Ki67 play a role in the development, invasion and metastasis of NSCLC, and combined detection of them provide a potential target for clinical diagnosis, adjuvant therapy of NSCLC.


Epidemiological analysis of jellyfish stings in 2 577 cases in Qinhuangdao#br#

HUO Shuhua1, TIAN Yingping1, ZHANG Yukun1, SU Xiaoyun1, LIU Jiqiang2

2017, 37(10):  1141-1143,1157.  doi:10.3969/j.issn.10073205.2017.10.006

Asbtract ( 1020 )   PDF (424KB) ( 235 )  

Related Articles | Metrics

［Abstract］ Objective〖HTSS〗〓To analyze the epidemiological data and clinical features of jellyfish stings, and to provide scientific basis for prophylaxes,  diagnosis and treatment.
〖HTH〗〖WTHZ〗Methods〖HTSS〗〓The 2 577 clinical cases of jellyfish stings in Beidaihe hospital of Qinhuangdao were restrospectively analyzed. The epidemiological characteristics, clinical symptom, diagnosis, treatment method and prognosis were included in the descriptive statistical analysis.
〖HTH〗〖WTHZ〗Results〖HTSS〗〓Of the 2 577 cases, there were 1 313 children, 1 264 adults, aged from 0.5 to 78 years(male 1 508, female 1 069).  Lower limbs were most frequently stinged, account for 70.94%. The majority of jellyfish stings were mild in 2 536 patients(98.41%), 33 in moderate patients(1.28%), and 8 in severe patients(0.31%), with no deaths. In recent 3 years, the number of jellyfish stings cases has been decreasing year by year.
〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓Jellyfish stings should be based on prevention, and effective preventive measures can significantly reduce the incidence. The treatment should be foccured on the anaphylactic reaction and local processing in therapy and preventing  the complication in order to decrease fatality rate.


Effects of adriamycin combined with thermotherapy and immunotherapy on glioma cells#br#

ZHANG Haifeng1， ZHENG Shiya2， OUYANG Bin1, YANG Dengyuan1, LIU Wenqing3， TIAN Jihui3*

2017, 37(10):  1144-1147.  doi:10.3969/j.issn.10073205.2017.10.007

Asbtract ( 535 )   PDF (377KB) ( 113 )  

Related Articles | Metrics

［Abstract］ Objective〖HTSS〗〓To observe the effect of adriamycin combined with thermotherapy and antiprogrammed cell death ligand1(PDL1) antibodies on cell proliferation in U251 glioma cells, and to explore the expression of PDL1 of U251 glioma cells after being treated with adriamycin and/or thermotherapy.
〖HTH〗〖WTHZ〗Methods〖HTSS〗〓IC50 of adriamycin for U251 cells was determined by CCK8 assay. The U251 cells were divided into several groups according to different treatments, then the viability of cells were measured by CCK8 assay. The expression of PDL1 on the surface of U251 cells was determined by flow cytometry after treated with adriamycin and/or thermotherapy.
〖HTH〗〖WTHZ〗Results〖HTSS〗〓Adriamycin combined with thermotherapy and antiPDL1 antibodies could inhibit glioma cells growth obviously compared with other groups. Adriamycin combined with thermotherapy could upregulate the expression of PDL1.
〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓Adriamycin combined with thermotherapy and antiPDL1 antibodies is a promising treatment regime for glioma and our research provides theoretical basis for the combination of adriamycin, thermotherapy and immunotherapy in glioma treatment.

The application value of thrombelastograghy in evaluating deep vein thrombosis after severe traumatic brain injury#br#

ZHANG Liqiao

2017, 37(10):  1148-1152,1167.  doi:10.3969/j.issn.10073205.2017.10.008

Asbtract ( 466 )   PDF (427KB) ( 112 )  

Related Articles | Metrics

［Abstract］ Objective〖HTSS〗〓To investigate the application value of thrombelastography(TEG) in the dynamic monitoring deep vein thrombosis among patients with severe traumatic brain injury(sTBI).
〖HTH〗〖WTHZ〗Methods〖HTSS〗〓One hundred and sixteen cases of patients with sTBI were randomly divided into 2 groups：experimental group(65 cases) and control group(51 cases). The prothrombin time(PT), activated partial thromboplastin time(APTT), thrombin time（TT）, fibrinogen(FIB), blood platelet(PLT) and thrombus elastic figure index （R, K, Angle and MA）were observed between the two groups in preoperation and 1, 3, 7, 14 days postoperative. TEG tests were performed in preoperation on experimental group patients, and given low molecular weight heparin calium treatment according to the result of measuring, and continue to TEG monitoring on 1, 3, 7, 14 days in postoperation. The control group patients were performed the traditional experiential therapy after operation according to coagulation and platelet results. Both groups of patients were separately recorded the occurrence of deep vein thrombosis by the color doppler ultrasound of lower limbs on 2 weeks.
〖HTH〗〖WTHZ〗Results〖HTSS〗〓TT and APTT value were gradually rise in the two groups and there was statistical difference between the different points（P＜005）. PT value fluctuated in 2 groups and there was statistical difference between the different points（P＜005）. FIB value was downward trend in the two groups and there was statistical difference between groups, the different points and the interaction（P＜005）. PLT value was declined in the two groups and there was statistical difference between the different points（P＜005）. The incidence of DVT was 7.69%(5/65) on experimental group, the incidence of DVT was 25.49%(13/51) on control group, the differences were statistically significant(χ2=6.905， P＜005).
〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓TEG has a higher application value in monitoring deep vein thrombosis after severe traumatic brain injury compared with conventional coagulation routine test.


Clinical application of spiral blade insert assisted locator for proximal femoral nail antirotation#br#

PU Xiaopeng1, XU Ke1, REN Qiang1, XING Lei2, LI Xicheng1*

2017, 37(10):  1153-1157.  doi:10.3969/j.issn.10073205.2017.10.009

Asbtract ( 499 )   PDF (842KB) ( 103 )  

Related Articles | Metrics

［Abstract］ Objective〖HTSS〗〓To analyze the accuracy of auxiliary positioner of proximal femoral nail antirotation（PFNA）in the clinical application.
〖HTH〗〖WTHZ〗Methods〖HTSS〗〓Selected 40 patients with intertrochanteric fracture of the femur, according to the doubleblind method, were divided into control group and observation group. The first 20 patients in the control group were received conventional surgery by PFNA. The rest 20 patients in the observation group were received PFNA surgery with screwblade implanted auxiliary positioner. The number of implantation, the duration of operation and the number of Carm Xray exposure were recorded. The accuracy of the placement was evaluated by measuring the angle between the axis of the screw blade and the central axis of the femoral head(deflection angle).
〖HTH〗〖WTHZ〗Results〖HTSS〗〓The operation time, Xray exposure time, the number of screw inserts and the deflection angle of the observation group were less than those of the control group. The difference was statistically significant(P＜0.05).
〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓The proximal femoral screw counter screw blade assisted positioner allows for faster and more accurate placement of the screw blade at once. It can obviously reduce the exposure times of Carm and effectively reduce the radiation damage of related personnel.


Analysis of therapeutic effect of modular decompression combined with internal fixation for treatment of lumbar disc herniation#br#

WANG Rui, HUO Xiwei, HU Chengdong, LI Dongfeng, LIU Fajing, WANG Fei

2017, 37(10):  1158-1162.  doi:10.3969/j.issn.10073205.2017.10.010

Asbtract ( 458 )   PDF (1797KB) ( 139 )  

Related Articles | Metrics

［Abstract］  Objective〖HTSS〗〓To observe the clinical effect of modular decompression combined with interbody fusion in the treatment of lumbar disc herniation.
〖HTH〗〖WTHZ〗Methods〖HTSS〗〓Seventyfive cases of lumbar disk hernitaion patients were divided into 2 groups based on the operation procedure they accepted. Thirtysix patients accepted modular decompression combined with interbody fusion(modular decompression group). Thirtynine patients accepted normal laminectomy combined with interbody fusion(conventional decompression group). The operative time, intraoperative blood loss, Japanese Orthopaedic Association(JOA) scores and Visual Analogue Scale(VAS) were recorded and analyzed.
〖HTH〗〖WTHZ〗Results〖HTSS〗〓No spinal cord and nerves injury occurred during the surgery. The operation time and intraoperative blood loss in modular decompression group were lower than those in conventional decompression group(P＜005). The JOA scores of the 2 groups were gradually increased when compared with the preoperation(P＜005). There was no significant difference between groups and intergroup interaction between time points(P＞005). The VAS score of lower back pain in the 2 groups were first increased and then decreased(P＜005), and modular decompression group was lower than conventional decompression group(P＜005). There was significant difference among groups, time points and intergroup interaction between time points(P＜005).
〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓Compared with the normal laminectomy, modular decompression could reduce the operation time, control the intranopative blood loss and decrease the low back pain, which is an effective decompression method for the treatment of lumbar disc herniation.


Effect of quality control circle in improving the accuracy of the risk assessment of pressure sores in hospitalized patients in different levels of orthopedic nurses

LIU Huiling1， WANG Panpan2， DONG Hongwei2， WANG Weijie3， LI Na2， CHEN Xiaoli2

2017, 37(10):  1163-1167.  doi:10.3969/j.issn.10073205.2017.10.011

Asbtract ( 552 )   PDF (421KB) ( 148 )  

Related Articles | Metrics

［Abstract］ Objective〖HTSS〗〓To explore the effect of quality control circle （QCC） in improving the accuracy of the risk assessment of pressure sores in hospitalized patients in different levels of orthopedic nurses.
〖HTH〗〖WTHZ〗Methods〖HTSS〗〓We randomly selected 112 cases respectively from before the intervention of the QCC(ApriMay 2014) and after the intervention(AugustSeptember 2014) from orthopedic hospitalized patients,who's Braden pressure sores risk score≤16 points. According to the steps of quality control circle, the reasons for low risk assessment of pressure sore in department of orthopedics nurses were analyzed, and the countermeasures were put forward and implemented according to the real cause.
〖HTH〗〖WTHZ〗Results〖HTSS〗〓The prognosis of the deficit project was significantly less than that before the intervention, and the rate of the two items of friction / shear and sensation after intervention was lower than that before the intervention. The evaluation accuracy of nurses at all levels was significantly higher than before intervention, and the average scores of theoretical knowledge of pressure ulcer test were higher than those before intervention, and the differences were statistically significant（P＜0.01）. The incidence of pressure sores in phase I decreased to 2 cases(1.79%) after the intervention,compared with 9 cases(8.04%) before the intervention,there also have statistical significance（P＜0.01）.
〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓The quality control circle activities improved the accuracy of the assessment of the risk of pressure sore in patients with department of orthopedics, and effectively improved the awareness of nurses and patients to prevent pressure ulcers, and reduced the occurrence of pressure sores.


Effect of FGF+Collagen combined with mupirocin ointment in treating residual burn wounds on the expression of inflammatory factors and wound healing#br#

XU Lihong

2017, 37(10):  1168-1171.  doi:10.3969/j.issn.10073205.2017.10.012

Asbtract ( 504 )   PDF (380KB) ( 249 )  

Related Articles | Metrics

［Abstract］ Objective〖HTSS〗〓To explore the impact of fibroblast growth factor(FGF）+Collagen combined with Bactroban on the expression of the inflammatory factors and wound healing in the treatment of residual burn wounds.
〖HTH〗〖WTHZ〗Methods〖HTSS〗〓Eighty patients were randomly divided into the control group（n=40） and the observation group（n=40）. Bactroban was applied in the control group, while FGF+Collagen biologic protein sponge combined with Bactroban were applied in the observation group. The clinical efficacy, wound repair, the expression of the inflammatory factors and the quality of wound healing of the two groups were compared.
〖HTH〗〖WTHZ〗Results〖HTSS〗〓The total effective rate of the observation group was obviously higher than that of the control group, the difference was statistically significant(P＜005). Good rate of granulation tissue growth, negative rate of bacterial culture,clearance rate of the wound bacterial of the observation group were obviously higher than those of the control group, while granulation tissue growth time, wound healing time and Visual Analogue Scale(VAS) score of the observation group were obviously lower than those of the control group, differences were statistically significant(P＜005). The incidence rates of pigmentation, scar hyperplasia, deepening and the infection of the wound of the observation group were obviously lower than those of the control group, differences were statistically significant(P＜005). Before treatment, there were no significant differences of the expression of IL1β and TNFα between the two groups(P＞005), and 14 days after treatment, the expression of IL1β and TNFα of the two groups were obviously lower, and those of the observation group were much lower than those of the control group, differences were statistically significant(P＜005).
〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓FGF+Collagen combined with mupirocin ointment in treating residual burn wounds has significant curative effect, which may effectively promote wound healing, prevent the scar hyperplasia. FGF+Collagen combined with Bactroban helps to inhibit bacterial growth by reducing the expression of wound inflammation factors, so as to reduce and avoid the incidence of wound infection. It is worthy of clinical application.


SHBG as a biomarker for prediction of gestational diabetes#br#

LI Zhijie， WU Yueqin

2017, 37(10):  1172-1174,1179.  doi:10.3969/j.issn.10073205.2017.10.013

Asbtract ( 471 )   PDF (382KB) ( 95 )  

Related Articles | Metrics

［Abstract］ Objective〖HTSS〗〓To explore the value of sex hormone binding globulin(SHBG) as a biomarker in prediction of gestational diabetes mellitus(GDM) at firsttrimester stage.
〖HTH〗〖WTHZ〗Methods〖HTSS〗〓Two hundred and sixty cases of pregnant women during the first trimester(13-16 weeks of gestation) were enrolled in this study. The content of SHBG in the serum was measured at 13-16 weeks of gestation and followed with oral glucose tolerance test at 24-28 weeks. The results of screening of GDM showed that 21 cases in GDM group, and 239 cases in normal control group. Age, body mass index(BMI), multipara, family history of diabetes, gestational weeks, delivery, cesarean section and neonatal amniotic fluid index, body mass and SHBG content were compared in 2 groups of pregnant women.
〖HTH〗〖WTHZ〗Results〖HTSS〗〓The ratio of BMI, cesarean section and neonatal body weight in GDM group were higher than that in normal control group, while the production of gestational week, amniotic fluid index and SHBG content were lower than that of normal control group(P<0.05). ROC curve analysis(AUC=0.877, 95% CI=0.806-0.948) found that when the cutoff value of SHBG was 175.86 nmol/L, the predictive sensitivity was 90.48%, the specificity was 66.94%, the positive predictive value was 19.19%, and the negative predictive value was 98.78%.
〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓Serum SHBG content in early pregnancy(13-16 weeks) can be used as a biomarker to predict GDM effectively.


MetaAnalysis of the effect of laser combined with intravitreal Ranibizumab versus laser alone in macular edema caused by branch retinal vein occlusion#br#

ZHANG Peng, MA Jingxue*， SHANG Qingli

2017, 37(10):  1175-1179.  doi:10.3969/j.issn.10073205.2017.10.014

Asbtract ( 473 )   PDF (1638KB) ( 88 )  

Related Articles | Metrics

〗［Abstract］  Objective〖HTSS〗〓To systematically evaluate the clinical effects of laser combined with intravitreal ranibizumab and laser alone in patients with branch retinal vein occlnsion(BRVO) complicated by macular edema(ME).
〖HTH〗〖WTHZ〗Methods〖HTSS〗〓Databases including Pubmed, The Cochrane Library(Issue 11, 2012), EMbase, CBM, CNKI, VIP and WanFang Data were electronically searched for the trials about the effects of laser combined with intravitreal ranibizumab and laser alone in patients with BRVO complicated by ME from the date of establishment of the databases to November 2015. Metaanalysis was performed with RevMan 5.3 software.
〖HTH〗〖WTHZ〗Results〖HTSS〗〓A total of 6 trials involving 208 petients and 218 eyes were included. Metaanalysis showed that there was significant statistical difference in best corrected visual acuity and central macular thickness between laser combined with intravitreal ranibizumab and laser alone in the 6month followup(MD=-0.130, 95%CI=-0.140 to -0.120, P<0.000 01)(MD=229.580, 95%CI=209.190-249.960, P<0.000 01).
〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓Current evidence showed the effects of laser combined with intravitreal ranibizumab were better than that of laser alone in improving best corrected visual acuity and decreasing central macular thickness among BRVO.


The expressions and significance of HSP90α and Ki67 in golden hamster buccal mucosa precancerous lesions after Huashi Xingyu Qingre decoction treatment#br#

ZHANG Pengfei1, LIU Jian2, LIU Tiejun2, XU Yanzhi2*

2017, 37(10):  1180-1183,1188.  doi:10.3969/j.issn.10073205.2017.10.015

Asbtract ( 388 )   PDF (2078KB) ( 81 )  

Related Articles | Metrics

［Abstract］ Objective〖HTSS〗〓To investigate the expressions and significance of heat shock protein 90α, HSP90α）and Ki67 in hamster buccal mucosa precancerous lesions after Huashi Xingyu Qingre decoction treatment.
〖HTH〗〖WTHZ〗Methods〖HTSS〗〓Sixty golden hamster models of buccal mucosa precancerous lesions were established after the contact of dimethylolbutanoie acid(DMBA) for six weeks. They were randomly divided into experimental and control groups. Thirty rats of experimental group were received gavage therapy of Huashi Xingyu Qingre decoction for 8 weeks, while, the other thirty rats of control group were received normal saline treatment for 8 weeks. The expression levels of HSP90α、Ki67 were assessed by dyeing intensity of streptavidinperosidase(SP).
〖HTH〗〖WTHZ〗Results〖HTSS〗〓The positive expression rate of Ki67 after gavage was 23.3%(7/30) in the experimental group and 80.0%(24/30) in the control group(P＜0.01). The positive expression rate of HSP90α after gavage was 20.0%(6/30) in the experimental group and 76.7%(23/30) in the control group(P＜0.01).
〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓Huashi Xingyu Qingre decoction is conspicuously effective in the reversal of DMBAinduced precancerous lesions in the hamster buccal mucosa.


Diagnostic study of different pathological types of nodular nodules with vascular and bronchial classification#br#

ZHANG Chenguang1, LIU Hao2, LI Shujing1*, SUN Mengyue1, ZHU Zhenlong3, LI Jianke4

2017, 37(10):  1184-1188.  doi:10.3969/j.issn.10073205.2017.10.016

Asbtract ( 417 )   PDF (381KB) ( 96 )  

Related Articles | Metrics

［Abstract］ Objective〖HTSS〗〓To classify the relationship between the subsolid nodules(SSNs) and the bronchi and the surrounding vessels in different pathological types, and to investigate the diagnostic value of benign and malignant nodules.
〖HTH〗〖WTHZ〗Methods〖HTSS〗〓Multislice spiral CT imaging of 101 subsolid nodules were retrospectively reviewed. According to the relationships between bronchus and the component of subsolid nodules, the types between subsolid nodules and bronchi were divided into five types. Depending on the vasculars' expansion or not and their tracks, the types between subsolid nodules and vasculars were categorized into four types. Statistical analysis was performed on the relationship between the types of vascular and brochi and subsolid nodules.
〖HTH〗〖WTHZ〗Results〖HTSS〗〓Of 47 benign subsolide nodules, brochus type Ⅴ and Ⅳ(27/47, 17/47) were the main relationships and the vascular type Ⅱ and Ⅲ(28/47,13/47) were the main relationships. Of 39 invasive adenocarcinomas, brochus type Ⅰ(21/39) was the main relationship and the vascular type Ⅲ and Ⅳ(23/39,12/39) were the main relationships. All of the nodules of premalignant group, microinvasive adenocarcinoma group and invasive adenocarcinoma group were not the brochus type Ⅴ and all of the nodules of microinvasive adenocarcinoma group and invasive adenocarcinoma group were not the vascular typeⅠ. There was significant statistic significance between the different pathology patterns of subsolid nodules and the lesion sizes, the types of brochi and vascular（P＜0.01）.
〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓Different types of bronchi and vascular predict different pathology patterns of subsolid nodules. It is important to predict the malignant and benign of subsolid nodules.


Clinical value of two scoring systems combined with high frequency color Doppler ultrasound in the diagnosis of acute appendicitis in children#br#

PANG Lin1, LIU Xuebin2

2017, 37(10):  1189-1192.  doi:10.3969/j.issn.10073205.2017.10.017

Asbtract ( 563 )   PDF (353KB) ( 58 )  

Related Articles | Metrics

［Abstract］ Objective〖HTSS〗〓To investigate the value of pediatric appendicitis score(PAS) and alvarado score system combined with high frequency color doppler ultrasonography for clinical diagnosis of children's acute appendicitis.
〖HTH〗〖WTHZ〗Methods〖HTSS〗〓Two hundred and fifty  cases of children with abdominal pain were accepted the examination of high frequency color ultrasonography and were tested with pediatric appendicitis score and alvarado score system after admission, and then were checked with pathological diagnosis after appendectomy. The sensitivity and specificity of 2 scoring systems combined with high frequency color doppler ultrasound for diagnosis of acute appendicitis in children were analyzed by ROC curve.
〖HTH〗〖WTHZ〗Results〖HTSS〗〓 Histopathological examination revealed 234 cases of acute purulent appendicitis(234/250, 93.6%) and 16 patients with acute simple appendicitis(16/250, 6.4%). The incidence of acute appendicitis by Alvarado and PAS scores was statistically significant among the lowrisk group, the middle risk group and the highrisk group(P>0.05). The critical diagnostic threshold for acute appendicitis was 6 points for the Alvarado and PAS scores. The sensitivity and specificity of Alvarado and PAS scoring in the diagnosis of acute appendicitis were 73.5% and 81.3%, 77.4% and 87.5% respectively. The sensitivity of combined with high frequency ultrasonography of acute appendicitis two scoring system(99.6%) was significantly higher than that of Alvarado score combined with high frequency ultrasound(82.1%)(χ2=6.327, P=0.020), PAS score combined with high frequency ultrasound(85.0%)(χ2=34.791, P=0.000). 
〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓The alvarado and PAS scoring system are valuable for the diagnosis of acute appendicitis in children, but the efficiency of 2 score combined with high frequency ultrasound diagnostic is better than a single scoring system or single scoring system combined with high frequency color doppler ultrasonography, which can improve the sensitivity and specificity of the diagnosis of children's acute appendicitis, and is worthy of clinical use.


Analysis of the value of AFP combined with abnormal prothrombin in the early diagnosis and curative effect evaluation of liver cancer#br#

LIN Bingzhu， LIU Xiaoyan

2017, 37(10):  1193-1196.  doi:10.3969/j.issn.10073205.2017.10.018

Asbtract ( 544 )   PDF (366KB) ( 141 )  

Related Articles | Metrics

［Abstract］  Objective〖HTSS〗〓To analyze the clinical value of combined detection of alphafetoprotein(AFP) and abnormal prothrombin(APT) in the early diagnosis and therapeutic evaluation of hepatocellular carcinoma.
〖HTH〗〖WTHZ〗Methods〖HTSS〗〓Fiftytwo patients with hepatocellular carcinoma(HCC) and 38 patients with cirrhosis were selected, and 30 healthy subjects were randomly selected as the control group.The detection values of AFP and APT were compared among 3 groups. The specificity, accuracy and sensitivity of AFP and APT single or combine detected for HCC were compared. The relationship between AFP and APT in serum of HCC was analyzed.
〖HTH〗〖WTHZ〗Results〖HTSS〗〓The values of AFP and APT in HCC patients were significantly higher than those in liver cirrhosis group and normal group(P＜005).The specificity of APT alone detection for HCC was significantly higher than that of AFP(P＜005). The sensitivity of the combined detection of AFP and APT for HCC was significantly higher than that in the single detection(P＜005).The specificity of combined detection of AFP and APT for HCC was significantly higher than that in AFP single detection(P＜005). The sensitivity of the combined detection of AFP and APT for HCC was significantly higher than that in AFP single detection(P＜005). The relationship of AFP and APT were independent, and there was no correlation between them through the analysis of scatter plots and Spearman correlation analysis(P>0.05).
〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓APT and AFP were important serological markers for clinical diagnosis of HCC, which could distinguish HCC and cirrhosis. Combined detection of AFP and APT could improve the diagnostic accuracy and sensitivity, and the diagnostic specificity remained at a high level. They have a profound diagnostic value for HCC.