Loading...

Table of Content

    25 September 2022, Volume 43 Issue 9
    Previous Issue    Next Issue
    Mechanism of lilarutide inhibiting inflammation of vascular smooth muscle cells via circ-sirt1/NF-κB signaling pathway
    LIU Yu, WANG Xing, CUI Hong-zhan, SUN Yong-quan, BU Ji-qiang, CHEN Zi-ying
    2022, 43(9):  996-1001.  doi:10.3969/j.issn.1007-3205.2022.09.002
    Asbtract ( 503 )   PDF (820KB) ( 366 )  
    Related Articles | Metrics
    ObjectiveTo investigate the effect of liraglutide(LIR) on tumor necrosis factor(TNF)-α-induced vascular smooth muscle cell inflammation and its possible molecular mechanism. 
    MethodsThe experiment was divided into three groups: blank control group(NC group), TNF-α group(10 μg/L) and TNF-α +LIR group(10 μg/L TNF-α + 100 nmol/L LIR). IL-1β and IL-6 in cell culture medium were detected by enzyme linked-immunosorbent assay(ELISA). The mRNA expressions of ICAM-1, MCP-1 and IκB-α and circ-sirt1 were detected by real-time quantitative polymerase chain reaction detecting system（qPCR）. The protein expressions of ICAM-1, MCP-1 and IκB-α were detected by Western blot. The protein distribution of NF-κB p65 was detected by immunofluorescence. 
    ResultsCompared with NC group, the levels of IL-1β and IL-6 in TNF-α group were significantly increased, and the mRNA and protein expressions of ICAM-1 and MCP-1 were significantly increased. The mRNA and protein expression of IκB-α was significantly decreased, and the expression of circ-sirt1 was significantly decreased. NF-κB p65 protein was mainly localized in the nucleus. Compared with TNF-α group, the levels of IL-1β and IL-6 and the protein expressions of ICAM-1 and MCP-1 were significantly decreased, while the protein expression of IκB-α was significantly increased in TNF-α+LIR group. Meanwhile，the expression of circ-sirt1 was significantly increased and nuclear localization of NF-κB p65 protein was decreased in TNF-α+LIR group. 
    ConclusionLilarutide can inhibit the inflammatory response of vascular smooth muscle cells, possibly by inhibiting the release of inflammatory mediators and the activation of circ-sirt1/NF-κB signaling pathway.

    Expressions of P38MAPK and caspase-3 in intracranial aneurysm and their relationship with prognosis
    GAO Jie, SHEN Xiao-juan
    2022, 43(9):  1002-1007.  doi:10.3969/j.issn.1007-3205.2022.09.003
    Asbtract ( 285 )   PDF (1250KB) ( 113 )  
    Related Articles | Metrics
    ObjectiveTo observe the expressions of mitogen-activated protein kinase p38(P38MAPK) and caspase-3 in the aneurysm wall of patients with intracranial aneurysm(ICA), and to analyze their relationship with ICA. 
    MethodsThirty patients(30 artery aneurysm wall tissues) with intracranial aneurysm who underwent craniotomy in our hospital were selected as the observation group. In addition, 20 patients(20 normal arterial vascular wall tissues) with cranial trauma(confirmed by CT and MRI as non-vascular diseases) who underwent craniotomy in our hospital during the same period were selected as the control group. The patients in the observation group were followed up for 12 months, and GOS score was used to evaluate the prognosis: 1-3 points were poor prognosis and 4-5 points were good prognosis. The expression of P38MAPK and caspase-3 in artery aneurysm wall tissues and normal arterial vascular wall was measured by immunohistochemistry, and the relationships between their expression and the clinicopathological characteristics of patients with ICA were analyzed. Spearman was used to analyze the correlation between the expression of P38MAPK and caspase-3 in ICA wall tissue in the observation group, and COX regression was used to analyze the factors affecting the prognosis of patients with intracranial aneurysm. 
    ResultsThe positive expression rate of P38MAPK in the observation group(60.00%) was significantly higher than that(10.00%) in the control group, and the positive expression rate of caspase-3(33.33%) was significantly lower than that in the control group(80.00%)(P＜0.05). The expressions of P38MAPK and caspase-3 in ICA wall tissue were related to the Hunt-hess grade(P＜0.05). Spearman analysis showed that there was a significant negative correlation between the expression of P38MAPK and caspase-3 in ICA wall tissue of the observation group(r=-0.513, P＜0.05). Multivariate COX regression analysis showed that Hunt-hess grade, high P38MAPK expression and low caspase-3 expression were independent risk factors for the prognosis of patients with ICA(P＜0.05). 
    ConclusionThe expression of P38MAPK is significantly higher and the expression of caspase-3 is significantly lower in the ICA wall. Both of them are significantly and negatively correlated with each other, and related to the Hunt-hess grade in patients. Combined detection of the two indicators may have certain reference value for the condition monitoring and prognosis evaluation of ICA.

    Effect of estimated glomerular filtration rate on 1-year mortality in patients with acute ischemic stroke aged ≥75 years
    ZHANG Yu-yu, LIU Zhong-zhong, ZHANG Na, LIN Xue-mei, WANG Fang, WU Song-di
    2022, 43(9):  1008-1013.  doi:10.3969/j.issn.1007-3205.2022.09.004
    Asbtract ( 302 )   PDF (576KB) ( 72 )  
    Related Articles | Metrics
    ObjectiveTo investigate the effect of estimated glomerular filtration rate(eGFR) level on 1-year mortality in patients aged ≥75 years with acute ischemic stroke(AIS). 
    MethodsClinical data of patients with acute stroke were included. Patients aged ≥75 years with AIS were selected and followed up for 3 months and 1 year respectively, and death events were recorded. The eGFR was treated as continuous variable and quartile grouped variable(group Q1-Q4). Multivariate Cox regression analysis was performed on the effect of eGFR level on 1-year mortality in patients aged ≥75 years with AIS. 
    ResultsA total 506 patients aged ≥75 years with AIS were enrolled. The 3-month and 1-year mortality of Q1 group was significantly higher than that of other groups during follow-up, with statistical significance(P＜0.001). After adjustment for the related confounders, multivariate Cox regression analysis showed that for every 5 mL·min-1·（1.73 m2）-1 increase in eGFR level, the risk of 1-year mortality in patients aged ≥75 years with AIS decreased by 12.3%(HR=0.877, 95%CI: 0.8123-0.935，P＜0.001). Compared with the Q4 group, the risk of 3-month and 1-year mortality in group Q1 was significantly increased by 1.890 and 1.267 times, respectively(3 months: HR=2.890, 95%CI:1.010-8.241，P=0.048; 1-year: HR=2.267, 95%CI:1.118-4.596，P=0.023). Kaplan-Meier survival analysis found a significant decrease in survival in the Q1 group compared to the other groups. 
    ConclusionReduced eGFR level is a risk factor for 1-year mortality in patients aged ≥75 years with AIS, and eGFR≤60.2 mL·min-1·（1.73 m2）-1 significantly increases the risk of 1-year mortality in patients aged ≥75 years with AIS. 

    Role of LAG-3 and tumor infiltrating lymphocytes in the prognosis of patients with esophageal squamous cell carcinoma
    LIU Yao, JIN Jing, SUN Xue-mei, LIU Jing, WANG Xiao-xiao, LIU Yue-ping
    2022, 43(9):  1014-1019,1024.  doi:10.3969/j.issn.1007-3205.2022.09.005
    Asbtract ( 384 )   PDF (2907KB) ( 140 )  
    Related Articles | Metrics
    ObjectiveTo investigate the expression and prognostic value of lymphocyte activating gene 3（LAG-3） and tumor infiltrating lymphocytes(TILs) in esophageal squamous cell carcinoma(ESCC). 
    MethodsThe hematoxylin-eosin staining(HE) sections of 60 patients with ESCC who were not treated before operation in our hospital were analyzed retrospectively, and the TILs HE staining was interpreted. Meanwhile, immunohistochemical staining for LAG-3 was performed, and the results were observed and interpreted under the light microscope. The expression of LAG-3 and TILs in ESCC was analyzed. The correlation and their function in the prognosis of patients with ESCC were statistically analyzed. 
    ResultsImmunohistochemical results showed that the positive rate of LAG-3 in ESCC was 38.3%(23/60), and LAG-3 was only expressed on TILs, but not on tumor cells. Reverse transcription polymerase chain reaction(RT-PCR) showed that the relative expression of LAG-3 in ESCC was higher than that in adjacent normal esophageal mucosa. In 60 patients with ESCC, there were different degrees of tumor lymphocyte infiltration, including 21 cases of low TILs, 27 cases of moderate TILs and 12 cases of high TILs. The expression of LAG-3 was significantly correlated with TILs, tumor differentiation and TNM stage. Kaplan Meier survival analysis showed that the PFS was related to TNM stage, lymph node status, LAG-3 and TILs, suggesting significant differences(P＜0.05). Multivariate COX regression analysis showed that the PFS was related to TNM stage and LAG-3, and the difference was statistically significant(P＜0.05). 
    ConclusionThe high expression of LAG-3 is associated with the improvement of survival rate, and LAG-3 is an independent predictor of survival, suggesting that LAG-3 may be a useful immune marker for the prognosis of ESCC.

    Predictive value and correlation analysis of CCK, Cx43 and GLP-1 in gastric emptying in patients with type 2 diabetic gastroparesis
    ZHANG Xue-lian, WANG Bo, ZHANG Xuan-e, ZHANG Xiao-yan, GU Lei, ZHOU Zun-hai
    2022, 43(9):  1020-1024.  doi:10.3969/j.issn.1007-3205.2022.09.006
    Asbtract ( 376 )   PDF (636KB) ( 132 )  
    Related Articles | Metrics
    ObjectiveTo investigate the predictive value and correlation of cholecystokinin(CCK), connexin 43(Cx43) and glucagon-like peptide-1(GLP-1) in gastric emptying in patients with type 2 diabetic gastroparesis(DGP). 
    MethodsIn total, 127 patients with type 2 diabetes with abnormal gastric function were selected, including 49 patients with functional dyspepsia as dyspepsia group, and 78 patients with diabetic gastroparesis as gastroparesis group. Another 50 healthy patients with normal gastric function who underwent physical examination during the same period were selected as control group. The gastric half-emptying time was measured in all subjects. CCK, Cx43 and GLP-1 levels were detected by enzyme-linked immunosorbent assay, and solid gastric emptying ability was detected by radionuclide. The predictive value of CCK, Cx43 and GLP-1 in gastric emptying in patients with type 2 DGP, and the correlation of CCK, Cx43 and GLP-1 were analyzed. 
    ResultsThe levels of CCK and Cx43 in dyspepsia group and gastroparesis group were lower than those in control group, the levels of GLP-1 were higher than those in control group, and the gastric half-emptying time was longer than that in control group; the levels of CCK and Cx43 in gastroparesis group were lower than those in dyspepsia group, the levels of GLP-1 were higher than those in dyspepsia group, and the gastric half-emptying time was longer than that in dyspepsia group(P＜0.05). The combined detection of CCK, Cx43 and GLP-1 had higher predictive value for gastric emptying in patients with type 2 DGP. CCK was positively correlated with Cx43(r=0.482; P＜0.001), CCK was negatively correlated with GLP-1(r=-0.356; P＜0.001), and Cx43 was negatively correlated with GLP-1(r=-0.619; P＜0.001). 
    ConclusionCCK, Cx43 and GLP-1 are abnormally expressed in type 2 DGP, which has certain value in predicting type 2 DGP.

    Study on skeletal muscle content and its influencing factors in 91 patients with kidney disease
    CAO Ying, YUAN Guo-hai, ZHANG Ye, WANG Chao-jun, LIU Ju, JIN You-you
    2022, 43(9):  1025-1028.  doi:10.3969/j.issn.1007-3205.2022.09.007
    Asbtract ( 367 )   PDF (377KB) ( 71 )  
    Related Articles | Metrics
    ObjectiveTo explore the skeletal muscle content and its influencing factors in patients with kidney disease. 
    MethodsA cross-sectional study was performed to collect complete information of 91 patients with kidney disease for body composition testing. Unconditional Logistic regression analysis was performed to analyze the influencing factors of skeletal muscle in patients with kidney disease. 
    ResultsSkeletal muscle content of 91 patients was （25.68 ± 6.07） kg, including 32 persons(35.2%) in the low skeletal muscle group. There were significant differences in age, creatine(Cr), blood urea nitrogen(BUN), albumin(ALB), urine protein and height between the non-low skeletal muscle group and the low skeletal muscle group(P＜0.05). There was no significant difference in gender, smoking, drinking, hypertension, diabetes, estimated glomerular filtration rate(eGFR), Cr, prealbumin(PA), triglyceride(TG) and hemoglobin(Hb)(P＞0.05). Multivariate Logistic regression analysis showed that Cr and ALB were independent risk factors for the low skeletal muscle content, while proteinuria and height were protective factors in patients with kidney disease. 
    ConclusionSkeletal sarcopenia is common in kidney disease and a good indicator to assess the nutritional status of patients. The important effect of reasonable nutritional therapy on skeletal muscle should warrant attention in all stages of kidney disease.

    Influencing factors of nosocomial infection in schizophrenic inpatients
    YUAN Jing, SUN Lian-fang
    2022, 43(9):  1029-1032.  doi:10.3969/j.issn.1007-3205.2022.09.008
    Asbtract ( 319 )   PDF (362KB) ( 185 )  
    Related Articles | Metrics
    ObjectiveTo analyze the influencing factors of nosocomial infection in schizophrenic patients. 
    MethodsA total of 3 579 inpatients with schizophrenia admitted to the Psychiatric Department of our hospital were selected retrospectively, and the incidence of nosocomial infections was recorded. The clinical baseline data, including age, sex, length of hospital stay, clinical treatment, concomitant diseases and cognitive ability, were collected, and the risk factors of nosocomial infection in schizophrenic patients were analyzed by Logistic regression analysis. 
    ResultsThere were 67 cases of nosocomial infection in 3 579 patients, and the infection rate was 1.87%. There were significant differences in age, duration of schizophrenia, length of hospital stay, electroconvulsive therapy, management style, cognitive impairment, self-care and concomitant diseases between two groups(P＜0.05), while there were no statistically significant differences in gender and education level(P＞0.05). Logistic regression analysis showed that age was the protective factor for nosocomial infection in schizophrenic patients, while cognitive impairment, combined diabetes mellitus, and course of disease were risk factors for nosocomial infection in schizophrenic patients. 
    ConclusionIn order to prevent nosocomial infection, the intervention should be strengthened for the elderly patients with long course of disease, cognitive impairment and schizophrenia complicated with diabetes. 

    Effect of two-stage citrate anticoagulation combined with CVVH on inflammatory factors and serum HO-1 in patients with acute liver and kidney injury after cardiovascular surgery
    TANG Zheng-wen, ZHOU Ying, GU Yang, ZHANG Li-qin
    2022, 43(9):  1033-1036,1051.  doi:10.3969/j.issn.1007-3205.2022.09.009
    Asbtract ( 456 )   PDF (407KB) ( 140 )  
    Related Articles | Metrics
    ObjectiveTo study the effect of two-stage citrate anticoagulation combined with continuous veno venous hemofiltration(CVVH) on inflammatory factors and serum HO-1 in patients with acute liver and kidney injury after cardiovascular surgery. 
    MethodsA total of 112 patients with acute liver and kidney injury after cardiovascular surgery requiring CVVH were selected and divided into the control group(n=56) and the research group(n=56) according to the random table method. The control group was given heparin for anticoagulation, and the research group was given two-stage citrate anticoagulation(sodium citrate was given for anticoagulation in front of the filter and at the venous pot). Anticoagulant effectiveness,the service time of the filter, serum inflammatory factors and HO-1 between two groups were compared. In addition, adverse reactions were compared in the two groups. 
    ResultsThere was no significant difference in anticoagulant effectiveness between two groups after treatment(P＞0.05). The service life of the filter in the research group was longer than that in the control group(P＜0.05). The prothrombin time(PT), activated partial thromboplastin time(APTT), and thrombin time(TT) of the two groups after treatment were longer than those before treatment, and longer in the research group than in the control group(P＜0.05). After treatment, C-reactive protein(CRP), interleukin-6(IL-6), tumor necrosis factor-α(TNF-α), and malondialdehyde(MDA) of the two groups were lower than those before treatment, which were lower in the research group than in the control group(P＜0.05). After treatment, the HO-1 levels of the two groups decreased compared with those before treatment, and the research group showed lower results(P＜0.05). The research group had lower incidence of complications than control group(P＜0.05). 
    ConclusionTwo-stage citrate anticoagulation combined with CVVH in the treatment of patients with acute liver and kidney injury after cardiovascular surgery can reduce the level of inflammatory factors in the body, increase the service time of the filter, reduce the level of HO-1 in the body, and lower the risk of bleeding.

    Comparison of the application effect of open and closed reduction and internal fixation in posterior malleolus fractures via posterolateral approach
    LYU Yan-min
    2022, 43(9):  1037-1041.  doi:10.3969/j.issn.1007-3205.2022.09.010
    Asbtract ( 319 )   PDF (391KB) ( 173 )  
    Related Articles | Metrics
    ObjectiveTo observe and compare the application effect and safety of open and closed reduction and internal fixation in posterior malleolus fractures via posterolateral approach. 
    MethodsThe clinical data of 87 patients with ankle fracture combined with posterior malleolus fracture were retrospectively analyzed, and they were divided into the open group(n=44) and the closed group(n=43) according to different treatment methods. The open group was treated with open reduction and internal fixation via the posterolateral approach, and the closed group was treated with closed reduction and internal fixation. Operation-related indexes, complications, excellent and good rate of ankle joint function recovery at 12 months after operation, bone metabolism indexes before operation, at 2 weeks and 4 weeks after operation［type Ⅰ collagen carboxy-terminal peptide β special sequence(β-CTX), procollagen type Ⅰ carboxy-terminal peptide(PICP), bone gla protein(BGP)］,and ankle range of motion(ROM)at 6 months and 12 months after surgery were compared. 
    ResultsThe duration of operation and fracture healing time of the closed group were shorter than those of the open group, and the intraoperative blood loss was less than that of the open group(P＜0.05).Serum β-CTX levels in the two groups showed a gradually decreasing trend, while PICP and BGP levels gradually increased.The difference of interaction between groups, time points and time points between groups were statistically significant(P＜0.05). The incidence of postoperative complications in the closed group was lower than that in the open group(P＜0.05). There was no significant difference in the ROM of plantar flexion, dorsiflexion, valgus and varus between the two groups at 6 and 12 months after operation(P＞0.05).There was no significant difference in the excellent and good rate of ankle function recovery between two groups at 12 months after operation(P＞0.05). 
    ConclusionCompared with open reduction and internal fixation via the posterolateral approach, closed reduction and internal fixation for the treatment of posterior ankle fractures has the advantages of convenient operation, less trauma, fewer complications, improved postoperative bone metabolism indexes, and faster fracture healing. Ankle joint function has recovered well.

    The relationship between serum GLP-1 and IGF-1R in placental tissues and neonatal outcome in patients with gestational diabetes
    SHAO Jie, YANG Mei-fang, ZHANG Xiu
    2022, 43(9):  1042-1046.  doi:10.3969/j.issn.1007-3205.2022.09.011
    Asbtract ( 405 )   PDF (398KB) ( 73 )  
    Related Articles | Metrics
    ObjectiveTo study the relationship between serum glucagon-like peptide-1(GLP-1) and insulin-like growth factor 1 receptor(IGF-1R) in placental tissues and neonatal outcomes in patients with gestational diabetes. 
    MethodsPregnant women undergoing pregnancy examination and women with full-term singleton pregnancy in our hospital were enrolled. They underwent 75 g OGTT examination at 24-28 weeks of pregnancy and were divided into GDM group and non-GDM group. Body mass index(BMI), age, gestational age, fasting plasma glucose(FPG), 1 h fasting plasma glucose after taking sugar(FPG1), 2 h fasting plasma glucose after taking sugar(FPG2), IGF-1R, glycosylated hemoglobin(HbA1c), and GLP-1 were compared between the two groups. Meanwhile, the relationship between GLP-1, IGF-1R and neonatal outcomes as well as the correlation between GLP-1 and IGF-1R was explored. 
    ResultsThere were significant differences in BMI, FPG, FPG1, FPG2, IGF-1R, HbA1c, and serum GLP-1 between two groups before pregnancy(P＜0.05), but there was no difference in age and gestational age(P＞0.05). In the GDM group, 50 cases(25.0%) had adverse neonatal outcomes, and 10 cases(5%) in the non-GDM group had adverse neonatal outcomes, suggesting significant differences(χ2=31.373, P＜0.001). GLP-1 had a negative impact on adverse neonatal outcomes ［β=-0.141, OR=0.869(95%CI: 0.821-0.919)］, and IGF had a positive impact on adverse neonatal outcomes［β=0.745, OR= 2.107(95%CI: 1.214-3.656)］. The independent risk factors affecting adverse neonatal outcomes were GDM and IGF-1R. There was a negative correlation between GLP-1 and IGF-1R(r=-0.478, P＜0.05). 
    ConclusionSerum GLP-1 in patients with gestational diabetes negatively affects adverse neonatal outcomes, while IGF-1R in placental tissues positively affects adverse neonatal outcomes, which warrants attention in clinical practice.

    The value of neonatal lung ultrasound in the evaluation of pulmonary infection in premature infants
    DONG Ya-qing, GAO Zhi-min, XU Ran, CHEN Li-fang, YUAN Jing, WANG Ming-rong
    2022, 43(9):  1047-1051.  doi:10.3969/j.issn.1007-3205.2022.09.012
    Asbtract ( 330 )   PDF (584KB) ( 143 )  
    Related Articles | Metrics
    ObjectiveTo explore the value of neonatal pulmonary ultrasound in the evaluation of pulmonary infection in premature infants. 
    MethodsIn total, 80 preterm infants with pulmonary infection were selected as the observation group, and 80 preterm infants without pulmonary infection were selected as the control group. All of them were given neonatal pulmonary ultrasound examination. The pulmonary ultrasound score, oxygenation index and respiratory index of the two groups were compared. The pulmonary ultrasound score, oxygenation index and respiratory index of the children with different conditions and prognosis in the observation group were compared, and the correlation between the pulmonary ultrasound score, oxygenation index and respiratory index was analyzed. 
    ResultsThe pulmonary ultrasound score and oxygenation index of the observation group were lower than those of the control group, and the respiratory index was higher than that of the control group(P＜0.05). In the observation group, the pulmonary ultrasound score and oxygenation index of extremely critical children were lower than those of critical and non-critical children, and the respiratory index was higher than that of critical and non-critical children. The pulmonary ultrasound score and oxygenation index of critical children were lower than those of non-critical children, while the respiratory index was higher than that of non-critical children, suggesting significant differences(P＜0.05). The pulmonary ultrasound score was negatively correlated with respiratory index(r=-0.455, P＜0.05) and positively correlated with oxygenation index(r=0.470, P＜0.05). In the observation group, the pulmonary ultrasound score and oxygenation index of the dead children were lower than those of the surviving children, and the respiratory index was higher than that of the surviving children, with a significant difference(P＜0.05). The area under receiver operating characteristic(ROC) curve of pulmonary ultrasound score for predicting prognosis of children was 0.874, which was higher than that of respiratory index and oxygenation index(P＜0.05). When the cutoff value was 20 points, the sensitivity and specificity were 88.50% and 75.00% respectively. 
    ConclusionNeonatal pulmonary ultrasound has a good effect in the evaluation of premature infants with pulmonary infection, which is worthy of clinical application.

    Expression of CYP1A1, TSLPR and CCR3 in atopic dermatitis in children and its correlation with clinical characteristics
    LING Wan-ming, LIU Yan, GU Hong-xia, ZHAO Rui-xue, HAN Zhi-min
    2022, 43(9):  1052-1056,1061.  doi:10.3969/j.issn.1007-3205.2022.09.013
    Asbtract ( 333 )   PDF (565KB) ( 105 )  
    Related Articles | Metrics
    ObjectiveTo investigate the expression of cytochrome P4501A(CYP1A1), thymic stromal lymphopoietin receptor(TSLPR) and CC chemokine receptor 3(CCR3) in children with atopic dermatitis and to analyze their correlation with clinical characteristics. 
    MethodsA total of 89 children with atopic dermatitis treated in our hospital were selected as atopic dermatitis group, and 80 healthy children who underwent physical examination in our hospital during the same period were selected as healthy group. Three mL of fasting venous blood was collected from the two groups on the 2nd day after enrollment, and peripheral blood mononuclear cells were obtained under density gradient centrifugation. The expressions of CYP1A1 and TSLPR were detected by real-time quantitative PCR(RT-qPCR), and CCR3 expression in peripheral blood mononuclear cells was measured by fluorescent antibody labeling and flow cytometry. The expressions of CYP1A1, TSLPR and CCR3 in children with atopic dermatitis and their correlation with clinical characteristics were analyzed. 
    ResultsCompared with healthy group, the expressions of CYP1A1, TSLPR and CCR3 in atopic dermatitis group increased(P＜0.05). The expressions of CYP1A1, TSLPR and CCR3 were not correlated with age, gender and accompanying symptoms of atopic dermatitis, suggesting no significant difference(P＞0.05). The expressions of CYP1A1, TSLPR and CCR3 were correlated with disease severity, family allergy history, family history of atopic dermatitis, and personal allergic history. Compared with the patients with mild and moderate atopic dermatitis, no family history of atopic dermatitis, no family history of atopic dermatitis and no personal allergy, the expression of CYP1A1, TSLPR and CCR3 in patients with severe atopic dermatitis, family history of allergy, family history of atopic dermatitis, and personal history of allergy were significantly higher(P＜0.05). Pearson correlation analysis showed positive correlation between CYP1A1, TSLPR and CCR3(P＜0.05). ROC curve analysis of the predictive value of CYP1A1, TSLPR and CCR3 in children with atopic dermatitis showed that combined detection of CYP1A1, TSLPR and CCR3 in predicting atopic dermatitis in children was higher than that of CYP1A1, TSLPR and CCR3 alone(P＜0.05). 
    ConclusionThe expressions of CYP1A1, TSLPR and CCR3 in children with atopic dermatitis increase, which is related to the severity of the disease, family history of allergy, family history of atopic dermatitis, and personal history of allergy. Therefore, combined detection of the three indicators can be used to predict atopic dermatitis in clinical practice.

    Clinical application of minimally invasive cochlear implantation for residual hearing protection
    HE Qiang, JIA Qiao-jing, HAN Hai-xia, ZHANG Ying, JIA Zhan-wei
    2022, 43(9):  1057-1061.  doi:10.3969/j.issn.1007-3205.2022.09.014
    Asbtract ( 360 )   PDF (403KB) ( 58 )  
    Related Articles | Metrics
    ObjectiveTo clarify the hearing recovery and residual hearing protection of patients with cochlear malformation and those with normal cochlea but requiring cochlear implantation after minimally invasive cochlear implantation.  
    MethodsThe patients were grouped and included by pre-operative temporal bone CT, inner ear hydrography and audiology examination. The data of 38 patients undergoing minimally invasive cochlear implantation were analyzed, including 16 cases of cochlear malformation and 22 cases of normal cochlea. We compared the categories of auditory performance(CAP) and speech intelligibility rating(SIR) scores and residual hearing retention between two groups after cochlear implantation. The criteria for hearing retention were as follows: the mean thresholds of 125, 250 and 500 Hz were ＜10 dBHL higher than those before implantation. 
    ResultsThe electrode of artificial cochlea was successfully implanted, and the electrode impedance was detected normally during the operation. After startup, the cochlear implant worked normally and well in both groups. In 16 cochlear implantation patients with cochlear malformation, the low-frequency residual hearing retention rate was 82%, complete hearing retention rate was 33%, and partial hearing retention rate was 49% at 1 year after cochlear implantation. In 22 patients with normal cochlea, low-frequency residual hearing retention rate was 100%, complete hearing retention rate was 82%, and partial hearing retention rate was 18%. There was no change in hearing retention rate between 6-months and 1-year follow-up compared with the data at 1 month. The postoperative CAP and SIR scores were 5.7±1.2 and 3.5±1.0 in patients with cochlear malformation respectively, and 6.4±1.1 and 3.7±0.9 in patients with normal cochlea respectively, and the difference was not statistically significant(P＞0.05). 
    ConclusionThere is no significant difference in speech recovery and response ability to voice between patients with cochlear malformation and those with normal cochlea. However, the retention rate of low-frequency residual hearing in cochlear malformation group decreased to a certain extent due to the influence of the cochlear structure. In this study, there is no significant difference in postoperative efficacy and retention of residual hearing between patients with cochlear malformation and those with normal cochlea after minimally invasive cochlear implantation, which provides guidance for clinical diagnosis and treatment of such patients.

    The expression of OPG, IL-35 and IL-37 in gingival crevicular fluid in chronic periodontitis and their correlation with alveolar bone resorption
    LIU Xiao-yan, GENG Wen-hua, DU Sha-sha, LIU Ying-qi
    2022, 43(9):  1068-1072,1078.  doi:10.3969/j.issn.1007-3205.2022.09.016
    Asbtract ( 371 )   PDF (480KB) ( 111 )  
    Related Articles | Metrics
    ObjectiveTo explore the expression of osteoprotegerin(OPG), interleukin-35(IL-35) and interleukin-37(IL-37) in chronic periodontitis and their correlation with alveolar bone resorption. 
    MethodsIn total, 105 patients with chronic periodontitis were selected as the experimental group, 45 patients with chronic periodontitis were selected as the verification group, and 105 patients undergoing physical examination were selected as the control group. The levels of OPG, IL-35 and IL-37 in the gingival crevicular fluid of the experimental group and the control group were compared. The proportion of alveolar bone resorption in patients with different clinical characteristics in the experimental group was compared. The influencing factors of resorption ratio of the alveolar bone in the experimental group were analyzed. A multiple linear regression model was established. 
    ResultsThe levels of OPG and IL-37 in gingival crevicular fluid of the experimental group were lower than those of the control group, while the level of IL-35 was higher than that of the control group(P＜0.05). The results of multivariate linear regression analysis showed that the levels of OPG, IL-35 and IL-37 in gingival crevicular fluid were the influencing factors of resorption ratio of the alveolar bone(P＜0.05). The regression model was established as follows: Resorption ratio of the alveolar bone=6.495-0.180×OPG+0.169×IL-35-0.055×IL-37. The independent variable could explain the 91.5% variation of resorption ratio of the alveolar bone. The regression model was validated with the OPG, IL-35, and IL-37 levels in the patients′ gingival crevicular fluid in the verification group, and the mean absolute deviation(MAD) value was 1.25%. 
    ConclusionThe expression of OPG and IL-37 in the gingival crevicular fluid of patients with chronic periodontitis is low, and the expression of IL-35 is high. The three are closely related to alveolar bone resorption.

    Clinical comparison of Erbium Yttrium-Aluminum-Garnet laser and piezosurgery in the extraction of low vertically impacted mandibular third molars
    GUO Tao, ZHANG Peng-fei, XU Yin, YANG Ting, ZHANG Yu-yin
    2022, 43(9):  1073-1078.  doi:10.3969/j.issn.1007-3205.2022.09.017
    Asbtract ( 373 )   PDF (849KB) ( 174 )  
    Related Articles | Metrics
    ObjectiveTo compare the clinical effect of Erbium Yttrium-Aluminum-Garnet(Er：YAG) laser and piezosurgery(PS) in removing the bone resistance of low vertically impacted mandibular third molars, and to explore the optimal method for extraction of such teeth. 
    MethodsA total of 60 eligible patients were randomly divided into two groups for osteotomy: Er：YAG laser group and PS group. The duration of operation, postoperative pain, facial edema, trismus and patient satisfaction were compared between the two groups. 
    ResultsThe postoperative pain at 1, 3, and 7 d after surgery，and facial edema and trismus at 3 and 7 d after surgery were slightly higher in Er：YAG laser group than in PS group, suggesting no significant difference. However, the duration of operation was significantly shorter and the patient satisfaction was higher in PS group. 
    ConclusionIn the extraction of low vertically impacted mandibular third molars, the postoperative complications between Er：YAG laser and PS groups are similar, but PS is more efficient and obtains more patient satisfaction.

    Safety research of low-dose hypobaric bupivacaine for spinal anesthesia for hip surgery in elderly patients
    WANG Chun-xiao, LI Zhi-hua, XU Guan-jie, ZHANG Xiu-ning, QIE Xiao-juan, HAO Li-jing
    2022, 43(9):  1079-1084.  doi:10.3969/j.issn.1007-3205.2022.09.018
    Asbtract ( 322 )   PDF (429KB) ( 91 )  
    Related Articles | Metrics
    ObjectiveTo compare the safety and effectiveness of different doses of hypobaric bupivacaine for spinal anesthesia for hip surgery in elderly patients, in order to screen the best clinical safety dose. 
    MethodsSixty elderly patients with hip fracture of American Society of Anesthesiologists(ASA) Ⅱ-Ⅲ were scheduled for elective hip surgery. According to the random number table method, the patients were divided into low dose group(group L),medium dose group(group M) and high dose group(group H), with 20 cases in each group. The affected side was kept in the upper lateral supine position, and subarachnoid block anesthesia was performed in the L3-4 in tervertebral space. After successful puncture, 0.375% bupivacaine(0.75% bupivacaine + sterilized water for injection) was injected into the subarachnoid space, and the doses were calculated according to unit height of 0.033 mg/cm,0.05 mg/cm, and 0.08 mg/cm, respectively. Changes of mean arterial pressure(MAP) and heart rate(HR) at entry(T0) and at each time point(T1-T5) after administration in the three groups were recorded. Intraoperative use of vasoactive drugs, the onset time of anesthesia, fixation time of the anesthesia plane, the number of anesthesia block segments on the affected side, and the duration of anesthesia block, and the occurrence of complications during and after operation were recorded and compared among the three groups. 
    ResultsThe frequency of vasoactive drugs in group H was higher than that in groups L and M, and the difference was statistically significant(P＜0.05). After injection, MAP in the three groups showed a trend of initial decrease and subsequent increase, with the greatest fluctuation in MAP and HR in group H, and there were statistically significant differences in the interaction among groups, time points, and time points among groups(P＜0.05). The onset time of anesthesia and the fixation time of anesthesia plane in group H were shorter than those in groups L and M,and shorter in group M than in group L(P＜0.05); The duration of anesthesia and analgesia was longer than groups L and M(P＜0.05). The Bromage score of group H was higher than that of groups L and M at 1 d after operation(P＜0.05). The MMSE scores of the three groups showed a trend of initial decrease and subsequent increase, with the greatest fluctuation in group H, and there were statistically significant differences in the interaction among groups, time points, and time points among groups(P＜0.05). The incidence rate of intraoperative hypotension and sinus bradycardia in group H was higher than that in groups L and M, and the differences were statistically significant(P＜0.05). 
    ConclusionLow-dose(0.033 mg/cm) light specific gravity lumbar anesthesia with bupivacaine can be safely and effectively used in hip surgery in elderly patients which is the recommended dose for clinical safety.

    2022, 43(9):  1108-1103.  doi:10.3969/j.issn.1007-3205.2022.09.025
    Asbtract ( 479 )   PDF (427KB) ( 72 )  
    Related Articles | Metrics